Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence

Vargas, Erika; Espitia, Claudia; Patiño, Carlos Luis Iglesias; Pinto, Néstor; Aguilera, Germán; Bargues, M. D.; Guhl, Felipe

doi:10.1590/s0074-02762006000100008

Cited by 25 publications

(8 citation statements)

References 15 publications

(17 reference statements)

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“…In our study, 17% of T. venosa collected were nymphs, and 71.4% were collected in intradomiciliary areas. Considering that previously ecoepidemiological studies in Boavita (Boyaca) showed high mobility of species between peridomicile and intradomiciliary environments [45], the results in the current study could be a consequence of T. venosa mobility in the peridomicile area or a result of a peculiar domiciliation of this triatomine species in this department [9,17]. Therefore, we suggest that both scenarios should be explored.…”

Section: Discussionmentioning

confidence: 74%

Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the Highlands of Colombia

et al. 2021

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Introduction Updating the distribution and natural infection status of triatomine bugs is critical for planning, prioritizing, and implementing strategies to control Chagas disease (CD), especially after vector reduction programs. After carrying out a control program, the Department of Boyaca contains the highest number of Colombian municipalities certified by PAHO to be free of intradomiciliary transmission of Trypanosoma cruzi by Rhodnius prolixus. The present work describes the spatial distribution, natural infection (NI), and molecular characterization of T. cruzi in synanthropic triatomines from the Department of Boyaca in 2017 and 2018. Materials and methods An entomological survey was conducted in 52 municipalities in Boyaca known to have had previous infestations of triatomine bugs. Insects were collected through active searches carried out by technical personnel from the Secretary of Health and community members using Triatomine Collection Stations (PITs-acronym in Spanish). For evaluation of natural infection, triatomines were identified morphologically and grouped in pools of one to five individuals of the same species collected in the same household. DNA derived from the feces of each pool of insects was analyzed by PCR for the presence of T. cruzi using primers flanking the satellite DNA of the parasite. SL-IR primers were used to differentiate TCI from the other DTUs and to identify different genotypes. The distribution of the collected triatomines was analyzed to determine any vector hotspots using spatial recreation. Results A total of 670 triatomine bugs was collected, belonging to five species: Triatoma dimidiata (73.2%), Triatoma venosa (16.7%), Panstrongylus geniculatus (5.7%), Rhodnius prolixus (4.4%), and Panstrongylus rufotuberculatus (0.4%), from 29 of the 52 municipalities. In total, 71.6% of the bugs were collected within houses (intradomiciliary) and the rest around the houses (peridomiciliary). Triatoma dimidiata was the most widely distributed species and had the highest natural infection index (37.8%), followed by T. venosa and P. geniculatus. TcI was the only DTU found, with the TcI Dom genotype identified in 80% of positive samples and TcI sylvatic in the other insects. Spatial analysis showed clusters of T. dimidiata and T. venosa in the northeast and southwest regions of Boyaca. Conclusions After some municipalities were certified free of natural transmission within houses (intradomiciliary transmission) of T. cruzi by R. prolixus, T. dimidiata has become the most prevalent vector present, and represents a significant risk of resurgent CD transmission. However, T. venosa, P. geniculatus, and P. rufotuberculatus also contribute to the increased risk of transmission. The presence of residual R. prolixus may undo the successes achieved through vector elimination programs. The molecular and spatial analysis used here allows us to identify areas with an ongoing threat of parasite transmission and improve entomological surveillance strategies.

