Ultrastructural description of rabies virus infection in cultured sensory neurons

Mapping Sensory Circuits by Anterograde Transsynaptic Transfer of Recombinant Rabies Virus

“…Rigorous exclusion of the possibility that spinal interneurons classified as pre-motor have been labeled by anterograde transfer from a sensory neuronal source requires information on the occurrence and selectivity of RV infection and RV-G expression by sensory neurons. Our studies, and those of others (Velandia et al, 2007; Velandia-Romero et al, 2013), document the propensity of sensory neurons, including proprioceptors, for infection by RV. In turn these findings raise the issue of the degree of sensory tropism achieved with different experimental protocols.…”

Intergenotypic Replacement of Lyssavirus Matrix Proteins Demonstrates the Role of Lyssavirus M Proteins in Intracellular Virus Accumulation

“…One example is the neurotropic Oita rhabdovirus that combines several characteristics of lyssa-and vesiculoviruses and was shown to produce enveloped viruslike structures in the ER (23). Although no experimental data corroborate previous speculations about the role of the intracellular assembly structures in intraneuronal RNP transport (43), this hypothesis is attractive in terms of intraneuronal long-distance transport of newly synthesized RNPs or viruslike structures without the need of budding to the extracellular space. This would also explain why in standard cell cultures the intracellular RNP accumulations do not contribute to the production of infectious virus.…”

“…4A and B), indicating that budding events have occurred at the rER membrane. Accumulation of intracellular viruslike structures is a common phenomenon in RABV-infected cell cultures and even in primary neurons or neuronal tissues (22,24,28,29,43). The lack of comparable structures in SAD EBLV-1 M-and SAD EBLV-2 M-infected cells, therefore, was surprising, in particular since budding of typical bullet shaped virus particles at the plasma membrane was observed (Fig.…”

The Fatal Case of Lyssavirus Encephalitis in the Russian Far East

“…The oval and dense bodies (with diameter about 2550 nm) were determinated in cytoplasm and mainly in extracellular environment of PEG cells culture infected by strain Ozernoe. These structures were described earlier as Negri bodies in neurons infected by Rabies virus [Velandia et al, 2007].…”