Antifungal activity of propolis extract against yeasts isolated from onychomycosis lesions

Oliveira, Ana Carla Pozzi; Shinobu, Cristiane Suemi; Longhini, Renata; Franco, Selma Lucy; Svidzinski, Terezinha Inez Estivalet

doi:10.1590/s0074-02762006000500002

Cited by 83 publications

(75 citation statements)

References 20 publications

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“…[2][3][4][5][6] As propolis has characteristics that are beneficial to human health, such as antioxidant, anti-inflammatory, antifungal and anticancer activities, its chemical composition and properties have been extensively studied. [7][8][9][10] The antioxidant activity of ethanolic extracts of propolis has been evaluated by free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) scavenging, 8 by the ferric reducing ability power (FRAP) using the ferric-tripyridyltriazine (Fe 3+ -TPTZ) complex 11 and by oxidation of linoleic acid. 5 Similarly, mass spectrometry analyses has been used to examine the chemical composition of propolis extracts and its correlation with type and properties.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity and composition of propolis obtained by different methods of extraction

Cottica¹,

Sawaya²,

Eberlin³

et al. 2011

J. Braz. Chem. Soc.

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Extratos etanólicos de própolis obtidos a partir de diferentes concentrações de própolis e com álcool hidratado com diferentes teores de álcool/água foram analisados. A ação antioxidante destes extratos foi determinada através da captura do radical livre 1,1-difenil-2-picril-hidrazila (DPPH•) e do poder de redução férrico (FRAP) utilizando o complexo férrico-tripiridiltriazina (Fe 3+ -TPTZ). O teor de compostos fenólicos totais e a quantificação de flavonóides também foram verificados e obtidos por espectrometria de massas com ionização por electrospray (ESI(-)-MS) dos extratos. Observou-se que a composição química dos extratos de própolis e sua atividade antioxidante variam com a concentração de própolis e, principalmente, com o teor de álcool/água do etanol hidratado utilizado na extração. Foi possível perceber também que a atividade antioxidante dos extratos é significativamente dependente destes parâmetros de extração e que as composições de compostos fenólicos e flavonóides também variam muito. Os valores de FRAP não apresentaram, porém, boa correlação com o teor de flavonóides.Ethanolic extracts of propolis obtained with different concentrations of propolis and made using hydrous ethanol with various water/ethanol contents were analyzed. The antioxidant action of the extracts was determined by free radical 1,1-diphenyl-2-picryl-hydrazyl (DPPH•) scavenging and by ferric reducing ability power (FRAP), using the ferric-tripyridyl triazine (Fe 3+ -TPTZ) complex. The total phenolic and flavonoid contents were also determined, and the electrospray ionization mass spectrometry spectra (ESI(-)-MS) of the extracts were acquired. The chemical composition of propolis extracts and their antioxidant activity were observed to vary with the concentration of propolis, and especially with the water/ethanol contents of the hydrous ethanol used for extraction. The antioxidant activity of the extracts was also found to be significantly dependent on these extraction parameters, as well as on the composition of the phenolic compounds and flavonoids. FRAP values showed, however, poor correlation with the flavonoid content. Keywords: total phenolic contents, antioxidant activity, ESI(-)-MS fingerprint, propolis extraction, flavonoids IntroductionPropolis is a resin produced by honey bees by mixing wax, pollen, salivary secretions and collected natural resins. 1 The chemical composition of propolis is complex and varies with the vegetation visited by bees, as well as with bee species. [2][3][4][5][6] As propolis has characteristics that are beneficial to human health, such as antioxidant, anti-inflammatory, antifungal and anticancer activities, its chemical composition and properties have been extensively studied. [7][8][9][10] The antioxidant activity of ethanolic extracts of propolis has been evaluated by free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) scavenging, 8 by the ferric reducing ability power (FRAP) using the ferric-tripyridyltriazine Antioxidant Activity and Composition of Propolis Obtained by Different Methods o...

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Section: Introductionmentioning

confidence: 99%

Antioxidant activity and composition of propolis obtained by different methods of extraction

Cottica¹,

Sawaya²,

Eberlin³

et al. 2011

J. Braz. Chem. Soc.

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“…(Soares et al, 2006;Tavares et al, 2006). Entre outras, está comprovada a ação antimicrobiana frente a vários agentes etiológicos, por exemplo, bactérias Gram positivas como Streptococcus pyogenes, Staphylococcus aureus e Gram negativas Escherichia coli (Burdock, 1998;Nieva, 1999;Kujumgiev, 1999;Packer;Luz, 2007) e leveduras, sobretudo Candida albicans (Fernandes et al, 1995;De Castro, 2001, Oliveira et al, 2006. Atualmente, há estudos sobre a própolis nas mais diversas áreas, desde a caracterização da droga (Franco et al, 2000;Bruschi et al, 2003) e otimização dos processos de extração Bueno, 1999) até controle de qualidade (Sousa et al 2007).…”

Section: Introductionunclassified

Obtenção de extratos de própolis sob diferentes condições e avaliação de sua atividade antifúngica

Longhini¹,

Raksa²,

Oliveira³

et al. 2007

Rev. bras. farmacogn.

