Molecular detection of human astrovirus in an urban sewage treatment plant in Rio de Janeiro, Brazil

Guimarães, Flávia Ramos; Ferreira, Fabiana Fioretti Martins; Vieira, Carmen Baur; Fumian, Túlio Machado; Shubo, Tatsuo; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira

doi:10.1590/s0074-02762008000800013

Cited by 19 publications

(10 citation statements)

References 31 publications

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“…In another study, the percentage of norovirus detected ranged from 15% to 58%, depending on the methodology of detection used (Victoria et al in press). Although we could not demonstrate the recovery efficiency of the adsorption elution method for JCPyV, previous studies in this STP showed a recovery efficiency of 4.2% and 4.3% for raw and treated sewage samples for human astrovirus, and 7.8% and 4.6% for norovirus, respectively (Guimaraes et al 2008;Victoria et al in press). This same method, when used to concentrate waste water samples, presented satisfactory results for detection of enteric viruses in STPs in other studies Katayama et al 2008).…”

Section: Resultscontrasting

confidence: 62%

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Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Fumian¹,

Guimarães

Vaz³

et al. 2010

Journal of Water and Health

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Polyomavirus JC (JCPyV) is largely excreted by the human population through the urinary route and has been recognized as a potential viral marker for human waste contamination. This study aims to investigate the dissemination of JCPyV in waste water from a sewage treatment plant (STP) located in Rio de Janeiro, Brazil, and to describe the prevalence of JCPyV subtypes currently present in this population. Raw and treated sewage samples were collected bimonthly during one year, and examined for the presence of JCPyV using nested polymerase chain reaction (nPCR) and quantitative real time PCR (qPCR). JCPyV was detected by nPCR in 96% and 43% of raw and treated sewage samples, respectively. The concentration of JCPyV present in the samples ranged from 1.2 £ 10 3 to 3.2 £ 10 5 and 2.6 £ 10 2 to 6.2 £ 10 3 genome copies per 2 ml of concentrated raw and treated sewage sample, respectively. The strains were characterized and the obtained nucleotide sequences indicated that the detected JCPyV strains clustered with subtypes of East African, West African and European origin. To our knowledge, this is the first study describing the incidence and diversity of JCPyV strains in raw and treated sewage in Brazil.

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Section: Resultscontrasting

confidence: 62%

“…From January to December 2005, a total of 47 sewage composite samples, 24 from raw and 23 from treated sewage, were collected twice a month from an activated sludge STP in the city of Rio de Janeiro, Brazil, as described by Guimaraes et al (2008). One sample from treated sewage was not in sufficient quantity to test for JCPyV.…”

Section: Sewage Samplesmentioning

confidence: 99%

Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Fumian¹,

Guimarães

Vaz³

et al. 2010

Journal of Water and Health

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“…Katayama et al (39) and Haramoto et al (25) developed a virus concentration method that consists of adding salts for adsorption of viruses to a negatively charged filter membrane and an acid rinse step, as well as inorganic eluent, to remove PCR inhibitors. This virus filtration and concentration method was modified and used in several recent studies, with various virus recovery efficiencies, ranging from Ͻ1% to Ͼ100% (13,21,22,26,52,67). In the adenovirus seeding experiment, virus recovery quantified by real-time PCR was low; mean recoveries from the MilliQ water and river water were 2.08% (range, 0.17 to 6.98%) and 0.98% (range, 0.32 and 1.47%), respectively.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Detection of Human Adenoviruses in Wastewater and Combined Sewer Overflows Influencing a Michigan River

Fong

Phanikumar

Xagoraraki

et al. 2010

Appl Environ Microbiol

209

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Enteric viruses are important pathogens found in contaminated surface waters and have previously been detected in waters of the Great Lakes. Human adenoviruses were monitored because of their high prevalence and persistence in aquatic environments. In this study, we quantified adenoviruses in wastewater, surface water, and combined sewer overflows (CSOs) by real-time PCR. Between August 2005 and August 2006, adenovirus concentrations in raw sewage, primary-treated effluent, secondary-treated effluent, and chlorinated effluent from a wastewater treatment plant in Michigan were examined. CSO samples (n ‫؍‬ 6) were collected from a CSO retention basin in Grand Rapids, MI. Adenoviruses were detected in 100% of wastewater and CSO discharge samples. Average adenovirus DNA concentrations in sewage and CSOs were 1.15 ؋ 10 6 viruses/liter and 5.35 ؋ 10 5 viruses/liter, respectively. Adenovirus removal was <2 log 10 (99%) at the wastewater treatment plant. Adenovirus type 41 (60% of clones), type 12 (29%), type 40 (3%), type 2 (3%), and type 3 (3%) were isolated from raw sewage and primary effluents (n ‫؍‬ 28). Six of 20 surface water samples from recreational parks at the lower Grand River showed virus concentrations above the real-time PCR detection limit (average, 7.8 ؋ 10 3 viruses/liter). This research demonstrates that wastewater effluents and wastewater-impacted surface waters in the lower Grand River in Michigan contain high levels of viruses and may not be suitable for full-body recreational activities. High concentrations of adenovirus in these waters may be due to inefficient removal during wastewater treatment and to the high persistence of these viruses in the environment.

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“…Products are visualized by agarose gel electrophoresis and can be confirmed by Southern blot hybridizations using an internal oligonucleotide probe or by sequencing the DNA product. This has been replaced for the most part by real-time PCR and real-time RT-PCR assays (Guimaraes et al 2008;Gutierrez-Aguirre et al 2008;Houde et al 2007;Jones et al 2009;Jothikumar et al 2005;Kageyama et al 2003;Kou et al 2008;Nordgren et al 2008;Scipioni et al 2008;Ward et al 2009;Wolf et al 2007). The real-time assays combine primer amplification with detection of the amplified product in a single reaction mix.…”

Section: Methods Of Detectionmentioning

confidence: 99%

Analytical Methods for Food and Environmental Viruses

Mattison

Bidawid

2009

Food Environ Virol

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There are many challenges for developing and selecting methods to detect enteric viruses from food and environmental samples. Growth methods are rarely available and the viruses have a low infectious dose, so methods must be very sensitive as well as specific. This review discusses methods for sample preparation, detection and typing, outlining strengths and weaknesses for different protocols. Enteric viruses are very stable in the environment and the development of effective detection methods is an important step towards reducing contamination of foods and the environment.

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Molecular detection of human astrovirus in an urban sewage treatment plant in Rio de Janeiro, Brazil

Abstract: The objective of this study was to evaluate the prevalence and dissemination of human astroviruses (HAstV)

Cited by 19 publications

References 31 publications

Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Molecular detection, quantification and characterization of human polyomavirus JC from waste water in Rio De Janeiro, Brazil

Quantitative Detection of Human Adenoviruses in Wastewater and Combined Sewer Overflows Influencing a Michigan River

Analytical Methods for Food and Environmental Viruses

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