Molecular characterization of adenoviruses from children presenting with acute respiratory disease in Uberlândia, Minas Gerais, Brazil, and detection of an isolate genetically related to feline adenovirus

Luiz, Lysa Nepomuceno; Leite, José Paulo Gagliardi; Yokosawa, Jonny; Carneiro, Bruno Moreira; Filho, Edson Pereira; Oliveira, Thelma Fátima de Mattos Silva; Freitas, Guilherme Ramos Oliveira e; Costa, L. C.; Paula, Nayhanne Tizzo de; Silveira, Hélio Lopes da; Nepomuceno, Júlio César; Queiróz, Divina Aparecida Oliveira

doi:10.1590/s0074-02762010000500019

Cited by 24 publications

(21 citation statements)

References 32 publications

(38 reference statements)

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“…The presence of low-level antibodies to human AdVs in cats has been described in connection with vaccination trials using recombinant HAdV-5 as gene-delivery vector (Gonin, 1995). Our results indicate that, apart from HAdVs from the species Human mastadenovirus C (Lakatos et al, 1999b;Phan et al, 2006;Luiz et al, 2010), perhaps a distinct putative feline AdV also infects cats. Although the overall amount of the studied genomic sequences of the putative cat AdV is scarce, their base composition biased towards high A+T content allows for hypothesising a host switch event, as the existence of such a connection has been proposed before (Benkő and Harrach, 2003;Wellehan et al, 2004).…”

Section: Discussionsupporting

confidence: 61%

“…The sequence was identified as originating from HAdV-1 later, when the full genomic sequence of HAdV-1 was published (Lauer et al, 2004). Subsequently, the presence of HAdV-1 was confirmed in cats in Japan (Phan et al, 2006) and Brazil as well (Luiz et al, 2010), but no other reports on sequencelevel identification of AdVs in felid hosts have been published to date.…”

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confidence: 99%

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Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

Lakatos

Hornyák²,

Demeter

et al. 2017

Acta Veterinaria Hungarica

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Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in formalin-fixed paraffin-embedded tissue samples of a cat that had suffered from disseminated adenovirus infection. The identity of the amplified products from the hexon and DNA-dependent DNA polymerase genes was confirmed by DNA sequencing. The sequences were clearly distinguishable from corresponding hexon and polymerase sequences of other mastadenoviruses, including human adenoviruses. These results suggest the possible existence of a distinct feline adenovirus.

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Section: Discussionsupporting

confidence: 61%

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confidence: 99%

Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

Lakatos

Hornyák²,

Demeter

et al. 2017

Acta Veterinaria Hungarica

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“…Prevalent serotype in Brazil was Ad-4, and several parts of the world including Taiwan (Ad-3), USA (Ad-14, Ad-3 and Ad-7), and Canada (Ad-3, Ad-2, Ad-1 and Ad-21, respectively) [17] [18] [19] [20] [21].…”

Section: Discussionmentioning

confidence: 99%

Adenoviruses Type D in Patients with Respiratory Tract Infection Symptoms

Javid¹,

Moradi²,

Mohebbi³

et al. 2017

OALib

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Different Human Adenoviruses (HAdV) serotypes are associated with epidemic respiratory infections. The aim of this study was to detect HAdV from patients with respiratory symptoms and typing virus circulating in North of IRAN. Four hundred nasopharyngeal aspirate samples were obtained from patients with respiratory symptoms. Clinical data were collected. After DNA extraction, Polymerase Chain Reaction (PCR) was done for conserved Hexon region. Positive PCR products were used for sequencing grouping. 37 cases (9.2%) were positives. Clinical symptoms as cough (73%), body (67/6%), and fever (64/9%) were found in 35 infected patients (94/5%) and two patients (15/5%) had no symptoms. All samples were belonged to HAdV of species D (HAdV-8). Prevalence of respiratory adenovirus infection in our area is consistent with other studies in Iran and ocular type of HAdV8 was the frequent adenovirus serotype circulating in acute respiratory disease in Golestan province.

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“…Molecular methods demonstrate greater sensitivity than conventional assays for detecting adenovirus in respiratory samples 11,12 . Brazilian studies have described HAdV frequencies of 3 to 7.1%, as measured using different diagnostic techniques [13][14][15][16] . Outbreaks have been frequently reported among young patients 17 .…”

Section: Human Adenovirus Detection Among Immunocompetent and Immunocmentioning

confidence: 99%

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Watanabe

Carraro²,

Camargo

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction: Human adenoviruses (HAdV) play an important role in the aetiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was to detect HAdV using two different methods, direct fluorescence assay (DFA) and nested polymerase chain reaction (nested PCR), in samples collected from patients with acute respiratory infection (ARI) within 7 days of symptom onset. Methods: Samples (n=643) were collected from patients in different risk groups from 2001 to 2010: 139 adult emergency room patients (ERP); 205 health care workers (HCW); 69 renal transplant outpatients (RTO); and 230 patients in a haematopoietic stem cell transplantation program (HSCT). Results: Adenovirus was detected in 13.2% of the 643 patients tested by DFA and/or PCR: 6/139 (4.3%) adults in the ERP group, 7/205 (3.4%) in the HCW group, 4/69 (5.8%) in the RTO group and 68/230 (29.5%) in the HSCT patient group. Nested PCR had a higher detection rate (10%) compared with the DFA test (3.8%) (p<0.001). HSCT patients exhibited a significantly higher rate of HAdV infection. Conclusions: The adenovirus detection rate of the nested PCR assay was higher than that of the DFA test. However, the use of molecular methods in routine diagnostic laboratory work should be evaluated based on the specific circumstances of individual health services.

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Molecular characterization of adenoviruses from children presenting with acute respiratory disease in Uberlândia, Minas Gerais, Brazil, and detection of an isolate genetically related to feline adenovirus

Cited by 24 publications

References 32 publications

Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

Adenoviruses Type D in Patients with Respiratory Tract Infection Symptoms

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

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