Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis

Ramos, Carlos A. N.; Araújo, Flábio R.; Souza, Ingrid I. F.; Oliveira, Renato; Elisei, Carina; Soares, Cleber Oliveira; Sacco, A. M. S.; Rosinha, G. M. S.; Alves, Lêucio Câmara

doi:10.1590/s0074-02762009000700010

Cited by 6 publications

(7 citation statements)

References 30 publications

(29 reference statements)

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“…The research and development of subunit vaccines have reached significant levels in recent years, including encouraging results, as in the case of protozoan Leishmania chagasi a GenBank accession numbers previously published by this research group in Ramos et al (2011). b GenBank accession numbers previously published by this research group in Ramos et al (2009). (Fernandes et al, 2008) and Theileria parva (Musoke et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the acs-1 protein, which demonstrated a high degree of genetic conservation between the Brazilian and American strains, induced the proliferation of TCD4+ memory cells and the production of INF-␥ in cattle chronically infected with B. bovis (Norimine et al, 2006), all of which are components of the Th1 protective immune response against B. bovis (Brown et al, 2006). It has recently been shown that recombinant ribosomal protein p0 from B. bovis was recognised by IgG1 and IgG2 antibodies from cattle chronically infected with B. bovis (Ramos et al, 2009). Moreover, acs-1 and p0 have been associated with protective immune responses against Plasmodium falciparum (Rajeshwari et al, 2004;Norimine et al, 2006), which is an organism related to B. bovis.…”

Section: Resultsmentioning

confidence: 99%

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Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Ramos

Araújo

Alves

et al. 2012

Veterinary Parasitology

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Bovine babesiosis caused by Babesia bovis remains an important constraint for the development of cattle industries worldwide. Effective control can be achieved by vaccination with live attenuated phenotypes of the parasite. However, these vaccines have a number of drawbacks, which justifies the search for better, safer vaccines. In recent years, a number of parasite proteins with immunogenic potential have been discovered. However, there is little information on the genetic conservation of these proteins among different parasite isolates, which hinders their assessment as immunogens. The aim of the present study was to evaluate the conservation of the genes ama-1, acs-1, rap-1, trap, p0 and msa2c among five Brazilian isolates of B. bovis. Through polymerase chain reaction, genetic sequencing and bioinformatics analysis of the genes, a high degree of conservation (98-100%) was found among Brazilian isolates of B. bovis and the T2Bo isolate. Thus, these genes are worth considering as viable candidates to be included in a recombinant cocktail vaccine for cattle babesiosis caused by B. bovis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Ramos

Araújo

Alves

et al. 2012

Veterinary Parasitology

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“…It has been shown that this surface protein contains erythrocyte binding domains participating in initial recognition and/or attachment events, and Rajeshwari et al (2004) demonstrated that a P. falciparum P0 peptide has been protective against challenge in a mice model (Arevalo-Pinzon et al 2010). Babesia P0 proteins show a high degree of inter-species cross-reactivity, and a Brazilian study showed that most cattle naturally infected with B. bovis or B. bigemina displayed antibodies against this protein (Ramos et al 2009). Based on these observations and the finding that antibodies against B. gibsoni P0 inhibited in vitro growth of B. bovis , P0 has been proposed as a vaccine antigen against bovine babesiosis or even against babesiosis in general (Brown et al 2006 b ; Terkawi et al 2007; Ramos et al 2009).…”

Section: Puzzle Pieces For Novel Vaccinesmentioning

confidence: 99%

“…Babesia P0 proteins show a high degree of inter-species cross-reactivity, and a Brazilian study showed that most cattle naturally infected with B. bovis or B. bigemina displayed antibodies against this protein (Ramos et al 2009). Based on these observations and the finding that antibodies against B. gibsoni P0 inhibited in vitro growth of B. bovis , P0 has been proposed as a vaccine antigen against bovine babesiosis or even against babesiosis in general (Brown et al 2006 b ; Terkawi et al 2007; Ramos et al 2009).…”

