Lesion aspirate culture for the diagnosis and isolation of Leishmania spp. from patients with cutaneous leishmaniasis

Genus-specific kinetoplast-DNA PCR and parasite culture for the diagnosis of localised cutaneous leishmaniasis: applications for clinical trials under field conditions in Brazil

“…The present research, which evaluated a substantially higher number of patients, did not confirm the findings of our previous reports, but the low receptivity of the patients allowing the lymph node puncture. The overall skin culture and PCR yield was similar to those reported by other authors (Luz et al 2009), which confirmed the reproducibility of this method. The low contamination rate was also reproduced in the present study with a larger number of observations.…”

“…These primers have been extensively used in Brazil for genus-specific diagnosis of Leishmania infection (Degrave et al 1994a, b). Authors have described those primers with different names and notations (Pirmez et al 1999, Romero et al 2001a, Volpini et al 2004, De Assis et al 2008, Luz et al 2009). The amplification procedure was performed in a Perkin Elmer GeneAmp PCR System 2400 thermocycler (The Perkin Elmer Corporation, Norwalk, CT, USA).…”

Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis

“…Patients with LCL, ML and DL present with positive results. Positivity in LCL patients varies and is approximately 82-89% [56][57][58][59][60]. In ML [58] and DL [19] patients' positivity is usually 100%.…”

Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre

“…Various studies have suggested the usefulness of molecular techniques for the aetiological diagnosis of ATL (Pirmez et al 1999, de Oliveira et al 2003, Schonian et al 2003, Garcia et al 2004, Marques et al 2006, Gomes et al 2008, Luz et al 2009). In different studies conducted on humans, the sensitivity of the polymerase chain reaction (PCR) was about 100%, whereas 60% was observed for other types of exams.…”