Nitric oxide: a major determinant of mast cell phenotype and function

Abstract

Mast cells (MC)

Anti-allergic activity of German chamomile (Matricaria recutita L.) in mast cell mediated allergy model

“…Nitric oxide (NO) is a diatomic radical produced by nitric oxide synthase (NOS), and has cytotoxic properties. NO can influence many mast cell functions including adhesion, degranulation, early mediator release and leukotriene production (Mc Cauley et al, 2005). During immune and inflammatory allergy conditions, NO is generated at relatively high and sustained levels by the inducible form of NOS-2 (Coleman, 2002).…”

“…The rats were anaesthetized by anesthetic ether and then blood was collected from retro-orbital, centrifuged at 500 rpm for 5 min then equal volume of serum, Griess reagent (pH 2) was added and incubated for 15 min at 37 • C. The normal group sample was incubated with normal saline, control and extract treated groups were incubated with compound 48/80 (1 g/ml) and after completion of incubation period, the absorbance was measured at 546 nm (Mc Cauley et al, 2005;Ganapaty et al, 2010).…”

“…The equal volume of peritoneal fluid, Griess reagent (pH 2) and 40 l of glycine buffer was added and incubated for 15 min at 37 • C, then the incubated sample was centrifuged and the supernatant was used for measurement of absorbance. The normal group sample was incubated with normal saline, control and extract treated groups were incubated with compound 48/80 (1 g/ml) at 37 • C for 15 min and the absorbance was measured at 546 nm (Mc Cauley et al, 2005).…”

Protein Tyrosine Nitration of Aldolase in Mast Cells: A Plausible Pathway in Nitric Oxide-Mediated Regulation of Mast Cell Function

“…Reactive oxygen species and reactive nitrogen species are important in the regulation of MC secretion and in inflammatory conditions (4). Several studies demonstrated that MCs express NO synthases and can produce small amounts of NO (5)(6)(7)(8)(9)(10)(11). Moreover, NO from other sources, such as macrophages, can regulate MC activities (12).…”

“…Moreover, NO from other sources, such as macrophages, can regulate MC activities (12). Endogenous and exogenous NO can inhibit MC degranulation; protease release; adherence to fibronectin; and leukotriene, cytokine, and chemokine production (4,7). By contrast, NO can also upregulate MC properties, such as CD8 expression (13), cytotoxicity (14), and cyclooxygenase-2 expression (15).…”