Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni

Sarvel, Ana Karine; Kusel, J. R.; Araújo, Neusa; Coelho, Paulo Marcos Zech; Katz, Naftale

doi:10.1590/s0074-02762006000900045

Cited by 16 publications

(28 citation statements)

References 5 publications

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“…These results indicated that PZQ is lethal to immature and mature eggs as it caused impairment of egg production which may be due to degenerative changes of the reproductive organs of female worms confirmed by many investigators. (Sarvel AK et al 2006, Conceiço MJ et al 2008, Holtfreter MC et al 2011) NTZ resulted in significant reduction in immature eggs, but not more than 18.5% compared to non-treated control. It could rise the percentage of dead eggs to 30.5% and mature eggs to 51% at different intervals of follow up.…”

Section: Discussionmentioning

confidence: 80%

Comparative parasitological and electron microscopic studies on the effects of Nitazoxanide and Praziquantel in Schistosoma mansoni-infected mice

El-Taweel¹,

El-Sayad²,

Helw³

et al. 2016

IJPT

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This study was designed to evaluate antischistosomal activity of Nitazoxanide (NTZ) in Schistosoma mansoni-infected mice compared to Praziquantel (PZQ). Fifty four infected mice were recruited into 3 groups, each of 18 mice. Group 1 was infected non-treated control. Group 2 was infected and then treated with PZQ 500 mg for two days, and group 3 was infected and treated with NTZ 100 mg/kg for seven days. Efficacy of drugs was assessed by Parasitological, and scanning electron microscopic studies. PZQ reduced (4.9%, 22.5% and 50.7%) of faecal eggs, (22%, 22.6% and 55.1%) of intestinal eggs, (20.4%, 44.3% and 46.7%) of hepatic egg counts and (27%, 45.1% and 64.9%) of total worm load whereas, NTZ reduced (4.9%, 22.5% and 50.7%),of faecal eggs, (22%, 22.6% and 55.1%) of intestinal eggs ,(20.4%, 44.3% and 46.7%) of hepatic egg counts and (27%, 45.1% and 64.9%) of total worm load at 1, 2 and 4 WPT, respectively. The percentages of dead eggs were more than 80% after PZQ treatment and only 30% after NTZ at 4 WPT. PZQ showed extensive tegumental damages in male and female worms more than NTZ at 2 WPT. Our findings concluded that Nitazoxanide showed weaker antischistosomal activity in animal models than praziquantel.

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Section: Discussionmentioning

confidence: 80%

Comparative parasitological and electron microscopic studies on the effects of Nitazoxanide and Praziquantel in Schistosoma mansoni-infected mice

El-Taweel¹,

El-Sayad²,

Helw³

et al. 2016

IJPT

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“…After an 8-day-period of observation, the total number of morphologically dead eggs in the groups exposed to 2 µg/ml and 4 µg/ml lovastatin in vitro was 44 (73.3%) and 348 (94.8%), respectively; in the control group it was 144 (27.5%). Using the probe Hoechst 33258, a fluorescent marker that label dead eggs (Sarvel et al 2006) it was observed that in the group exposed to 2 µg/ml lovastatin, 69% of the morphologically viable eggs were labeled by the probe, whereas in the group exposed to 4 µg/ml lovastatin, 74% of the eggs were labeled. In the control group, 16% of the morphologically viable eggs were labeled by the probe (p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…Since egg labeling by the probe indicates that the egg is dead, the difference in the classification of viable eggs is evident when the probe is used in comparison when only the morphology of the egg is observed under an optic microscope. When applied in assays without drug utilization, the methodology recommended by Sarvel et al (2006) with the aim to confirm the traditional morphological classification, 83% of corroboration between the two methods was detected. Nevertheless, in the trials related to drug activity was much lower (30%), i.e., only 10 out of 35 morphologically viable eggs present in the groups exposed to 2 µg/ml or 4 µg/ml lovastatin were not labeled by the probe.…”

Section: Discussionmentioning

confidence: 96%

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Oxamniquine, praziquantel and lovastatin association in the experimental Schistosomiasis mansoni

Araújo

Mattos

Sarvel

et al. 2008

Mem. Inst. Oswaldo Cruz

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In practice, chemotherapy is the main measure utilized for the control of schistosomiasis in endemic countries. The schistosomicidal drugs used, oxamniquine and praziquantel, present few side effects and a high schistosomicidal activity, thus contributing for the treatment of infection, as well as for the control of morbidity and transmission of the disease. In the 1970s, oxamniquine was very used for individual and mass treatment of schistosomiasis, presenting satisfactory results regarding efficacy and tolerance (Katz 1980). At the end of that decade, praziquantel became available for the treatment of schistosomiasis, showing good therapeutic activity and also few side effects as well. Praziquantel is now the drug of choice for the treatment of the disease caused by the three main species of the parasite that infect humans (Doenhoff et al. 2002). Taking into account the possible rise of resistance to treatment, the tolerance level of the worm to the drug, and the therapeutic failures, it is very important to search for new therapeutic alternatives for schistosomiasis. This search is now imperative, especially considering the possible interruption of oxamniquine production as a result of its higher cost, when compared to praziquantel.The association of different drugs for use in the therapeutics of various infectious diseases can serve as a mechanism to avoid, or to delay, the rise of drug resistance. In the present study, in vivo and in vitro trials were carried out on the efficacy of the treatment with lovastatin against the Schistosoma mansoni adult worms, when administered in association with oxamniquine or praziquantel in the mouse model.

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“…In contrast, non-fluorescent dyes such as methylene blue (MB), trypan blue (TB), and neutral red (NR) have often been used to assess the viability of schistosome schistosomula, eggs and miracidia (10,11). Nevertheless, to the best of our A c c e p t e d M a n u s c r i p t antischistosomal effect of screening compounds on schistosome adult worms.…”

Section: Introductionmentioning

confidence: 99%

Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on <i>Schistosoma mansoni </i>Adult Worms

Mitsui

Kato

2018

Jpn J Infect Dis

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A c c e p t e d M a n u s c r i p tKey words: methylene blue, neutral red, trypan blue, antimalarial drug, antischistosomal effect A c c e p t e d M a n u s c r i p t Furthermore, the severity of the damage to the adult worms treated with the three drugs might be reflected in the TB staining status. The present study shows that these non-fluorescent dyes can serve as a useful complementary tool for assessing the antischistosomal effect of antimalarial drugs.

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Comparison between morphological and staining characteristics of live and dead eggs of Schistosoma mansoni

Cited by 16 publications

References 5 publications

Comparative parasitological and electron microscopic studies on the effects of Nitazoxanide and Praziquantel in Schistosoma mansoni-infected mice

Comparative parasitological and electron microscopic studies on the effects of Nitazoxanide and Praziquantel in Schistosoma mansoni-infected mice

Oxamniquine, praziquantel and lovastatin association in the experimental Schistosomiasis mansoni

Application of Non-Fluorescent Dyes to Assess the Antischistosomal Effect of Antimalarial Drugs on <i>Schistosoma mansoni </i>Adult Worms

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