Molecular mechanisms of Trypanosoma cruzi infection by oral route

Yoshida, Nobuko

doi:10.1590/s0074-02762009000900015

Cited by 81 publications

(51 citation statements)

References 39 publications

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“…Aunque sus antecedentes se remontan a la primera mitad del siglo XX, la transmisión oral es una forma de infección que ha recibido atención especialmente en la última década en varias regiones de América Latina, sobre todo en la Amazonia (14).…”

Section: Transmisión Por Vía Oralunclassified

Transmisión oral de Trypanosoma cruzi: un nuevo escenario epidemiológico de la enfermedad de Chagas en Colombia y otros países suramericanos

et al. 2014

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Section: Transmisión Por Vía Oralunclassified

Transmisión oral de Trypanosoma cruzi: un nuevo escenario epidemiológico de la enfermedad de Chagas en Colombia y otros países suramericanos

et al. 2014

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“…In the northeast region, the State of Bahia holds the highest level of human infection, with seroprevalence rates varying from 5.4 to 25.0% (CAMARGO et al, 1984;ARAS et al, 2002). The main form of contamination is by the feces of contaminated hematophagous triatomine arthropods (ROSYPAL et al, 2007) however, contamination through experimental breast-feeding (MEDINA LOPES, 1988), blood transfusion , and oral ingestion (YOSHIDA, 2009;SHIKANAI-YASUDA;CARVALHO, 2012) have been reported. Sylvatic and domestic animals play an important role in the life cycle of this parasite.…”

Section: Introductionmentioning

confidence: 99%

First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

Júnior

Almeida

Carvalho

et al. 2013

Rev. Bras. Parasitol. Vet.

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In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.Keywords: Trypanosoma cruzi, dog, PCR, Polymerase Chain Reaction, Bahia. ResumoCom o objetivo de verificar a infecção por Trypanosoma cruzi em cães domésticos domiciliados em área rural e endêmica do sul da Bahia, foi realizada a Reação em Cadeia da Polimerase (PCR), utilizando-se os iniciadores S35 e S36 em 272 cães domiciliados no distrito da Vila Operária, cidade de Buerarema. Todos os animais foram avaliados clinicamente e, posteriormente, foram coletados 2,5 mL de sangue por punção venosa para realização do diagnóstico molecular. Nenhum dos animais apresentou manifestação clínica da doença e, em apenas dois foram identificados DNA do parasito. Esse resultado é o primeiro relato de infecção natural por T. cruzi em cães domésticos no sul baiano.

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“…It is estimated that 8 million people are currently infected and that 90 million individuals living in areas of endemicity are at risk of infection (27,32). The parasite is transmitted to humans through the feces of infected, blood-sucking triatomine bugs, blood transfusion, organ transplantation, or congenital contamination or by the ingestion of tainted food and fluids (35). In recent years, several efforts have been successfully undertaken to control vectorial transmission in Latin American countries, with a concomitant decrease in the actual numbers of acute infections (28).…”

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Evaluation of a Recombinant Trypanosoma cruzi Mucin-Like Antigen for Serodiagnosis of Chagas' Disease

Marchi

Noia

Frasch

et al. 2011

Clin Vaccine Immunol

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Chagas' disease is caused by the protozoan parasite Trypanosoma cruzi and is one of the most important endemic problems in Latin America. Lately, it has also become a health concern in the United States and Europe. Currently, a diagnosis of Chagas' disease and the screening of blood supplies for antiparasite antibodies are achieved by conventional serological tests that show substantial variation in the reproducibility and reliability of their results. In addition, the specificity of these assays is curtailed by antigenic crossreactivity with sera from patients affected by other endemic diseases, such as leishmaniasis. Here we used a highly sensitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) to evaluate a recombinant protein core of a mucin-like molecule (termed trypomastigote small surface antigen [TSSA]) for the detection of specific serum antibodies in a broad panel of human sera. The same samples were evaluated by CL-ELISA using as the antigen either a mixture of native T. cruzi trypomastigote mucins or an epimastigote extract and, for further comparison, by conventional serologic tests, such as an indirect hemagglutination assay and indirect immunofluorescence assay. TSSA showed ϳ87% sensitivity among the seropositive Chagasic panel, a value which was increased up to >98% when only parasitologically positive samples were considered. More importantly, TSSA showed a significant increase in specificity (97.4%) compared to those of currently used assays, which averaged 80 to 90%. Overall, our data demonstrate that recombinant TSSA may be a useful antigen for the immunodiagnosis of Chagas' disease.

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Molecular mechanisms of Trypanosoma cruzi infection by oral route

Cited by 81 publications

References 39 publications

Transmisión oral de Trypanosoma cruzi: un nuevo escenario epidemiológico de la enfermedad de Chagas en Colombia y otros países suramericanos

Transmisión oral de Trypanosoma cruzi: un nuevo escenario epidemiológico de la enfermedad de Chagas en Colombia y otros países suramericanos

First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

Evaluation of a Recombinant Trypanosoma cruzi Mucin-Like Antigen for Serodiagnosis of Chagas' Disease

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