Genetic relationships between sympatric populations of Bacillus cereus and Bacillus thuringiensis, as revealed by rep-PCR genomic fingerprinting

Peruca, Ana Paula S.; Vilas-Bôas, Gislayne Trindade; Arantes, O. M. N.

doi:10.1590/s0074-02762008000500016

Cited by 11 publications

(6 citation statements)

References 15 publications

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“…Several alternative typing methods for B. thuringiensis strains, mostly based on molecular techniques, have been recently described [Cherif et al, 2007;Gaviria Rivera and Priest, 2003a, b;Joung and Côte, 2001;La Duc et al, 2004;Lima et al, 2002;Manzano et al, 2009;Peruca et al, 2008;Ibarra, 2005, 2008;Xu and Côte, 2006;Yu et al, 2002]. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep) sequence-based PCR fingerprinting technique was shown to be highly discriminative, fast, easy, and able to identify B. thuringiensis serotypes, including non-flagellar and self-agglutinated strains [Reyes-Ramirez and Ibarra, 2005].…”

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confidence: 99%

Evidence of Bacillus thuringiensis Intra-Serovar Diversity Revealed by Bacillus cereus Group-Specific Repetitive Extragenic Palindromic Sequence-Based PCR Genomic Fingerprinting

Sauka¹,

Basile²,

Benintende³

2011

Microb Physiol

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Bacillus thuringiensis is classified into serovars on the basis of H-flagellar antigens. Several alternative typing methods have been described. Among them, a B. cereus group-specific repetitive extragenic palindromic (Rep)-PCR fingerprinting technique was shown to be discriminative and able to identify B. thuringiensis serovars. The aim of this study was to investigate the genomic diversity and relationship among B. thuringiensis strains collected from different Argentinean ecosystems. Thirty-seven B. thuringiensis reference strains and 131 Argentinean isolates were analyzed using a B. cereus group-specific Rep-PCR. Fourteen different patterns were identified among the Argentinean isolates. Eight could not be associated to any pattern obtained from a reference strain. The pattern identical to the serovar kurstaki HD-1 strain was the most frequently identified in 68 native isolates. The profiles allowed tracing a single dendrogram with two groups and eight main lineages. Some strains showed distinctive patterns despite belonging to the same serovar. An intraspecific diversity resulted from this analysis that was highlighted by this technique since strains from a given serovar showed distinct profiles. This study may help to establish a system of B. thuringiensis classification with a higher discrimination level than established by the H antigen serotyping.

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confidence: 99%

Evidence of Bacillus thuringiensis Intra-Serovar Diversity Revealed by Bacillus cereus Group-Specific Repetitive Extragenic Palindromic Sequence-Based PCR Genomic Fingerprinting

Sauka¹,

Basile²,

Benintende³

2011

Microb Physiol

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“…After exhaustive studies using DNA-DNA hybridization, 16S and 23S rRNA comparative analyses, multilocus sequence typing (MLST), fluorescent amplified fragment length polymorphism analysis, rep-PCR, and small nucleotide polymorphism (SNP) analyses, scientists have been unable to reliably differentiate these three Bacillus species. While many methods have been pursued, most results have suggested that B. cereus, B. thuringiensis, and B. anthracis should be considered the same species due to highly conserved nucleoidal genetic sequences (3,17,39,43,48). Due to the easily identifiable symptoms of B. anthracis and B. cereus, there is recent concern among biologists that the "B. anthracis" species may in fact be an oversampled subset of B. cereus (42).…”

Section: Discussionmentioning

confidence: 99%

“…These species are also grouped under the name B. cereus sensu lato (51). Phylogenetically, B. cereus is quite closely related to the entomopathogen B. thuringiensis and the human pathogen B. anthracis, a fact that has led to vigorous discussion on shared virulence properties, DNA sequence conservation among strains, and prevalence in the environment (19,39,48). Along with B. weihenstephanensis, these species constitute a single genetic subgroup, a rather arbitrary classification designation that brings into question how a species is even defined in this family of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Real-time Screening of Foods Using Repetitive Element PCR Reveals a DNA Marker Characteristic for Enterotoxigenic Bacillus Species

et al. 2021

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Bacillus cereus is traditionally thought to be the only member of its genus accepted as a pathogen in foods like grains, fruits, vegetables, and milk due to the presence of the nonhemolytic (Nhe) operon. However, many other Bacillus spp. may also harbor the Nhe operon and be pathogenic, including not just food-associated gastrointestinal toxicoinfections, but human endophthalmitis as well. Real-time PCR targeted the nheA gene in 37 samples obtained from food, soil, and reference cultures by analyzing the standard deviations of melt peaks. Repetitive element PCR was used to compare the banding patterns of each sample against B. cereus ATCC 14579 and three B. thuringiensis strains to “fingerprint” each isolate. Of the original 43 isolated tested, 37 were Gram-positive rods. The remaining six samples were Gram-positive cocci. Twenty-five of the 37 Gram-positive Bacillus spp. were nheA positive, while twelve were negative. Many of the nheA positive strains were species not previously known to contain Nhe and were capable of causing gastroenteritis in consumers.

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“…However, there are no sufficiently convincing results, and this makes it a bit difficult to classify both species. According to Peruca et al [50] sometimes both species are classified together, whereas at other times B. thuringiensis is categorized as a subspecies of B. cereus which really makes the classification so complicated.…”

Section: Identification and Characterization Of Ferulic Acid-producinmentioning

confidence: 99%

Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation using banana stem waste

et al. 2020

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Exploitation of soil bacteria for production of ferulic acid (FA) is extensively performed since bacteria are the largest soil community that have the potential in producing degrading enzymes. This study aims to isolate, identify and characterize the most efficient soil bacteria for high FA yield via co-culture fermentation of banana stem waste (BSW). Bacteria were isolated and screened from acclimatized mixture of soil culture and BSW. ARB programme package and biolog system were employed for identification and characterization. The results reveal that four isolates closely related to Bacillus spp. and one Lysinibacillus sp. had greater potential to produce FA in very large amounts. Specifically, the maximum FA yield of 394.76 mg/kg was achieved using co-culture of Bacillus sp. MB2, Bacillus sp. WB8A and B. pumilus strain WB1A, which was 2.5-fold higher than FA produced by single culture. The profiles of substrate utilization exposed strong hydrolyzation of pectin in those three potential cultures, while one showed strong hydrolyzation of glucuronic acid. The ability to efficiently hydrolyze the components proves that the chosen co-cultures are good sources of hydrolytic enzymes. The results suggest that the co-culture has contributed cooperative actions among the cultures to synergistically breakdown the FA linkage in BSW to produce high FA yield.

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Genetic relationships between sympatric populations of Bacillus cereus and Bacillus thuringiensis, as revealed by rep-PCR genomic fingerprinting

Cited by 11 publications

References 15 publications

Evidence of Bacillus thuringiensis Intra-Serovar Diversity Revealed by Bacillus cereus Group-Specific Repetitive Extragenic Palindromic Sequence-Based PCR Genomic Fingerprinting

Evidence of Bacillus thuringiensis Intra-Serovar Diversity Revealed by Bacillus cereus Group-Specific Repetitive Extragenic Palindromic Sequence-Based PCR Genomic Fingerprinting

Real-time Screening of Foods Using Repetitive Element PCR Reveals a DNA Marker Characteristic for Enterotoxigenic Bacillus Species

Isolation, identification and characterization of soil bacteria for the production of ferulic acid through co-culture fermentation using banana stem waste

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