Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats

Marra, Nelson Mendes; Chiuso-Minicucci, Fernanda; Machado, Gabriel Capella; Zorzella-Pezavento, Sofia Fernanda Gonçalves; França, Thaís Graziela Donegá; Ishikawa, Larissa Lumi Watanabe; Amarante, Alessandro Francisco Talamini do; Sartori, Alexandrina; Amarante, Mônica Regina Vendrame

doi:10.1590/s0074-02762010000100008

Cited by 23 publications

(33 citation statements)

References 26 publications

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“…21 In the clinical study, 54 samples were positive for S. stercoralis with the Baermann technique and duplicate stool cultures. 10 Of the positive samples 45 (83%) were positive by real-time PCR.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool

Sultana

Jeoffreys²,

Watts³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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Abstract. The use of real-time polymerase chain reaction (PCR) for detection of Strongyloides stercoralis in stool has recently been described. We compared five DNA extraction methods by using normal human stool spiked with Strongyloides ratti and tested by using a real-time PCR. The PowerSoil kit was found to be the best technique in terms of sensitivity and ease of use. The PCR detected DNA extracted from one spiked S. ratti larva diluted 10 −2 . The PowerSoil kit was then used to extract DNA from 160 human survey samples. All culture positive specimens with a high and moderate larval load were identified by real-time PCR, but only 15% of specimens with low larval load were positive. Specificity was greater than 99%. The combination of the PowerSoil kit and real-time PCR reliably detected high to moderate larval numbers of S. stercoralis in stools but was less sensitive when the larval load was low.

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Section: Discussionmentioning

confidence: 99%

Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool

Sultana

Jeoffreys²,

Watts³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

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“…Various polymerase chain reaction methods showed better sensitivity, but have lower sensitivity as burden of larvae decreases. 16 There are proposals for follow-up of antibody level after treatment, 17,18 however further research is needed to assess the value of serology as a marker of eradication.…”

Section: Discussionmentioning

confidence: 99%

Five Cases of Recurrent Meningitis Associated with Chronic Strongyloidiasis

Shimasaki¹,

Chung²,

Shiiki³

2015

The American Journal of Tropical Medicine and Hygiene

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Abstract. Although meningitis secondary to chronic strongyloidiasis is a rare complication, it is associated with a high mortality rate. Recurrent meningitis can occur if the underlying parasitic infection is left untreated. We report five cases of recurrent meningitis related to chronic strongyloidiasis that were associated with human T-lymphotropic virus type 1 (HTLV-1) infection. Common causative organisms are Escherichia coli, Streptococcus bovis, and Klebsiella pneumonia. One patient died during the second episode of meningitis. Three patients showed significant gastrointestinal and respiratory symptoms before developing headache and fever. In four cases, patients developed multiple recurrences even with the treatment of thiabendazol. Ivermectin seems to be a better agent compared with thiabendazol to achieve eradication of strongyloidiasis.

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“…the 18S rRNA small subunit (SSU); the internal transcribed spacer region 1 (ITS-1); the 28S rRNA gene; or the cyclooxygenase gene (cox1) 19,[23][24][25][26][27][28][29][30][32][33][34][35][36][37] . Published sensitivities and specificities for…”

Section: Pcrmentioning

confidence: 99%

The laboratory diagnosis of Strongyloides stercoralis

Watts¹,

Robertson²,

Bradbury

2016

Microbiol. Aust.

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It is estimated that over 30million people worldwide are infected by the nematode, Strongyloides stercoralis1. It is endemic in sub-tropical and tropical parts of Australia, with high rates of infection documented in some indigenous communities2. Due to the potential for chronic autoinfection, that may persist for decades, migration leads to the presence of the infection in non-endemic areas1. Transmission to humans is generally through the penetration of larvae through the skin, following contact with faecally contaminated soil1. Disease severity ranges from asymptomatic chronic carriage to an overwhelming illness, where large numbers spread throughout the body, usually triggered by immunosuppression1.

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Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats

Cited by 23 publications

References 26 publications

Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool

Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool

Five Cases of Recurrent Meningitis Associated with Chronic Strongyloidiasis

The laboratory diagnosis of Strongyloides stercoralis

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