Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil

Miranda, Karla Rodrigues; Dias, Mariana F; Guimarães, Priscilla L.S.; Boente, Renata F.; Pauer, Heidi; Ramos, Priscila Zonzini; Falcão, Laís S.; Ferreira, Eliane de Oliveira; Balassiano, Ilana Teruszkin; Ferreira, Livia Q.; Santos-Filho, Joaquim dos; Paula, Geraldo Renato de; Antunes, Eduardo N.F; Avelar, Kátia Eliane Santos; Domingues, Regina Maria Cavalcanti Pilotto

doi:10.1590/s0074-02762008000700018

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…Nineteen cases (12.1%) including bft gene were discovered in diarrheal feces (14 cases) and non-diarrheal feces (5 cases). These results are in accordance with many obtained results in the world (15), however are in contrast with the results reported in Brazil which are significantly lower than this number (19).…”

Section: Discussionsupporting

confidence: 91%

“…Recently ETBF isolation from healthy children and adults' feces and also from those with diarrhea was reported (14). Researchers have discov-ered B. fragilis enterotoxin directly from a part of feces sample whose culture hadpositive results for ETBF (14) and different studies in Brazil reported 1.5-3% ETBF among stool samples (19). In other studies high numbers of ETBF have been reported in different points of the world (3,20), for instance Kato et al(1996) reported a percentage of 18.5% in Japan, while it was about 14% in Poland and Holland (21).…”

Section: Discussionmentioning

confidence: 99%

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Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools

Akhi

Ghotaslou

Shirinzadeh

et al. 2013

Jundishapur J Microbiol

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Background: Enterotoxigenic Bacteroides fragilis (ETBF) are one of the most important anaerobic bacteria whichcause diarrhea in human beings. A bft gene is coded for enterotoxin production called fragilysin. Most of them have acquired resistance to antianaerobicbacteria agent like other facultative anaerobic bacteria. E test and different dilution methods are usually performed for antimicrobial susceptibility determination of B. fragilis.Objectives: The aims of this study are to recognize ETBF by PCR method and also to evaluate efficiency thedisk diffusion method (DDM) in comparison with the E tests for antimicrobial susceptibility of B. fragilis isolates.Materials and Methods: bft gene was detected among 157 B. fragilis isolated from patients and healthy individuals by PCR. Antimicrobial susceptibility of all isolates was determined by DDM and E tests methods.Results: Nineteen (12.1%) B. fragilis containing bft gene from diarrheic (n = 14) and nondiarrheic (n = 5) feces were detected among 157 B. fragilis isolates. The highest resistance for ciprofloxacin, cefotaxim, cefoxitin with DDM and E test were 100%, 60%, and 65% respectively, while the lowest resistance in two methods was obtained for imipenem, piperacillin / tazobactam, and metronidazol. 100% agreement for some of antibiotics such as imipenem (P ≤ 0.05), and no correlation for others were observed among the antimicrobial susceptibility results obtained by the two methods (P ≥ 0.05).Conclusions: The presence of bft gene in B. fragilis isolates wouldnot certainly result in diarrheaamong patients. There is not enough accordance between DDM and Swedish E tests for antimicrobial susceptibility of B. fragilis for some antibiotics, although in other cases a good agreement was observed

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools

Akhi

Ghotaslou

Shirinzadeh

et al. 2013

Jundishapur J Microbiol

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“…In a relevant work using nontoxigenic B. fragilis ATCC 23745 strain (Miranda et al, 2008) similar to that of our study, the phenylacetic acid (PA) of the sonicated extract could both induced vacuolating effect on Vero cells and peritoneal macrophages and increased apoptotic cell death (Falcão et al, 2015).…”

Section: Discussionsupporting

confidence: 81%

Untitled

2023

JJBS

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Study areas include cell biology, genomics, microbiology, immunology, molecular biology, biochemistry, embryology, immunogenetics, cell and tissue culture, molecular ecology, genetic engineering and biological engineering, bioremediation and biodegradation, bioinformatics, biotechnology regulations, gene therapy, organismal biology, microbial and environmental biotechnology, marine sciences. The JJBS welcomes the submission of manuscript that meets the general criteria of significance and academic excellence. All articles published in JJBS are peerreviewed. Papers will be published approximately one to two months after acceptance.

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Section: Discussionsupporting

confidence: 81%

Bacteroides fragilis Induce Apoptosis and subG1/G1 Arrest Via Caspase and Nrf2 Signaling Pathways in HT-29 Cell Line

2023

JJBS

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Background: Although the link between the human microbiome and colorectal cancer has been elucidated over recent years, its underlying mechanisms for various bacteria such as nontoxigenic Bacteroides fragilis (NTBF) are still unclear. Methods: HT29 cells were treated with Non-toxigenic B. fragilis Sonicated Extract (NBF-SE), strain ATCC-23745, in the dose (10 8 and 10 9 CFU/ml) -and time (24 and 72 hours)-dependent manners. To investigate the cytotoxic impact of NBF-SE on HT29 along with apoptosis-induction and cell cycle distribution, MTT assay and flow cytometry was carried out. Changes in the expression level of Keap1/NRF2/Caspase3/caspase10/GCLM proposed signaling pathway were conducted by real time PCR analyses. Results: We found that the NBF-SE had an antiproliferative effect on HT29 cells. In addition, NBF-SE induced sub-G1 cell cycle cessation and an increment in cell apoptosis, all in a dose-and time-dependent manner. Further, NBF-SE significantly up-regulated Caspase10 and Caspase3 genes, correlated to increased apoptosis. However, Kelch-like ECH-associated protein 1 (KEAP1) and Nuclear factor erythroid 2-related factor 2 (Nrf2) as well as Glutamate-cysteine ligase modulator (GCLM)mediated antioxidation and anti-apoptosis pathway genes were upregulated, which was in contradiction with apoptosis activation. Conclusions: We revealed NTBF as a new inducer of cell death confirmed by cytotoxic activity, cell cycle arrest and, increased apoptosis together with NTBF's greater potency in the activation of caspase apoptotic signaling pathway over NRF2/ GCLM antioxidant response. Moreover, NTBF strain ATCC-23745 may be a hidden probiotic and be used for further studies in CRC treatment.

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Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil

Cited by 6 publications

References 15 publications

Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools

Comparison of E test and Disk Diffusion Test for Antibiotic Resistance Testing of Enterotoxigenic and Non- Enterotoxigenic Bacteroides fragilis Isolated From Stools

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Bacteroides fragilis Induce Apoptosis and subG1/G1 Arrest Via Caspase and Nrf2 Signaling Pathways in HT-29 Cell Line

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