Paleoparasitologic, paleogenetic and paleobotanic analysis of XVIII century coprolites from the church La Concepción in Santa Cruz de Tenerife, Canary Islands, Spain

Botella, Herminia Gijón; Vargas, José A Afonso; Rosa, Manuel Antonio González de la; Leles, Daniela; Reimers, Emilio González; Vicente, Ana Carolina Paulo; Iñiguez, Alena Mayo

doi:10.1590/s0074-02762010000800017

Cited by 23 publications

(12 citation statements)

References 13 publications

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“…eggs using 18S rRNA (Loreille et al, 2001;Botella et al, 2010;Oh, Seo, Lim et al, 2010). Here, additional Ascaris 18S rRNA reference sequences were generated, as very few are available in the nucleotide database at NCBI.…”

Section: Morphological Examinationmentioning

confidence: 99%

“…has limited aDNAbased studies to identifying genera. Previous studies have targeted Ascaris 18S rRNA in ancient eggs samples presumably of human origin and found the highest sequence homology to sequences from the pig roundworm A. suum U94367 (Loreille et al, 2001;Oh, Seo, Lim et al, 2010) and AF036587 (Botella et al, 2010). In the case of the studies based on the U94367 sequence, the A. lumbricoides sequence U94366 was used as the only speciesspecific sequence, while Botella et al (2010) only reported on homology to the A. suum sequence AF036587.…”

Section: Paleoparasitologymentioning

confidence: 99%

“…Loreille et al (2001) were the first to report on ancient DNA (aDNA) isolated from parasite egg remains. Since then, aDNA analysis has increasingly been applied to paleoparasitological studies (I˜niguez et al, 2003;Leles et al, 2008Leles et al, , 2014Botella et al, 2010;Oh, Seo, Chai et al, 2010;Oh, Seo, Lim et al, 2010;Cleeland et al, 2013;Shin et al, 2013;My skova´et al, 2014). aDNA analysis has, for example, been used to reclassify an egg finding, as shown by Cleeland et al (2013); they identified Physaloptera sp.…”

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confidence: 99%

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DNA Typing of Ancient Parasite Eggs from Environmental Samples Identifies Human and Animal Worm Infections in Viking-Age Settlement

Søe

Nejsum

Fredensborg

et al. 2015

Journal of Parasitology

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Ancient parasite eggs were recovered from environmental samples collected at a Viking-age settlement in Viborg, Denmark, dated 1018-1030 A.D. Morphological examination identified Ascaris sp., Trichuris sp., and Fasciola sp. eggs, but size and shape did not allow species identification. By carefully selecting genetic markers, PCR amplification and sequencing of ancient DNA (aDNA) isolates resulted in identification of: the human whipworm, Trichuris trichiura , using SSUrRNA sequence homology; Ascaris sp. with 100% homology to cox1 haplotype 07; and Fasciola hepatica using ITS1 sequence homology. The identification of T. trichiura eggs indicates that human fecal material is present and, hence, that the Ascaris sp. haplotype 07 was most likely a human variant in Viking-age Denmark. The location of the F. hepatica finding suggests that sheep or cattle are the most likely hosts. Further, we sequenced the Ascaris sp. 18S rRNA gene in recent isolates from humans and pigs of global distribution and show that this is not a suited marker for species-specific identification. Finally, we discuss ancient parasitism in Denmark and the implementation of aDNA analysis methods in paleoparasitological studies. We argue that when employing species-specific identification, soil samples offer excellent opportunities for studies of human parasite infections and of human and animal interactions of the past.

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Section: Morphological Examinationmentioning

confidence: 99%

Section: Paleoparasitologymentioning

confidence: 99%

mentioning

confidence: 99%

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DNA Typing of Ancient Parasite Eggs from Environmental Samples Identifies Human and Animal Worm Infections in Viking-Age Settlement

Søe

Nejsum

Fredensborg

et al. 2015

Journal of Parasitology

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“…Hawass et al 2010) and coprolites (e.g. Botella et al 2010), and sediments associated with human burials (e.g. Oh et al 2010 a ), have revealed a wealth of information about the parasites of prehistoric human populations.…”

Section: Applications and Insightsmentioning

confidence: 99%

DNA barcoding of ancient parasites

Wood

2018

Parasitology

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Ancient samples present a number of technical challenges for DNA barcoding, including damaged DNA with low endogenous copy number and short fragment lengths. Nevertheless, techniques are available to overcome these issues, and DNA barcoding has now been used to successfully recover parasite DNA from a wide variety of ancient substrates, including coprolites, cesspit sediment, mummified tissues, burial sediments and permafrost soils. The study of parasite DNA from ancient samples can provide a number of unique scientific insights, for example: (1) into the parasite communities and health of prehistoric human populations; (2) the ability to reconstruct the natural parasite faunas of rare or extinct host species, which has implications for conservation management and de-extinction; and (3) the ability to view in 'real-time' processes that may operate over century- or millenial-timescales, such as how parasites responded to past climate change events or how they co-evolved alongside their hosts. The application of DNA metabarcoding and high-throughput sequencing to ancient specimens has so far been limited, but in future promises great potential for gaining empirical data on poorly understood processes such as parasite co-extinction.

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“…80,81,83,84 Sequence alignment has revealed exact or near exact matches with sequences obtained from modern A. lumbricoides or A. suum but in most cases it has not been possible to unambiguously assign the archaeological samples to either species, partly due to the lack of a definitive diagnostic marker to distinguish between the two. The extraction of ancient DNA and amplification of Ascaris-specific markers has been carried out on a subset of more recent samples, which included isolated eggs and coprolites.…”

Section: Evolutionary History and Spread Of Ascarismentioning

confidence: 99%