Genetic divergence between two sympatric species of the Lutzomyia longipalpis complex in the paralytic gene, a locus associated with insecticide resistance and lovesong production

Lins, R. M. M. A.; Souza, Nataly A.; Peixoto, Alexandre A.

doi:10.1590/s0074-02762008000700019

Cited by 35 publications

(37 citation statements)

References 27 publications

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“…Our results also support the indings of a study by Lins et al (2008), who identi ied a clear difference between two populations of males with one and two pairs of spots collected in Sobral (CE), using the paralytic (para) gene. This gene encodes for a sodium channel associated with resistance to pyrethroid insecticides and production of courtship songs in Drosophila melanogaster (Meigen), as a molecular marker.…”

Section: Discussionsupporting

confidence: 90%

“…This gene encodes for a sodium channel associated with resistance to pyrethroid insecticides and production of courtship songs in Drosophila melanogaster (Meigen), as a molecular marker. Lins et al (2008) were the irst authors to demonstrate the association of the para gene with ixed differences between these two populations, raising the possibility that they may be associated with an incipient process of resistance to insecticides in these populations of L. longipalpis.…”

Section: Discussionmentioning

confidence: 99%

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Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males

Silva¹,

Nascimento²,

Leonardo³

et al. 2011

Neotrop. entomol.

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Entomological surveys in the state of Maranhão have recorded morphologically distinct populations of Lutzomyia longipalpis (Lutz & Neiva). Some populations have one pair of spots (1S) on the fourth tergite, while others have two pairs (2S) on the third and fourth tergites of males. In the present study we investigated the degree of genetic polymorphism among four populations in the municipalities of Caxias, Codó and Raposa, in the state of Maranhão, Brazil, by using RAPD (Random Ampli ied Polymorphic DNA) markers. A total of 35 loci were identi ied, of which 30 were polymorphic. The highest polymorphism was observed with primer OPA 4, which produced 11 different pro iles. Genetic diversity was assessed using grouping methods that produced a dendrogram in which the genotypes could be clearly separated into two main clades according to the number of spots on the male abdominal tergites. One cluster contained the populations from Caxias and Codó, and the other was formed by the populations from Raposa and Codó. The results of our RAPD analysis showed a clear separation between the populations with one and two pairs of spots. The epidemiologic signi icance of this genetic differentiation should be investigated in future studies.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males

Silva¹,

Nascimento²,

Leonardo³

et al. 2011

Neotrop. entomol.

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“…papatasi from Sudan [33], but without clear links to insecticide resistance. Sequencing of a fragment of the para gene in Lutzomyia longipalpis from Brazil detected non-synonymous variants, though the amino acid polymorphisms have not be linked with resistance in any previous studies [34,35]. In contrast, the three polymorphisms we identified in P .…”

Section: Discussioncontrasting

confidence: 71%

Knockdown resistance mutations predict DDT resistance and pyrethroid tolerance in the visceral leishmaniasis vector Phlebotomus argentipes

et al. 2017

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BackgroundIndoor residual spraying (IRS) with DDT has been the primary strategy for control of the visceral leishmaniasis (VL) vector Phlebotomus argentipes in India but efficacy may be compromised by resistance. Synthetic pyrethroids are now being introduced for IRS, but with a shared target site, the para voltage-gated sodium channel (VGSC), mutations affecting both insecticide classes could provide cross-resistance and represent a threat to sustainable IRS-based disease control.Methodology/Principal findingsA region of the Vgsc gene was sequenced in P. argentipes from the VL hotspot of Bihar, India. Two knockdown resistance (kdr) mutations were detected at codon 1014 (L1014F and L1014S), each common in mosquitoes, but previously unknown in phlebotomines. Both kdr mutations appear largely recessive, but as homozygotes (especially 1014F/F) or as 1014F/S heterozygotes exert a strong effect on DDT resistance, and significantly predict survivorship to class II pyrethroids in short-duration bioassays. The mutations are present at high frequency in wild P. argentipes populations from Bihar, with 1014F significantly more common in higher VL areas.Conclusions/SignificanceThe Vgsc mutations detected appear to be a primary mechanism underlying DDT resistance in P. argentipes and a contributory factor in reduced pyrethroid susceptibility, suggesting a potential impact if P. argentipes are subjected to suboptimal levels of pyrethroid exposure, or additional resistance mechanisms evolve. The assays to detect kdr frequency changes provide a sensitive, high-throughput monitoring tool to detecting spatial and temporal variation in resistance in P. argentipes.

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“…A total of 83 intraspecific studies has been recorded in the literature (Esseghir et al, 1997;Marcondes et al, 1997;Dias et al, 1998;Aransay et al, 2000Aransay et al, , 2003Di Muccio et al, 2000;Esseghir and Ready, 2000;Mukhopadhyay et al, 2000;Yin et al, 2000;Marquez et al, 2001;Soto et al, 2001;Arrivillaga et al, 2002Arrivillaga et al, , 2003Bauzer et al, 2002a,b;Depaquit et al, , 2004aDepaquit et al, , 2005bDepaquit et al, , 2008bDepaquit et al, , 2013Depaquit et al, , 2014Hodgkinson et al, 2002Hodgkinson et al, , 2003Testa et al, 2002;Bottecchia et al, 2004;Margonari et al, 2004;Pesson et al, 2004;Yahia et al, 2004;Elnaiem et al, 2005;Meneses et al, 2005;Perrotey et al, 2005;Dvorak et al, 2006Dvorak et al, , 2011Mazzoni et al, 2006Mazzoni et al, , 2008de Souza Rocha et al, 2007;Hamarsheh et al, 2007;Moin-Vaziri et al, 2007a,b;Baron et al, 2008;Bounamous et al, 2008Bounamous et al, , 2014Lins et al, 2008…”

Section: Resultsmentioning

confidence: 99%

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

Depaquit

2014

Infection, Genetics and Evolution

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Genetic divergence between two sympatric species of the Lutzomyia longipalpis complex in the paralytic gene, a locus associated with insecticide resistance and lovesong production

Cited by 35 publications

References 27 publications

Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males

Genetic differentiation in natural populations of Lutzomyia longipalpis (Lutz & Neiva) (Diptera: Psychodidae) with different phenotypic spot patterns on tergites in males

Knockdown resistance mutations predict DDT resistance and pyrethroid tolerance in the visceral leishmaniasis vector Phlebotomus argentipes

Molecular systematics applied to Phlebotomine sandflies: Review and perspectives

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