Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey

Doğruman-Al, Funda; Kuştımur, Semra; Yoshikawa, Hideki; Tuncer, Candan; Simsek, Zahide; Tanyüksel, Mehmet; Araz, Engin; Boorom, Kenneth

doi:10.1590/s0074-02762009000500011

Cited by 115 publications

(76 citation statements)

References 25 publications

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“…In this study, the pathogenesis of B. hominis subtypes is not investigated but some differences in virulence between Blastocystis subtypes was shown (9,20,31,32). In many studies, pathogenic potential of subtype 3 has been reported (16,31,(33)(34)(35). Also, some investigations have demonstrated that other subtypes such as subtype 1 were associated with symptoms (20,31,34,36).…”

Section: Discussionmentioning

confidence: 85%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

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Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. The parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B. hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background:Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the Blastocystis hominis subtypes and their prevalence rates in Hamadan. Materials and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1 % frequency. The prevalence rate of genotype SB227 and SB340 were 22 % and 7.3 %, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6 % of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

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Section: Discussionmentioning

confidence: 85%

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Sardarian

Hajilooi

Maghsood

et al. 2012

Jundishapur J Microbiol

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“…In numerous other investigations there was no association between Blastocystis subtypes and gastrointestinal symptoms [31,51,52]. They identified ST3 as the most frequent subtype in both groups (symptomatic and asymptomatic), but did not observe a significant relationship between this subtype and gastrointestinal symptoms.…”

Section: Pathogenicity Of Blastocystis Sp Is a Relationship Possiblementioning

confidence: 85%

Genetic diversity and pathogenicity of Blastocystis

Skotarczak¹

2018

Ann Agric Environ Med.

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Blastocystis is a unicellular, anaerobic protist which lives in the intestinal tract of diverse animals, including humans. It was found that the host specificity and the pathogenic potential of different isolates are correlated with sequence variations in the SSU-rRNA gene. Identification of the organism to the species level is still an unresolved challenge. Genetic diversity revisions have led to the identification of 17 subtypes (STs) within the Blastocystis genus, and 9 (ST1 to ST9) have been reported in humans with varying prevalence. Since the members of the genus revealed a large genetic diversity, several molecular modalities of subtyping methods have been developed. Numerous studies on conveying the pathogenic potential to the molecular subtypes are available, but they could not be compared or analysed with the different molecular techniques employed. The use of different approaches may give false positives during diagnosis and the possibility of missed infections. A review of recent scientific literature indicates that the development of PCR assays is needed for molecular epidemiology and for mixed infections in health and disease cohorts, and also to help identify sources of Blastocystis transmission to humans, as well as to identify potential animal and environmental reservoirs. This review summarizes some of the recent progress and improvements in Blastocystis research on genetic diversity, taxonomy, molecular epidemiology, pathogenicity and subtyping methods.

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“…Numbers of female and male participants were 143 (62.2%) and 87 (37.8%), respectively. Distribution of the individuals according to the age groups was nine (3.9%) for age 7-19, 76 (33%) for age 20-39, 90 (39.1%) for age [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59], and 55 (24%) for age 60 and above. A minority of the participants (24%) were university graduates; however, most of the individuals (91.7%) had middle or high socioeconomic status.…”

Section: Resultsmentioning

confidence: 99%

“…[6][7][8] In Turkey, the most predominant Blastocystis ST was documented as ST3, which was followed by ST1 and ST2. 12,50,51 In the European countries, distribution of the STs tended to be similar to Turkey, and in addition, ST4 was commonly observed. 8 On the contrary, ST5-ST9 were detected sporadically in humans.…”

Section: Discussionmentioning

confidence: 95%

Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

Seyer¹,

Karasartova²,

Ruh³

et al. 2017

Am J Trop Med Hyg

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Abstract. This study was conducted to investigate the prevalence of Blastocystis spp. and its subtypes (STs) in North Cyprus; and to evaluate the presence of this parasite and its STs with respect to demographic, socioeconomic, and epidemiological factors, as well as gastrointestinal symptoms. Stool samples were collected from 230 volunteers. Each participant also filled out a questionnaire. The samples were examined microscopically by native-Lugol and trichrome methods and further tested by polymerase chain reaction (PCR) and sequencing. Prevalence of Blastocystis spp. infection was found to be 10.5%, 10.5%, and 27.8%, by direct microscopy, trichrome method, and PCR, respectively. No other parasites were detected in the specimens except Giardia spp. (n = 2; 0.8%) and Entamoeba coli (n = 1; 0.4%). The most common Blastocystis STs were ST3 (20; 31.2%), ST2 (18; 28.2%), ST1 (8; 12.5%), and ST4 (7; 11%); whereas other STs were identified as ST6 (3; 4.7%), ST7 (2; 3.2%), and non-ST (6; 9.4%). Presence of Blastocystis spp. and its STs was not significantly related to any of the demographic, socioeconomic, and epidemiological factors. Furthermore, no significant association of Blastocystis spp. and its STs with gastrointestinal symptoms was found. This study is the first investigation of the epidemiology of Blastocystis spp. in North Cyprus. Distribution of Blastocystis spp. and its STs among demographic, socioeconomic, and epidemiological factors showed complete homogeneity. Presence of the parasite and its STs was not significantly related with the gastrointestinal symptoms among symptomatic and asymptomatic individuals. These findings suggest that Blastocystis spp. may be part of the intestinal flora in humans.

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Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey

Abstract: Blastocystis infection has been reported to be associated with irritable bowel syndrome (IBS)

Cited by 115 publications

References 25 publications

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

A Study of The Genetic Variability of Blastocystis hominis Isolates in Hamadan, West of Iran

Genetic diversity and pathogenicity of Blastocystis

Epidemiology and Prevalence of Blastocystis spp. in North Cyprus

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