Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae

Rocha, Abraham; Braga, Cynthia; Belém, Marcela; Carrera, Arturo; Aguiar-Santos, Ana Maria; Oliveira, Paula; Texeira, Maria José; Furtado, André Freire

doi:10.1590/s0074-02762009000400015

Cited by 30 publications

(31 citation statements)

References 27 publications

(29 reference statements)

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“…The comparative evaluation of the performance of the thick fi lm test in relation to the AD12-ICT card test using the Hayen et al 28 formula also showed that in each group of 1,000 schoolchildren with filarial infection, the AD12-ICT card test was able to detect approximately 150 more cases than the thick fi lm test. These results are in agreement with previous studies conducted in the school-age population [29][30][31][32][33][34][35][36][37][38][39] and reinforce the usefulness of the AD12-ICT card test in screening for fi larial infection in children for the assessment of MDA effi cacy 6,40 . The greater sensitivity of the antigen tests can be explained by their ability to detect low-level microfi laremia and amicrofi laremic cases that are typically more common in children than in adults 7 .…”

Section: Discussionsupporting

confidence: 92%

Evaluation of diagnostic tests for Wuchereria bancrofti infection in Brazilian schoolchildren

Oliveira

Braga

Alexander

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Introduction:Since the launch of the Global Programme to Eliminate Lymphatic Filariasis, more than 70% of the endemic countries have implemented mass drug administration (MDA) to interrupt disease transmission. The monitoring of fi larial infection in sentinel populations, particularly schoolchildren, is recommended to assess the impact of MDA. A key issue is choosing the appropriate tools for these initial assessments (to defi ne the best intervention) and for monitoring transmission. Methods: This study compared the pre-MDA performance of fi ve diagnostic methods, namely, thick fi lm test, Knott's technique, fi ltration, Og4C3-ELISA, and the AD12-ICT card test, in schoolchildren from Brazil. Venous and capillary blood samples were collected between 11 pm and 1 am. The microfi larial loads were analyzed with a negative binomial regression, and the prevalence and associated 95% confi dence intervals were estimated for all methods. The accuracies of the AD12-ICT card and Og4C3-ELISA tests were assessed against the combination of parasitological test results. Results: A total of 805 schoolchildren were examined. The overall and stratifi ed prevalence by age group and gender detected by Og4C3-ELISA and AD12-ICT were markedly higher than the prevalence estimated by the parasitological methods. The sensitivity of the AD12-ICT card and Og4C3-ELISA tests was approximately 100%, and the positive likelihood ratios were above 6. The specifi city of the Og4C3-ELISA was higher than that of the AD12-ICT at different prevalence levels. Conclusions: The ICT card test should be the recommended tool for monitoring school-age populations living in areas with ongoing or completed MDA.

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Section: Discussionsupporting

confidence: 92%

Evaluation of diagnostic tests for Wuchereria bancrofti infection in Brazilian schoolchildren

Oliveira

Braga

Alexander

et al. 2014

Rev. Soc. Bras. Med. Trop.

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“…This technique helps in detection of circulating filarial antigen (CFA) in serum and plasma, as well as in other body fluids. Studies have satisfactorily demonstrated the high sensitivity of the Og4C3 ELISA assay in positive individuals 3 6. Many studies have shown the specificity of Og4C3 testing to be around 95%.…”

Section: Discussionmentioning

confidence: 98%

“…Few studies have demonstrated the role of FNAC and biopsy in confirming the presence of this parasite. Ultrasonogram of the breast and mammography have been reported to detect the parasite as well 3. Inflammatory carcinoma of the breast is a rare, highly malignant and fatal form of breast cancer.…”

Section: Discussionmentioning

confidence: 99%

Breast filariasis or inflammatory breast carcinoma? Reaching a diagnosis

Panda¹,

Mohapatra

2015

BMJ Case Reports

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SUMMARYLymphatic filariasis is a neglected tropical disease caused by the parasite Wuchereria bancrofti. Involvement of the breast is relatively uncommon and may affect only 10% of individuals with lymphatic filariasis. We present a case of an elderly woman with breast pathology. The condition clinically appeared as a malignancy. Routine diagnostic tests including tissue histopathological studies failed to clinch a diagnosis. However, a serological test helped in identifying the pathology, which turned out to be a manifestation of breast filariasis, and aided in instituting appropriate therapy for this condition. BACKGROUND

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“…With the aid of the Og4C3 antigen ELISA using nocturnal blood collection as indicator for the infestation with adult worms among children in India, Bal et al [40] found a prevalence of 32%, while with the aid of microscopy the detection rate for microfilariemia was only 10%. Rocha et al [41] compared the sensitivity of 2 antigen tests (Og4C3-ELISA and AD12-ICT card test) for circulating antigen of W. bancrofti (samples collected at night time) with the ultrasound analysis for the detection of the infestation with adult worms. A sensitivity rate of 97% was reached by means of the antigen test and 73% with the aid of the ultrasound method.…”

Section: Current Knowledge About the Pathogenmentioning

confidence: 99%

Arbonematodes – Nematode Infections Transmissible by Arthropods

2013

Transfus Med Hemother

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Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae

Cited by 30 publications

References 27 publications

Evaluation of diagnostic tests for Wuchereria bancrofti infection in Brazilian schoolchildren

Evaluation of diagnostic tests for Wuchereria bancrofti infection in Brazilian schoolchildren

Breast filariasis or inflammatory breast carcinoma? Reaching a diagnosis

Arbonematodes – Nematode Infections Transmissible by Arthropods

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