Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

Oliveira, Angela de; Fonseca, Adivaldo Henrique da; Costa, C.; Mantovani, Elenice; Yoshinari, Natalino Hajime

doi:10.1590/s0074-02762010000500020

Cited by 11 publications

(10 citation statements)

References 13 publications

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“…Subsequently Preac-Mursic and colleagues demonstrated the formation and cultivation of B. burgdorferi ‘spheroplast-L-form variants’,78 and Brorson et al showed that these forms, which he termed cysts or round bodies, could revert to viable spiral forms of the bacteria 79,80. This observation has been confirmed by other investigators 81–83. Although the pathogenicity of CWD borrelial forms has been questioned,3 recent studies have suggested a link between CWD borrelia and neurodegenerative diseases,84–86 and resistance of cystic forms to antibiotic therapy has been documented 87–90.…”

Section: Renewed Interest In Cell Wall Deficient Bacterial Formsmentioning

confidence: 80%

Lyme disease: the next decade

Stricker¹,

Johnson²

2011

IDR

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Although Lyme disease remains a controversial illness, recent events have created an unprecedented opportunity to make progress against this serious tick-borne infection. Evidence presented during the legally mandated review of the restrictive Lyme guidelines of the Infectious Diseases Society of America (IDSA) has confirmed the potential for persistent infection with the Lyme spirochete, Borrelia burgdorferi, as well as the complicating role of tick-borne coinfections such as Babesia, Anaplasma, Ehrlichia, and Bartonella species associated with failure of short-course antibiotic therapy. Furthermore, renewed interest in the role of cell wall-deficient (CWD) forms in chronic bacterial infection and progress in understanding the molecular mechanisms of biofilms has focused attention on these processes in chronic Lyme disease. Recognition of the importance of CWD forms and biofilms in persistent B. burgdorferi infection should stimulate pharmaceutical research into new antimicrobial agents that target these mechanisms of chronic infection with the Lyme spirochete. Concurrent clinical implementation of proteomic screening offers a chance to correct significant deficiencies in Lyme testing. Advances in these areas have the potential to revolutionize the diagnosis and treatment of Lyme disease in the coming decade.

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Section: Renewed Interest In Cell Wall Deficient Bacterial Formsmentioning

confidence: 80%

Lyme disease: the next decade

Stricker¹,

Johnson²

2011

IDR

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“…The vast majority of research about B. burgdorferi morphologic variants has been conducted only in laboratory settings. Most of these studies are limited to describing morphology of B. burgdorferi in culture [36][37][38][87][88][89][90][91][92][93][94][95][96]. Round morphologic variants have been shown to arise in a variety of laboratory culture conditions, including cultivation in ex vivo human tonsillar tissue and human cerebrospinal fluid.…”

Section: Discussionmentioning

confidence: 99%

A Systematic Review of Borrelia burgdorferi Morphologic Variants Does Not Support a Role in Chronic Lyme Disease

Lantos

Auwaerter

Wormser

2013

Clinical Infectious Diseases

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“…He notes that these cystic structures, which Bockenstedt et al, observed in their infected animals ( Figure 2 in their study [10]), have been described as a persister mechanism employed by many bacteria, including B. burgdorferi [16][17][18][19][20][21][22][23][24]. The study authors claim that these are not true cysts because they form too fast, appearing in minutes rather than hours or days.…”

Section: Reviewmentioning

confidence: 94%