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“…Martinez Garcia et al (14) ;Duailibe et al (15) Clinical -Antimicrobial action Azevedo et al (4) ;Santos et al (11) ; Lofuto et al (35) Clinical -Therapeutic action in wound healing Peruchi et al (2) ;Park et al (3) ;Vargas et al (5) ; Fernandes Júnior et al (6) ; Silva et al (7) ;Sforcin et al (8) ; Sforcin et al (9) ;Oliveira et al (10) ; Auricchio et al (35) ; Fernandes Júnior et al (17) ;Machado et al (18) ;Longhini et al (19) ;Sawaya et al (20) ;Fernandes et al (21) ;Fernandes Júnior et al ( 22) ; Muli et al (23) ;Gebara et al (24) ;Fernandes Júnior et al (25) ;Bianchini et al (26) ;Gonçales et al (27) ;Dantas et al (28) ; Ayres et al (29) ;Orsi et al (30) ;Orsi et al (31) ;Sforcin et al (32) ;Sforcin et al (33) ;Orsi et al (34) ; Fernandes Júnior et al (36) ;Fernandes et al (37) ; Araújo et al (38) Experimental -Antimicrobial action Rahal et al (16) Experimental -Therapeutic action in wound healing (1) . Another study, which used propolis ointment on 22 chronic wounds, of which 11 were venous ulcers, seven pressure ulcers, two diabetic ulcers, and two post-trauma injuries, found that necrotic tissue was present in 14 (8.2%) during tissue assessment process.…”

Section: Thematic Focus Approachmentioning

confidence: 99%

“…These properties are found to be associated with its chemical composition, which has, until this moment, about 200 compounds already identified, of which the main groups are flavonoids, fat acids, alcohols, amino acids, vitamins and minerals (2,(4)(5)(6)(7)(8)(9)(10)(11) .…”

Section: Introductionmentioning

confidence: 99%

Ação terapêutica da própolis em lesões cutâneas

et al. 2009

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This integrative literature review aimed to analyze the findings of studies that focused on the therapeutic properties of propolis for treatment of skin lesions. Studies were obtained through literature searches using LILACS, MEDLINE, and BDENF databases. The search was limited to publications in Portuguese, English, or Spanish languages from 1980 to 2007. Initially, 1127 publications were identified. However, only 38 publications met the study inclusion criteria. Seven publications (18.42%) were clinical studies and remainder of the 31 publications (81.58%) used "in vitro" or with animal experiments. These publications were focused on the therapeutic properties of propolis in wound healing and on the antimicrobial properties of propolis. Findings suggested the effectiveness of propolis for treating skin lesions, acting as a natural healing and antimicrobial agent whose therapeutic properties depends on the form and place of extraction and concentration of the product. Keywords: Propolis/therapeutic use; Wound healing/nursing RESUMO Trata-se de uma revisão integrativa da literatura que teve como objetivo analisar os estudos que abordam o uso terapêutico da própolis em lesões cutâneas. Os dados foram obtidos por meio de busca nas bases de dados LILACS, MEDLINE, e BDENF, abrangendo o período de 1980 a 2007, nos idiomas português, inglês e espanhol. Identificaram-se 1127 artigos, dos quais 38 atenderam aos critérios de inclusão deste estudo. Destes artigos, 7 (18,4%) eram estudos clínicos e 31 (81,6%) experimentais "in vitro" e em animais. Quanto à abordagem, foram classificados em dois focos temáticos: atuação terapêutica da própolis na cicatrização das lesões e ação antimicrobiana da própolis. A análise dos trabalhos destacou a eficácia da própolis no tratamento de feridas, atuando como agente cicatrizante e antimicrobiano natural, cujas propriedades dependem diretamente da forma e local de extração e concentração do produto. Descritores: Própole/uso terapêutico; Cicatrização de feridas/enfermagem RESUMEN Se trata de una revisión integradora de la literatura con el objetivo de analizar los estudios que abordan el uso terapéutico del própolis en lesiones cutáneas. Los datos fueron obtenidos por medio de la búsqueda en las bases de datos LILACS, MEDLINE, y BDENF, abarcando el período de 1980 al 2007, en los idiomas portugués, inglés y español. Se identificaron 1127 artículos, de los cuales 38 atendieron a los criterios de inclusión del estudio. De estos artículos, 7 (18,4%) eran estudios clínicos y 31 (81,6%) experimentales "in vitro" y en animales. En cuanto al abordaje, fueron clasificados en dos focos temáticos: actuación terapéutica del própolis en la cicatrización de las lesiones y acción antimicrobiana del própolis. El análisis de los trabajos destacó la eficacia del própolis en el tratamiento de heridas, actuando como agente cicatrizante y antimicrobiano natural, cuyas propiedades dependen directamente de la forma y local de extracción y concentración del producto. Descriptores: Própolis/uso terapéut...

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Antifungal activity of propolis extract against yeasts isolated from onychomycosis lesions

Cited by 83 publications

References 20 publications

Antioxidant activity and composition of propolis obtained by different methods of extraction

Antioxidant activity and composition of propolis obtained by different methods of extraction

Obtenção de extratos de própolis sob diferentes condições e avaliação de sua atividade antifúngica

Ação terapêutica da própolis em lesões cutâneas

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