Section: Puzzle Pieces For Novel Vaccinesmentioning

confidence: 99%

Vaccines against bovine babesiosis: where we are now and possible roads ahead

Florín-Christensen

Suárez

Rodríguez³

et al. 2014

Parasitology

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SUMMARY Bovine babesiosis caused by the tick-transmitted haemoprotozoans Babesia bovis, Babesia bigemina and Babesia divergens commonly results in substantial cattle morbidity and mortality in vast world areas. Although existing live vaccines confer protection, they have considerable disadvantages. Therefore, particularly in countries where large numbers of cattle are at risk, important research is directed towards improved vaccination strategies. Here a comprehensive overview of currently used live vaccines and of the status quo of experimental vaccine trials is presented. In addition, pertinent research fields potentially contributing to the development of novel non-live and/or live vaccines are discussed, including parasite antigens involved in host cell invasion and in pathogen-tick interactions, as well as the protective immunity against infection. The mining of available parasite genomes is continuously enlarging the array of potential vaccine candidates and, additionally, the recent development of a transfection tool for Babesia can significantly contribute to vaccine design. However, the complication and high cost of vaccination trials hinder Babesia vaccine research, and have so far seriously limited the systematic examination of antigen candidates and prevented an in-depth testing of formulations using different immunomodulators and antigen delivery systems.

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“…The sequence of SjMLP/hsp70 was analyzed using EXPASY (http://ca.expasy.org/tools/) and its deduced amino acid sequence was predicted. Using multiple sequence alignment, a phylogenetic tree of SjMLP/hsp70 and its homologues from other species was generated using MEGA 4.0 software (Ramos et al 2009). Characteristic amino acids sequences and domains within the SjMLP/hsp70 protein were identified by their homology to previously characterized HSP70s from other species using ClustalW 2.0 (Holmes and Bruno 1994).…”

Section: Bioinformatic Analysis Of Sjmlp/hsp70mentioning

confidence: 99%

Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/hsp70) as a member of the HSP70 family

et al. 2010

Parasitol Res

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Schistosomes are the causative agent of schistosomiasis. The 70-kDa heat-shock proteins (HSP70) are considered the predominant HSP family and play a key regulatory role in parasite development and pathogenesis. Based on the published sequences in Genbank/EMBL, an open-reading frame (ORF) encoding 653 amino acids (XP_002581385.1) and belonging to the Schistosoma HSP70 protein family with a molecular weight of 71.49 kDa was identified by bioinformatic analysis. Since the sequence shared 77% identity with the published full-length Homo sapiens HSP70 protein, it was named Schistosoma mortalin-like protein (MLP/Hsp70). Here, we report the molecular and functional characterization of the Schistosoma japonicum SjMLP/hsp70 as a member of the HSP70 family. The complete SjMLP/hsp70 coding sequence was amplified from a S. japonicum adult worm cDNA library by polymerase chain reaction (PCR) and subcloned into the pET28a expression vector. The purified recombinant protein, rSjMLP/hsp70, was identified as a member of 70-kDa HSP family by mass spectrometry and could be recognized by the S. japonicum-infected mouse serum. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blotting analysis revealed that SjMLP/hsp70 was widely expressed in the eggs, cercariae, schistosomula, and adult worms of S. japonicum. A thermotolerance assay showed that rSjMLP/hsp70 could protect Escherichia coli cells from heat damage. This chaperone-like activity was demonstrated by full-length SjMLP/hsp70. The detection of specific antibody levels by indirect enzyme-linked immunosorbent assay and IFN-gamma secretion of splenocytes by ELISpot assay suggested that mice immunized with SjMLP/hsp70 were able to elicit Th1-type bias immune response. The challenge-protective experiment showed that DNA vaccine of SjGST combined with SjMLP/hsp70 could induce a 31.31% reduction of worm burden and 58.59% reduction of egg burden in intestinal tissue of immunized mice. Our results imply that SjMLP/hsp70 has a potential adjuvant function and might be a vaccine candidate for schistosomiaisis, which is the first report of the expression and preliminary characterization analysis of this molecule.

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Molecular and antigenic characterisation of ribosomal phosphoprotein P0 from Babesia bovis

Cited by 6 publications

References 30 publications

Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Vaccines against bovine babesiosis: where we are now and possible roads ahead

Molecular and functional characterization of a mortalin-like protein from Schistosoma japonicum (SjMLP/hsp70) as a member of the HSP70 family

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