Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil

Araújo, Fernanda Montenegro de Carvalho; Nogueira, Rita Maria Ribeiro; Araújo, Josélio Maria Galvão de; Ramalho, Izabel Letícia Cavalcante; Roriz, Maria Lucia Feitosa de Sá; Melo, Maria Elizabeth Lisboa de; Coêlho, Ivo Castelo Branco

doi:10.1590/s0074-02762006000800017

Cited by 37 publications

(24 citation statements)

References 19 publications

(21 reference statements)

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“…Other simultaneous Flavivirus infections, especially those caused by different dengue virus types, such as DENV-1 and 2 22,36 , DENV-2 and 3 3,22,43,47,48 , DENV-1 and 3 26 , DENV-3 and DENV-4 14,15 have been reported. Hence, co-infection with distinct DENV serotypes during outbreaks may be expected.…”

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confidence: 99%

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Colombo

Vedovello

Reis

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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confidence: 99%

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Colombo

Vedovello

Reis

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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“…Although DENV hyperendemicity (with the simultaneous circulation of different CONCURRENT DENGUE AND VIVAX MALARIA viruses) is quite common in tropical areas, co-infection with multiple viruses has been scarcely reported with a few case reports from Taiwan, Puerto Rico, and Brazil. [21][22][23] In 2011, this phenomenon was reported during an outbreak in our region, and was not related to severity. 24 Even without the proper design to estimate if co-infection is associated with more severe complications, this case series describes a high frequency of persistent vomiting, abdominal pain, and bleeding, which are well-known warning signs for severe dengue.…”

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confidence: 99%

Clinical Profile of Concurrent Dengue Fever and Plasmodium vivax Malaria in the Brazilian Amazon: Case Series of 11 Hospitalized Patients

Magalhães¹,

Alexandre²,

Siqueira³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Malaria and dengue fever are the most prevalent vector-borne diseases worldwide. This study aims to describe the clinical profile of patients with molecular diagnosis of concurrent malaria and dengue fever in a tropicalendemic area. Eleven patients with concurrent dengue virus (DENV) and Plasmodium vivax infection are reported. Similar frequencies of DENV-2, DENV-3, and DENV-4 were found, including DENV-3/DENV-4 co-infection. In eight patients, the World Health Organization (WHO) criteria for severe malaria could be fulfilled (jaundice being the most common). Only one patient met severe dengue criteria, but warning signs were present in 10. Syndromic surveillance systems must be ready to identify this condition to avoid misinterpretation of severity attributed to a single disease.

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“…The presence of DENV has been previously detected in A. aegypti and other mosquito species 8 , and DENV co-infections have been reported in clinical samples 9 . These findings of the present study in A. aegypti mosquitoes corroborate the detection of DENV in clinical samples and reinforces the need for strengthened epidemiological surveillance of dengue infection by the Health authorities.…”

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confidence: 89%

Identification of dengue viruses in naturally infected Aedes aegypti females captured with BioGents (BG)-Sentinel traps in Manaus, Amazonas, Brazil

Figueiredo

Mourão

Abi-Abib

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:In Manaus, the first autochthonous cases of dengue fever were registered in 1998. Since then, dengue cases were diagnosed by the isolation of viruses 1, 2, 3, and 4. Methods: One hundred eighty-seven mosquitoes were collected with BioGents (BG)-Sentinel traps in 15 urban residential areas in the Northern Zone of Manaus and processed by molecular tests. Results: Infections with dengue viruses 1, 2, 3, and 4 and a case of co-infection with dengue viruses 2 and 3 were identified. Conclusions: These findings corroborate the detection of dengue in clinical samples and reinforce the need for epidemiological surveillance by the Health authorities.Keywords: Aedes aegypti. Dengue. Virus. Molecular tests.Manaus, Amazonas (AM) was infested by Aedes aegypti in 1996 and the first autochthonous cases of dengue fever were registered in March 1998 1 . Since then, dengue fever cases were diagnosed in the following years with the isolation of dengue viruses (DENV) 1, 2, 3, and 4 in 2008 2 . In the present work, all 4 serotypes of the virus were detected in naturally infected A. aegypti by reverse transcription-polymerase chain reaction (RT-PCR), the amplicon sequence of DENV-4 was analyzed, and these findings were corroborated with the detection of DENV-4 in clinical samples of patients residing in the northern zone of Manaus, AM.A total of 187 mosquitoes were collected with BioGents (BG)-Sentinel traps 3 from January 2009 to June 2010 in 15 urban residential areas in the northern zone of Manaus, AM, Brazil. Sixty BG-Sentinel traps (4 traps in each of the 15 areas) were installed in each area every 2 weeks for a 24-h time period. The captured mosquitoes were identified and sexed. Cephalothoraxes of A. aegypti were separated for each area and each collection date in 2-mL Eppendorf cups (pool size: 1-10 mosquitoes) and maintained at -70°C until analysis.The pooled samples were pulverized and macerated on ice with 300mL Trizol reagent (Invitrogen,Brazil). Viral RNA extraction was performed according to the manufacturer's instructions and reverse transcription was performed using Superscript III (Invitrogen, Brazil) and random primers. Based on the previously described protocol 4 , semi-nested PCR was conducted to amplify a region of the viral genome that contains the capsid-premembrane (C-prM) genes, with primers designed to amplify 119-bp DENV-2, 290-bp DENV-3, and 392-bp DENV-4. The generated amplicon of DENV-4 was sequenced in both directions using the BigDye Terminator cycle sequence kit (Applied Biosystems, Foster City, CA, USA).Of the total of 187 pools of Aedes aegypti that were processed between August 2009 and July 2010, which each contained 10 females, 1 pool was positive for DENV-1, 4 pools were positive for DENV-2, 3 were positive for DENV-3, and 2 were positive for DENV-4. One pool was co-infected with DENV-2 and 3. The obtained sequence DENV-4 was submitted to a basic local alignment search tool (BLAST; http://www.ncbi.nlm.nih.gov.entrez) analysis, which uses the megablast algorithm optimized for ...

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Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil

Cited by 37 publications

References 19 publications

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Co-Infection of Dengue Virus by Serotypes 1 and 4 in Patient From Medium Sized City From Brazil

Clinical Profile of Concurrent Dengue Fever and Plasmodium vivax Malaria in the Brazilian Amazon: Case Series of 11 Hospitalized Patients

Identification of dengue viruses in naturally infected Aedes aegypti females captured with BioGents (BG)-Sentinel traps in Manaus, Amazonas, Brazil

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