Microbiological and host features associated with corynebacteriosis in cancer patients: a five-year study

Martins, Cas; Faria, Lmd; Souza, Monica Cristina de; Camello, Tcf; Velasco, Eduardo; Hirata, Raphael; Thuler, L.C.S.; Al, Mattos-Guaraldi

doi:10.1590/s0074-02762009000600015

Cited by 35 publications

(39 citation statements)

References 34 publications

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“…This observation is confirmed by the disturbing news about the discovery of a multidrug-resistant clone of C. striatum in Italy, which was isolated from infections in long-term inpatients [5]. In agreement with this study, its contribution to infections of the blood [36], wounds [35,37] and the respiratory tract [38] was confirmed. The case reports indicate the special incidence of this type of infection in long-term hospitalized patients.…”

Section: Resultssupporting

confidence: 80%

Antibiotic Resistance and Detection of the Most Common Mechanism of Resistance (MLSB) of Opportunistic Corynebacterium

Olender¹

2013

Chemotherapy

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Background: Determination of antibiotic resistance of opportunistic Corynebacterium colonizing the nose that cause infections and evaluation of the applicability of a simple method for detecting the most common constitutive-type resistance to macrolides, lincosamides and streptogramin B (MLSB). Methods: 70 isolates colonizing the nose and 70 clinical isolates of various infection sites were used and identified using APICoryne and 16S rRNA. Minimal inhibitory concentrations (MICs) were determined (Etest) for 12 antibiotics. MLSB was defined based on MIC, a simple method using two disks (erythromycin/clindamycin) and detection of the gene erm X (PCR). Results: There was a high percentage - in both groups at the same level - of strains with MLSB (88.5% colonizing the nose and 87.1% causing infections). Detection with the phenotypic method MLSB was confirmed genetically (erm X) in all cases. In both groups, a high percentage of resistance was found to trimethoprim/sulfamethoxazole (in both groups 71.4%), chloramphenicol (nose 44.2%/infections 37.1%), tetracycline (28 and 45.7%) and β-lactam antibiotics (18.5 and up to 32.8%). Conclusion: Differences in antibiotic resistance were found between strains colonizing the respiratory tract and various infections. Isolates from infections more frequently exhibited multidrug resistance. The possibility of using a simple method was confirmed for MLSB detection, which can be applied to determine drug resistance in routine microbiological diagnostics of infections caused by opportunistic Corynebacterium.

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Section: Resultssupporting

confidence: 80%

Antibiotic Resistance and Detection of the Most Common Mechanism of Resistance (MLSB) of Opportunistic Corynebacterium

Olender¹

2013

Chemotherapy

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“…All these Corynebacterium spp. can cause invasive disease in patients with compromised skin or mucosal barrier function (e.g., patients suffering from burn wounds or carrying intravascular devices) or in immunocompromised patients (e.g., diabetics and cancer patients) (7)(8)(9)(10)(11)(12)(13).…”

mentioning

confidence: 99%

Toll-Like Receptor 2 and Mincle Cooperatively Sense Corynebacterial Cell Wall Glycolipids