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Section: Discussionmentioning

confidence: 74%

Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the Highlands of Colombia

et al. 2021

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“…Because the statistical analyses of genetic variability expressed by morphological characters are a good measure of population differentiation (Patterson et al, 2001), morphometrics is an efficient alternative method for taxonomic studies of Triatominae (Dujardin et al, 1999;Matias et al, 2001;Jaramillo et al, 2002;Villegas et al, 2002;Gumiel et al, 2003, Lehmann et al, 2005Vargas et al, 2006;Feliciangeli et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Misidentification of two Brazilian triatomes, Triatoma arthurneivai and Triatoma wygodzinskyi, revealed by geometric morphometrics

Carbajal-de-la-Fuente

Jaramillo

Barata

et al. 2010

Medical and Veterinary Entomology

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Triatoma arthurneivai Lent & Martins and Triatoma wygodzinskyi Lent (Hemiptera: Reduviidae) are two Brazilian species found in the sylvatic environment. Several authors may have misidentified T. arthurneivai and consequently published erroneous information. This work reports the use of geometric morphometric analysis on wings in order to differentiate T. arthurneivai and T. wygodzinskyi, and thus to detect possible misidentifications. Triatomines collected from the field in the states of Minas Gerais and São Paulo, and from laboratory colonies, were used. Analyses show a clear differentiation between specimens of T. arthurneivai and T. wygodzinskyi. This indicates that T. arthurneivai populations from São Paulo state were misidentified and should be considered as T. wygodzinskyi. This study also suggests that T. arthurneivai is an endemic species from Serra do Cipó, Minas Gerais state.

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“…Todas estas particularidades del hábitat, parecen afectar de un modo específico las características morfofisiológicas de T. infestans resultando en cambios que serían de utilidad para la identificación de insectos recolonizantes o con diferentes capacidades dispersivas (Schachter-Broide et al, 2004;Abrahan et al, 2008;Hernández et al, 2011;Hernández et al, 2013). Diversas son las investigaciones que dan cuenta del uso la técnica de morfometría geométrica y fenotipos antenales en triatominos para análisis de dispersantes (Dumonteil et al, 2007), estructura poblacional (Vargas et al, 2006;Schachter-Broide et al, 2004;Hernández et al, 2011;Gaspe et al, 2012) características fenéticas compatibles con vuelo, entre otros (Hernández et al, 2015;Hernández et al, 2020). El análisis de la estructuración espacial poblacional permite determinar la continuidad que puede existir en la distribución geográfica de las poblaciones de una especie, mediante la caracterización de sus individuos y la comparación de variables relacionadas al fenotipo, al genotipo, o ambas (Dujardin et al, 2002).…”

Section: Introductionunclassified

Efecto de la vigilancia Entomológica en la estructuración poblacional de triatoma infestans (hemiptera, reduviidae) de intra y peridomicilio en un área endémica de la Provincia de la Rioja (Argentina).

Hernández

2020

Rev. Fac. Cienc.

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Se analiza aquí, la influencia del rociado y vigilancia sobre la estructuración de las poblaciones peri e intradomésticas de T. infestans usando morfología cuantitativa. Las colectas en intra (ID) y peridomicilio (PD) se realizaron sobre una población libre de rociado por 10 años y en poblaciones hasta 8 años post rociado (población con vigilancia y rociado). En pre-rociado, no se observó estructuración poblacional entre ID y PDPost intervención, se detectaron 6 adultos de T. infestans en ID. Estos insectos mostraron similitud morfológica con T. infestans residuales. Los resultados plantean que en presencia de vigilancia entomológica, los ID pueden permanecer libres de T. infestans aún con PD infestados.

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Genetic structure of Triatoma venosa (Hemiptera: Reduviidae): molecular and morphometric evidence

Abstract: Triatoma venosa presents a restricted geographical distribution in

Cited by 25 publications

References 15 publications

Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the Highlands of Colombia

Distribution and natural infection status of synantrophic triatomines (Hemiptera: Reduviidae), vectors of Trypanosoma cruzi, reveals new epidemiological scenarios for chagas disease in the Highlands of Colombia

Misidentification of two Brazilian triatomes, Triatoma arthurneivai and Triatoma wygodzinskyi, revealed by geometric morphometrics

Efecto de la vigilancia Entomológica en la estructuración poblacional de triatoma infestans (hemiptera, reduviidae) de intra y peridomicilio en un área endémica de la Provincia de la Rioja (Argentina).

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