Trypanosoma cruzi: blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs

Veloso, Vanja Maria; Guedes, Paulo M. M.; Andrade, Isabel Mayer de; Caldas, Ivo Santana; Martins, Helen Rodrigues; Carneiro, Cláudia Martins; Machado-Coelho, George Luiz Lins; Lana, Marta de; Galvao, L. M. C.; Bahia, Maria T.; Chiari, Égler

doi:10.1590/s0074-02762008000600003

Cited by 21 publications

(14 citation statements)

References 29 publications

(29 reference statements)

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“…This confirms the results obtained by Veloso and others 43 in blood samples, which found increased sensitivity of this technique in the acute phase. The number of blood samples positive for PCR decreased during the chronic phase, and similar results were observed in the chronic model of T. cruzi infection in guinea pigs.…”

Section: Discussionsupporting

confidence: 92%

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Yauri

Castro-Sesquen

Verástegui

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. Pigs were infected with a Bolivian strain of Trypanosoma cruzi (genotype I) and evaluated up to 150 days postinoculation (dpi) to determine the use of pigs as an animal model of Chagas disease. Parasitemia was observed in the infected pigs during the acute phase (15-40 dpi). Anti-T.cruzi immunoglobulin M was detected during 15-75 dpi; high levels of anti-T.cruzi immunoglobulin G were detected in all infected pigs from 75 to 150 dpi. Parasitic DNA was observed by western blot (58%, 28/48) and polymerase chain reaction (27%, 13/48) in urine samples, and in the brain (75%, 3/4), spleen (50%, 2/4), and duodenum (25%, 1/4), but no parasitic DNA was found in the heart, colon, and kidney. Parasites were not observed microscopically in tissues samples, but mild inflammation, vasculitis, and congestion was observed in heart, brain, kidney, and spleen. This pig model was useful for the standardization of the urine test because of the higher volume that can be obtained as compared with other small animal models. However, further experiments are required to observe pathological changes characteristic of Chagas disease in humans.

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Section: Discussionsupporting

confidence: 92%

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Yauri

Castro-Sesquen

Verástegui

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…Furthermore, the denervation process and GFAP + glial cell reduction caused by this strain were significant only in the acute phase, returning to homeostasis at two years post-infection. These data are consistent with faster parasitaemia ( Veloso et al 2008 ) and control of tissue parasitism. In contrast, the persistence of parasites in the Be-78 group related to the absence of clinical symptoms in chronic experimental ChD suggests the participation of other factors, such as host genetics and immune response and phenomena associated with the parasite-host interaction, during the acute phase development of the ChD indeterminate form.…”

Section: Discussionsupporting

confidence: 85%

“…These distinct profiles indicate infection with polyclonal strains and a preferential histotropism between the populations, sufficient to influence the distinct clinical forms’ pathogenesis and development ( Vago et al 1996 ). Although in this work infection with two strains was independent, a distinct selection may be suggested in the chronic phase, since heart tissues show a higher positivity by PCR for the Y strain ( Veloso et al 2008 ), while the oesophagus was positive only for Be-78 strain in our study. This hypothesis is further strengthened by data obtained by Guedes et al (2007) during an evaluation of Beagle hearts, in which 80% of animals Y-infected showed cardiomegaly and cardiac abnormalities, chronic inflammation and fibrosis.…”

Section: Discussionmentioning

confidence: 48%

Myenteric plexus is differentially affected by infection with distinct Trypanosoma cruzi strains in Beagle dogs

Paiva

Fonseca

Vieira

et al. 2014

Mem. Inst. Oswaldo Cruz

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Chagasic megaoesophagus and megacolon are characterised by motor abnormalities related to enteric nervous system lesions and their development seems to be related to geographic distribution of distinct Trypanosoma cruzi subpopulations. Beagle dogs were infected with Y or Berenice-78 (Be-78) T. cruzi strains and necropsied during the acute or chronic phase of experimental disease for post mortem histopathological evaluation of the oesophagus and colon. Both strains infected the oesophagus and colon and caused an inflammatory response during the acute phase. In the chronic phase, inflammatory process was observed exclusively in the Be-78 infected animals, possibly due to a parasitism persistent only in this group. Myenteric denervation occurred during the acute phase of infection for both strains, but persisted chronically only in Be-78 infected animals. Glial cell involvement occurred earlier in animals infected with the Y strain, while animals infected with the Be-78 strain showed reduced glial fibrillary acidic protein immunoreactive area of enteric glial cells in the chronic phase. These results suggest that although both strains cause lesions in the digestive tract, the Y strain is associated with early control of the lesion, while the Be-78 strain results in progressive gut lesions in this model.

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“…The values observed with this technique, in experimental studies with dogs, reached more than 90% using blood or others tissues (ARAÚJO et al, 2002;VELOSO et al, 2008). Veloso et al (2008) obtained the highest levels in identifying parasitized experimentally infected Beagle dogs that were in the acute phase of infection, when the parasitemia levels were high. The low incidence observed in this study can be explained by the fact that those animals were possibly on the chronic phase of the infection or were not really infected.…”

Section: Resultsmentioning

confidence: 88%

First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

Júnior

Almeida

Carvalho

et al. 2013

Rev. Bras. Parasitol. Vet.

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In order to verify the Trypanosoma cruzi infection in domestic domiciled dogs in a rural endemic area from the south region of the State of Bahia, Polymerase Chain Reaction (PCR) were performed using S35 and S36 primers in 272 dogs living in the district of Vila Operaria, in the municipality of Buerarema. All animals were clinically evaluated; 2.5 mL of blood were collected through venipuncture for the performance of molecular tests. None of these animals showed clinical signs of the illness and only two were identified with the DNA parasite. This result is the first report of natural infection by T. cruzi in domestic dogs in southern Bahia.Keywords: Trypanosoma cruzi, dog, PCR, Polymerase Chain Reaction, Bahia. ResumoCom o objetivo de verificar a infecção por Trypanosoma cruzi em cães domésticos domiciliados em área rural e endêmica do sul da Bahia, foi realizada a Reação em Cadeia da Polimerase (PCR), utilizando-se os iniciadores S35 e S36 em 272 cães domiciliados no distrito da Vila Operária, cidade de Buerarema. Todos os animais foram avaliados clinicamente e, posteriormente, foram coletados 2,5 mL de sangue por punção venosa para realização do diagnóstico molecular. Nenhum dos animais apresentou manifestação clínica da doença e, em apenas dois foram identificados DNA do parasito. Esse resultado é o primeiro relato de infecção natural por T. cruzi em cães domésticos no sul baiano.

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Trypanosoma cruzi: blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs

Abstract: The goals of the present study were to evaluate the kinetics of blood parasitism by examination of fresh blood, blood culture (BC)

Cited by 21 publications

References 29 publications

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection

Myenteric plexus is differentially affected by infection with distinct Trypanosoma cruzi strains in Beagle dogs

First report of Trypanosoma cruziinfection in naturally infected dogs from southern Bahia, Brazil

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