A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)

Camargo, Maurício; Duque-Correa, María A.; Berrío, Alejandro

doi:10.1590/s0074-02762006000300021

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…Cytogenetic studies have significantly contributed to our understanding of the taxonomy and systematics of insects, despite the small size of the chromosomes and their holocentric nature, which hinder in the detection of their rearrangement or changes in the structures (Camargo et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector

Alevi¹,

Mendonca²,

Succi³

et al. 2012

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector

Alevi¹,

Mendonca²,

Succi³

et al. 2012

Genet. Mol. Res.

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“…This protocol combines controlled mechanical scattering, micro-centrifugation, and micro-spreading steps of smaller amounts (microliters) of male gonads. Using a single centrifugation microtube, excellent cytogenetic preparations from insect species, such as the reduviid kissing bug, Rhodnius prolixus Stål, 1859 and Triatoma lecticularia (Stål 1859) have been generated (Camargo et al 2006). This method overcomes some of the drawbacks of the classical squash method (Camargo et al 2006).…”

Section: Some Cytogenetic Methods For the Investigation Of Insect Chrmentioning

confidence: 99%

“…Using a single centrifugation microtube, excellent cytogenetic preparations from insect species, such as the reduviid kissing bug, Rhodnius prolixus Stål, 1859 and Triatoma lecticularia (Stål 1859) have been generated (Camargo et al 2006). This method overcomes some of the drawbacks of the classical squash method (Camargo et al 2006). Also, the technique of micro-centrifugation and micro-spreading has several additional advantages: a. many slides per gonad can be obtained, On the other hand, as far as we are aware, this technique has not been standardized for use in other species.…”

Section: Some Cytogenetic Methods For the Investigation Of Insect Chrmentioning

confidence: 99%

“…As a main problem in entomological studies is the difficulty in identifying closely related species (Okiwelu and Noutcha 2014), chromosomal data have provided ample opportunities for taxonomic and phylogenetic analyses of many insect groups (Miao andHua 2017, Soldán andPutz 2000). Cytogenetic studies have detected fundamental patterns in chromosome evolution that have assisted in the systematic analyses of many taxa (Camargo et al 2006).…”

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confidence: 99%

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Some Cytogenetic Methods for the Investigation of Insect Chromosomes and Their Implications for Research in Systematic Entomology

Koçak¹,

Okutaner²

2017

LEB

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Abstract:The use of karyological studies in insects is of considerable significance. However, chromosome karyotyping has been hampered principally by: 1) the minute size of many insects, 2) the recovery of suitable tissues, 3) the small number of cells adequate for analyses 4) the smallness of the chromosomes in some groups, 5) the difficulty in obtaining satisfactory numbers of well-spread or dispersed metaphases, and 6) the determination of the best time of the year to carry out chromosome preparations using freshly collected material because the life history of many insects is still not well-known. Many attempts have been made to reduce these difficulties by developing novel karyological methods. These procedures can yield chromosome preparations applicable to a wide variety of insects. In this paper, we outline techniques for preparation and observation of chromosomes from insect cells. Also, implications of chromosome techniques on insect systematics are discussed.

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Chromosomal-Level Genome Assembly of the Sea Urchin Lytechinus variegatus Substantially Improves Functional Genomic Analyses

Davidson

Guo

Wang

et al. 2020

Genome Biology and Evolution

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Lytechinus variegatus is a camarodont sea urchin found widely throughout the western Atlantic Ocean in a variety of shallow-water marine habitats. Its distribution, abundance, and amenability to developmental perturbation make it a popular model for ecologists and developmental biologists. Here, we present a chromosomal-level genome assembly of L. variegatus generated from a combination of PacBio long reads, 10X Genomics sequencing, and HiC chromatin interaction sequencing. We show L. variegatus has 19 chromosomes with an assembly size of 870.4 Mb. The contiguity and completeness of this assembly is reflected by a scaffold length N50 of 45.5Mb and BUSCO completeness score of 95.5%. Ab-initio and transcript-informed gene modelling and annotation identified 27,232 genes with an average gene length of 12.6 Kb, comprising an estimated 39.5% of the genome. Repetitive regions, on the other hand, make up approximately 45.4% of the genome. Physical mapping of well-studied developmental genes onto each chromosome reveals non-random spatial distribution of distinct genes and gene families, which provides insight into how certain gene families may have evolved and are transcriptionally regulated in this species. Lastly, aligning RNA-seq and ATAC-seq data onto this assembly demonstrates the value of highly contiguous, complete genome assemblies for functional genomics analyses that is unattainable with fragmented, incomplete assemblies. This genome will be of great value to the scientific community as a resource for genome evolution, developmental, and ecological studies of this species and the Echinodermata.

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A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)

Cited by 3 publications

References 7 publications

Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector

Karyotype and spermatogenesis in Triatoma lenti (Hemiptera: Triatominae), a potential Chagas vector

Some Cytogenetic Methods for the Investigation of Insect Chromosomes and Their Implications for Research in Systematic Entomology

Chromosomal-Level Genome Assembly of the Sea Urchin Lytechinus variegatus Substantially Improves Functional Genomic Analyses

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