et al. 2017

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Nontoxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans cause invasive disease in humans and animals. Host sensing of corynebacteria is largely uncharacterized, albeit the recognition of lipoglycans by Toll-like receptor 2 (TLR2) appears to be important for macrophage activation by corynebacteria. The members of the order Corynebacterineae (e.g., mycobacteria, nocardia, and rhodococci) share a glycolipid-rich cell wall dominated by mycolic acids (termed corynomycolic acids in corynebacteria). The mycolic acid-containing cord factor of mycobacteria, trehalose dimycolate, activates the C-type lectin receptor (CLR) Mincle. Here, we show that glycolipid extracts from the cell walls of several pathogenic and nonpathogenic Corynebacterium strains directly bound to recombinant Mincle in vitro. Macrophages deficient in Mincle or its adapter protein Fc receptor gamma chain (FcR␥) produced severely reduced amounts of granulocyte colony-stimulating factor (G-CSF) and of nitric oxide (NO) upon challenge with corynebacterial glycolipids. Consistently, cell wall extracts of a particular C. diphtheriae strain (DSM43989) lacking mycolic acid esters neither bound Mincle nor activated macrophages. Furthermore, TLR2 but not TLR4 was critical for sensing of cell wall extracts and whole corynebacteria. The upregulation of Mincle expression upon encountering corynebacteria required TLR2. Thus, macrophage activation by the corynebacterial cell wall relies on TLR2-driven robust Mincle expression and the cooperative action of both receptors.KEYWORDS C-type lectin receptor, Mincle, Toll-like receptor, macrophage, Corynebacterium diphtheriae, cell wall lipids, mycolate, mycolic acid, corynomycolate D iphtheria caused by toxin-producing Corynebacterium diphtheriae is a severe, life-threatening infection, which has become rare in Europe due to the efficacy and coverage of toxoid immunization but still causes a considerable burden of disease globally. Corynebacterium ulcerans and Corynebacterium pseudotuberculosis can also harbor the tox gene encoding diphtheria toxin (1) and in addition may secrete the exotoxin phospholipase D, a virulence factor involved in caseous lymphadenitis of sheep and goats (2, 3). Rates of infections with nontoxigenic strains of C. diphtheriae, including bloodstream infections and endocarditis, appear to be increasing in Europe (4,5). Of the 90 species that comprise the genus Corynebacterium, many inhabit the human skin or mucosa as commensals (6), with C. striatum, C. tuberculostearicum, C. amycolatum, and C. jeikeium being typical examples of skin inhabitants, whereas C. urealyticum and C. glucuronolyticum are frequently found in the urogenital tract. All

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“…Corynebacterium striatum is an emerging multidrug-resistant (MDR) potentially pathogenic microorganism that causes nosocomial infection in patients who have experienced long hospital admissions, those who have received several courses of antibiotics ( Camello et al 2003 , Otsuka et al 2006 , Renom et al 2007 , Baio et al 2013 ), those with acquired immune deficiency syndrome (AIDS) or cancer and those who have received a transplant ( Tarr et al 2003 , Martins et al 2009 ). Cases of severe infections in both immunocompromised and immunocompetent individuals and nosocomial outbreaks due to MDR C. striatum are increasing in both industrialised and developing countries.…”

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confidence: 99%

Biofilm production by multiresistant Corynebacterium striatumassociated with nosocomial outbreak

Souza

Faria

Sant’Anna

et al. 2015

Mem. Inst. Oswaldo Cruz

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Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbreaks. However, little is known about its virulence factors that may contribute to healthcare-associated infections (HAIs). We investigated the biofilm production on abiotic surfaces of multidrug-resistant (MDR) and multidrug-susceptible (MDS) strains of C. striatum of pulsed-field gel electrophoresis types I-MDR, II-MDR, III-MDS and IV-MDS isolated during a nosocomial outbreak in Rio de Janeiro, Brazil. The results showed that C. striatum was able to adhere to hydrophilic and hydrophobic abiotic surfaces. The C. striatum 1987/I-MDR strain, predominantly isolated from patients undergoing endotracheal intubation procedures, showed the greatest ability to adhere to all surfaces. C. striatum bound fibrinogen to its surface, which contributed to biofilm formation. Scanning electron microscopy showed the production of mature biofilms on polyurethane catheters by all pulsotypes. In conclusion, biofilm production may contribute to the establishment of HAIs caused by C. striatum.

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Microbiological and host features associated with corynebacteriosis in cancer patients: a five-year study

Cited by 35 publications

References 34 publications

Antibiotic Resistance and Detection of the Most Common Mechanism of Resistance (MLSB) of Opportunistic Corynebacterium

Antibiotic Resistance and Detection of the Most Common Mechanism of Resistance (MLSB) of Opportunistic Corynebacterium

Toll-Like Receptor 2 and Mincle Cooperatively Sense Corynebacterial Cell Wall Glycolipids

Biofilm production by multiresistant Corynebacterium striatumassociated with nosocomial outbreak

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