Cell proliferation and interferon-gamma response to recombinant MBP-3, NarL, MT-10.3, and 16kDa Mycobacterium tuberculosis antigens in Brazilian tuberculosis patients

Abstract

Human pulmonary tuberculosis (TB) is a worldwide public health problem. In resistant individuals, control of the infection mainly requires development of a Th1 cell immune response with production of cytokines, of which interferon-γ (IFN-γ)plays an important role. Several antigens from Mycobacterium tuberculosis complex has been described for use in vaccine development or for diagnostic purposes, however little evaluation has been done in endemic area for TB. The proliferative and IFN-γ human T cell immune responses, to four recombinant proteins NarL, 16 kDa) and PPD, of 38 Brazilian TB patients (6 untreated and 32 treated) Mycobacterium tuberculosis is an extremely successful pathogen infecting a third of the world's population, the cases being concentrated in developing countries (WHO 2002, Guzmán et al. 2003. Koch bacillus, which is transmitted by respiratory route from person to person, invades macrophage, multiply and can remain latent within the lung granuloma for years. Disease control is based on early identification of infected individuals followed by appropriate treatment. Disease progression prevention through inducement of protective immunity may be achieved through vaccination; however the current available Bacillus Calmette-Guérin (BCG) efficacy is controversial (Colditz et al. 1994, Fine 1995 recognition. However, little is known about the reactivity to these antigens in patients from Rio de Janeiro where are described the highest rates of tuberculosis (TB) incidence in Brazil (Hijjar 2005). The 16 kDa molecule is a polypeptide belonging to the α-cristallin family of low molecular weight, heat shock proteins, of which the coding gene (Rv2031c) has been found exclusively in the M. tuberculosis complex (Yuan et al. 1998). It has been reported as dominant protein produced in the static growth phase or under oxygen deprivation and required for bacterial replication inside macrophages (Yuan et al. 1998, Agrewala & Wilkinson 1998. MBP-3 is a 23 kDa maltose binding protein found as residue in the purification process of the MPT-64 antigen. The MT-10.3 or TB10.3 (Rv3019c) antigen, together with TB12.9 and TB10.4, comprise one subfamily within the esat-6 gene family and seem to be expressed during the TB infection (Skjot et al. 2002, Demissie et al. 2006. Two regulatory system components are ubiquitously distributed among bacteria and plants and are involved in the organisms' virulence (Urao et al. 2000). Nar L is the product of the response regulator narL gene of M. tuberculosis regulatory system (Parish et al. 2003).In the present study, we evaluated the production of interferon-γ (IFN-γ) and the proliferative response of peripheral blood mononuclear cells (PBMC), from TB patients from Rio de Janeiro, stimulated with the 858 858 858 858 858Immune response to mycobacterial antigens • Ricardo Candido Oliveira Tavares et al.following M. tuberculosis recombinant proteins: 16kDa, MBP-3, MT-10.3, and NarL. MATERIALS AND METHODSStudy subject -TB diagnosis was performed at district Hospital Souza Aguiar (H...

Assessment of the IgA Immunoassay Diagnostic Potential of the Mycobacterium tuberculosis MT10.3-MPT64 Fusion Protein in Tuberculous Pleural Fluid

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Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis

“…A series of clinical and experimental studies have demonstrated the importance of IFN- γ production in the control of tuberculosis [63–65]. Experiments in mice revealed that IFN- γ is an essential cytokine for macrophage activation and mycobacteria death in the intracellular environment.…”

The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients

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