Identification of a genetic marker associated with the resistance to Schistosoma mansoni infection using random amplified polymorphic DNA analysis

Abdel-Hamid, Abdel-Hamid Z.; Rawi, Sayed M.; Arafa, Amany A.

doi:10.1590/s0074-02762006000800007

Cited by 18 publications

(19 citation statements)

References 20 publications

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“…Some researcher used the same method (e.g. Oliveira et al, 2010) but others used the alternative polyacrylamide gel electrophoresis method (Abdel-Hamid et al, 2006;Oliveira et al, 2008;Spatz et al, 2000;Teixeira et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Dajem¹,

Ibrahim²,

Al‐Quraishy³

et al. 2011

Gene

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Section: Discussionmentioning

confidence: 99%

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Dajem¹,

Ibrahim²,

Al‐Quraishy³

et al. 2011

Gene

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“…The RAPD-PCR products were analyzed using agarose gel, which represents an effective and cheap method [33, 35]. However, other authors have used the alternative polyacrylamide gel electrophoresis method [21, 33, 36]. …”

Section: Discussionmentioning

confidence: 99%

“…The primers OPA-01, OPA-02, OPA-06, and OPM-04 have been used in previous studies in which specific polymorphic markers were detected between resistant and susceptible strains of different snail species to S. mansoni infection. They were used in B. glabrata [24, 26, 31, 32], B. tenagophila [25], in both B. glabrata and B. tenagophila [33], and B. alexandrina [21]. To our knowledge, the remaining random primers had not yet been tried in Biomphalaria spp .…”

Section: Methodsmentioning

confidence: 99%

“…They were reconstituted by adding sterile water to a final concentration of 100 pmoL/ μ L distributed in aliquots and stored at −20°C. The amplification conditions were as follows [21]: the tubes were transferred to the thermal cycler where they were subjected to one cycle of initial denaturation at 95°C for five minutes, then 40 cycles of 95°C for one minute, 30°C for one minute, 72°C for one minute followed by a final extension cycle at 72°C for 10 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…In B. tenagophila , two dominant genes determine resistance [17]. The susceptibility and resistance of B. alexandrina , as with other Biomphalaria spp ., were found to be heritable characteristics, with the resistance trait being dominant [16, 21]. However, the number of genes that determine this characteristic has not yet been identified.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Variation betweenBiomphalaria alexandrinaSnails Susceptible and Resistant toSchistosoma mansoniInfection

el-Nassery

Abou-El-Naga

Allam

et al. 2013

BioMed Research International

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Much effort has been made to control schistosomiasis infection in Egypt. However, enduring effects from such strategies have not yet been achieved. In this study, we sought to determine the genetic variability related to the interaction between Biomphalaria alexandrina snails and Schistosoma mansoni. Using RAPD-PCR with eight (10 mers) random primers, we were able to determine the polymorphic markers that differed between snails susceptible and resistant to Schistosoma mansoni infection using five primers out of the eight. Our results suggest that the RAPD-PCR technique is an efficient means by which to compare genomes and to detect genetic variations between schistosomiasis intermediate hosts. The RAPD technique with the above-noted primers can identify genomic markers that are specifically related to the Biomphalaria alexandrina/Schistosoma mansoni relationship in the absence of specific nucleotide sequence information. This approach could be used in epidemiologic surveys to investigate genetic diversity among Biomphalaria alexandrina snails. The ability to determine resistant markers in Biomphalaria alexandrina snails could potentially lead to further studies that use refractory snails as agents to control the spread of schistosomiasis.

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Biomphalaria alexandrina in Egypt: Past, present and future

Abou-El-Naga

2013

J Biosci

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The African species of Biomphalaria appeared as a result of the relatively recent west-to-east trans-Atlantic dispersal of the Biomphalaria glabrata-like taxon. In Egypt, Biomphalaria alexandrina is the intermediate host for Schistosoma mansoni. Biomphalaria alexandrina originated in the area between Alexandria and Rosetta and has historically been confined to the Nile Delta. Schistosoma mansoni reached Egypt via infected slaves and baboons from the Land of Punt through migrations that occurred as early as the Vth Dynasty. The suggestion of the presence of Schistosoma mansoni infection in Lower Egypt during Pharaonic times is discussed despite the fact that that there is no evidence of such infection in Egyptian mummies. It is only recently that Biomphalaria alexandrina colonized the Egyptian Nile from the Delta to Lake Nasser. This change was likely due to the construction of huge water projects, the development of new water resources essential for land reclamation projects and the movement of refugees from the Suez Canal zone to the Delta and vice versa. The situation with respect to Biomphalaria in Egypt has become complicated in recent years by the detection of Biomphalaria glabrata and a hybrid between both species; however, follow-up studies have demonstrated the disappearance of such species within Egypt. The National Schistosoma Control Program has made great strides with respect to the eradication of schistosoma; however, there has unfortunately been a reemergence of Schistosoma mansoni resistant to praziquantel. There are numerous factors that may influence the prevalence of snails in Egypt, including the construction of water projects, the increase in reclaimed areas, global climate change and pollution. Thus, continued field studies in addition to the cooperation of several scientists are needed to obtain an accurate representation of the status of this species. In addition, the determination of the genome sequence for Biomphalaria alexandrina and the use of modern technology will allow for the study of the host-parasite relationship at a molecular level.

show abstract

Identification of a genetic marker associated with the resistance to Schistosoma mansoni infection using random amplified polymorphic DNA analysis

Cited by 18 publications

References 20 publications

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Fingerprint of Biomphalaria arabica, the intermediate host of Schistosoma mansoni in Saudi Arabia, using RAPD-PCR

Genetic Variation betweenBiomphalaria alexandrinaSnails Susceptible and Resistant toSchistosoma mansoniInfection

Biomphalaria alexandrina in Egypt: Past, present and future

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