Paleogenetic and taphonomic analysis of human bones from Moa, Beirada, and Zé Espinho Sambaquis, Rio de Janeiro, Brazil

Marinho, Andrey Moacir do Rosário; Miranda, Newton Cardoso; Braz, Valéria; Ribeiro-dos-Santos, Ândrea; Souza, Sheila Maria Ferraz Mendonça de

doi:10.1590/s0074-02762006001000004

Cited by 12 publications

(13 citation statements)

References 33 publications

(38 reference statements)

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“…The extraction and treatment of the DNA samples were performed in the Paleogenetic section of the Human and Medical Genetics Laboratory at the Federal University of Pará (UFPA) according to the established protocol [25,32]. The DNA was extracted either with the phenol-chloroform method [33] with modifications or with the DNA IQ System kit (Promega, Madison, WI, USA).…”

Section: Extraction Pcr and Sequencingmentioning

confidence: 99%

Paleogenetic Studies in Guajajara Skeletal Remains, Maranhão State, Brazil

Leite

Leitão

Schaan

et al. 2014

Journal of Anthropology

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In the early 17th century, French and Portuguese colonizers and Jesuit priests settled in the state of Maranhão and made contact with the Guajajara, an ethnic group that lived along the margins of the Pindaré River. The Guajajara maintained contact with Brazilian national society over the centuries, including with Brazilian admixed populations, and with African slaves that flocked towards the region from the 18th century onwards. The present study investigates the origins of this admixture using mitochondrial genetic variability. The bones of 12 individuals investigated, which are currently part of the collection of the National Museum, were tested for genetic diversity. aDNA was extracted by the phenol-chloroform method and by DNA IQ (Promega, Madison, WI, USA). Amplification of the HVS I region was performed by PCR, followed by direct sequencing using the Big Dye kit (Life Technologies, Foster City, CA, USA). This region was found to represent haplogroups of Amerindians (A, C, and D) and Africans (L, L1b, L1c, and L3). The presence of African haplogroups in Guajajara bones from as early as the 18th century is consistent with historical and anthropological data, suggesting the admixture with Africans and/or Afrodescendants. Therefore, this study demonstrates that women with African haplogroups were introduced into the Guajajara population.

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Section: Extraction Pcr and Sequencingmentioning

confidence: 99%

Paleogenetic Studies in Guajajara Skeletal Remains, Maranhão State, Brazil

Leite

Leitão

Schaan

et al. 2014

Journal of Anthropology

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“…Furthermore, the preservation of DNA in archaeological bones has been correlated with various factors including nearly perfect micromorphology, with only small areas of localized demineralization [30], lamellae integrity [7], a more compact appearance of bone in scanning electron micrographs and high collagen content [8]. We therefore analyzed the medieval human bone samples by transmission electron microscopy, which is a powerful tool that has been used to study the ultrastructural characteristics of both archaeological and fossilized bones [31,32].…”

Section: Discussionmentioning

confidence: 99%

“…It is also important to know how well the bones have been preserved at a microscopic level since this has been shown to reflect the integrity of the isolated DNA [7,8]. This…”

Section: Page 4 Of 41mentioning

confidence: 99%

“…However, analysis at a microscopic level can provide an indication as to the preservation of amplifiable DNA; the isolation of amplifiable DNA has been correlated with integrity of the microscopic structure, particularly lamellae [7]. In addition, more compact appearance of bone in scanning electron micrographs is one of the factors that indicates that amplifiable DNA can be isolated [8].…”

Section: Introductionmentioning

confidence: 99%

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DNA and bone structure preservation in medieval human skeletons

Coulson-Thomas

Norton

Coulson‐Thomas

et al. 2015

Forensic Science International

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Nader, DNA and bone structure preservation in medieval human skeletons, Forensic Science International (2015), http://dx.doi.org/10.1016/j.forsciint. 2015.04.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t AbstractMorphological and ultrastructural data from archaeological human bones are scarce, particularly data that have been correlated with information on the preservation of molecules such as DNA. Here we examine the bone structure of macroscopically wellpreserved medieval human skeletons by transmission electron microscopy and immunohistochemistry, and the quantity and quality of DNA extracted from these skeletons. DNA technology has been increasingly used for analyzing physical evidence in archaeological forensics; however, the isolation of ancient DNA is difficult since it is highly degraded, extraction yields are low and the co-extraction of PCR inhibitors is a problem. We adapted and optimized a method that is frequently used for isolating DNA from modern samples, Chelex® 100 (Bio-Rad) extraction, for isolating DNA from archaeological human bones and teeth. The isolated DNA was analysed by real-time PCR using primers targeting the sex determining region on the Y chromosome (SRY) and STR typing using the AmpFlSTR® Identifiler PCR Amplification kit. Our results clearly show the preservation of bone matrix in medieval bones and the presence of intact osteocytes with well preserved encapsulated nuclei. In addition, we show how effective Chelex® 100 is for isolating ancient DNA from archaeological bones and teeth. This optimized method is suitable for STR typing using kits aimed specifically at degraded and difficult DNA templates since amplicons of up to 250 bp were successfully amplified.

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“…Localizado em Saquarema, entre o mar e a lagoa, este sítio apresenta três datações de 3610 ± 190, 3810+50 e 3960 ± 200 e AP (Kneip & Machado, 1993;Marinho et al, 2006).…”

Section: Moaunclassified

Diversidade morfológica craniana, micro-evolução e ocupação pré-histórica da costa brasileira

Okumura

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Paleogenetic and taphonomic analysis of human bones from Moa, Beirada, and Zé Espinho Sambaquis, Rio de Janeiro, Brazil

Abstract: The present paper discusses mtDNA and taphonomy of human remains from Moa, Beirada, and

Cited by 12 publications

References 33 publications

Paleogenetic Studies in Guajajara Skeletal Remains, Maranhão State, Brazil

Paleogenetic Studies in Guajajara Skeletal Remains, Maranhão State, Brazil

DNA and bone structure preservation in medieval human skeletons

Diversidade morfológica craniana, micro-evolução e ocupação pré-histórica da costa brasileira

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