Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli

Acosta, María Beatriz Riverón; Ferreira, Rita de Cássia Café; Ferreira, Luís Carlos S.; Costa, Samuel Macedo

doi:10.1590/s0074-02762005000700020

Cited by 11 publications

(9 citation statements)

References 28 publications

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“…OppA is an oligopeptide ABC transporter substrate‐binding protein that constitutes an oligopeptide permease transport system that is responsible for the transport of oligopeptides into cells (Wium, Botes, & Bellstedt, ). Aminoglycoside antibiotics were transferred into cells through the oligopeptide permease transport system, and the reduced expression of OppA was associated with aminoglycoside resistance (Acosta, Ferreira, Ferreira, & Costa, ). It has been reported that the oligopeptide transport system was important for the uptake of aminoglycoside antibiotics since a decrease in OppA was observed in approximately 40% of spontaneous aminoglycoside‐resistant mutant (Kashiwagi et al, ).…”

Section: Discussionmentioning

confidence: 99%

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

et al. 2019

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China launched the Tiangong‐2 space laboratory in 2016 and will eventually build a basic space station by the early 2020s. These spaceflight missions require astronauts to stay on the space station for more than 6 months, and they inevitably carry microbes into the space environment. It is known that the space environment affects microbial behavior, including growth rate, biofilm formation, virulence, drug resistance, and metabolism. However, the mechanisms of these alternations have not been fully elucidated. Therefore, it is beneficial to monitor microorganisms for preventing infections among astronauts in a space environment. Salmonella enteritidis is a Gram‐negative bacterial pathogen that commonly causes acute gastroenteritis in humans. In this study, to better understand the effects of the space environment on S. enteritidis, a S. enteritidis strain was taken into space by the Shenzhou‐11 spacecraft from 17 October 2016 to 18 November 2016, and a ground simulation with similar temperature conditions was simultaneously performed as a control. It was found that the flight strain displayed an increased growth rate, enhanced amikacin resistance, and some metabolism alterations compared with the ground strain. Enrichment analysis of proteome revealed that the increased growth rate might be associated with differentially expressed proteins involved in transmembrane transport and energy production and conversion assembly. A combined transcriptome and proteome analysis showed that the amikacin resistance was due to the downregulation of the oppA gene and oligopeptide transporter protein OppA. In conclusion, this study is the first systematic analysis of the phenotypic, genomic, transcriptomic, and proteomic variations in S. enteritidis during spaceflight and will provide beneficial insights for future studies on space microbiology.

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Section: Discussionmentioning

confidence: 99%

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

et al. 2019

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“…Intracellular polyamine pools affect, at the posttranscriptional level, OppA expression and the action of aminoglycosides in E. coli K12 (Goldemberg & Algranati, 1981; Acosta et al , 2005). Indeed, addition of polyamines to the growth medium enhances the sensitivity of E. coli K12 strains to aminoglycosides, while low intracellular polyamine pools may reduce OppA expression and aminoglycoside sensitivity (Kashiwagi et al , 1998; Acosta et al , 2005). Although the role of polyamines in OppA expression has been clearly established (Igarashi et al , 1997), further studies are required to elucidate the impact of polyamines on the sensitivity to aminoglycosides.…”

Section: Discussionmentioning

confidence: 99%

Oligopeptide uptake and aminoglycoside resistance in Escherichia coli K12

Nakamatsu

Fujihira

Ferreira

et al. 2007

FEMS Microbiology Letters

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Previous reports have suggested that Escherichia coli K12 mutants defective in the expression of oligogopeptide permease protein A (OppA) exhibit reduced sensitivity to aminoglycosides due to altered permeability of the cell envelope. In this work, the role of the OppA protein, and the oligogopeptide permease (Opp) transport system has been evaluated, in the resistance to aminoglycosides using derivatives of the E. coli K12 SS320 strain selected for triornithine resistance or with a deletion of the complete opp operon. All tested mutants were defective in the uptake of tri- and tetra-peptides but did not expressed resistance to aminoglycosides. Additionally, complementation tests carried out with a plasmid encoding the OppA protein did not affect the sensitivity of the strains to these antibiotics. Taken together, these evidences indicate that the Opp uptake system, as well as the OppA protein, does not play a direct role in the sensitivity to aminoglycosides in E. coli K12.

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“…Para tal, o modo de avaliação considerado padrão-ouro do papel fisiológico dos sistemas de transporte de nutrientes baseia-se no emprego de moléculas radioativamente marcadas, quando disponíveis, no qual é possível mensurar a incorporação destas, bem como avaliar as características bioquímicas do sistema de transporte (KRASTEL et al, 2010;RAKSAJIT et al, 2006). (ACOSTA et al, 2005;AMES et al, 1973;NAIDER et al, 1975;NAKAMATSU et al, 2007;RUDNER et al, 1991 Além da captação de oligopeptídeos ser um fator importante para a sobrevivência, seu papel fisiológico pode ser estendido, como observado em E. coli e S. Typhimurium, nas quais esse sistema é responsável pela reciclagem de até 50% da peptideoglicana da parede celular bacteriana (GOODELL et al, 1987;PARK et al, 1998). Em diversas bactérias gram-positivas, como Bacillus subtilis e Streptococcus pneumoniae, a capacidade de transformação se deve a respostas a comunicações intercelulares dependentes de peptídeos e que só ocorrem na presença de um sistema de captação de oligopeptídeos funcional, incluindo no mínimo um ortólogo de OppA (ALLOING et al, 1998;PEARCE et al, 1994;RUDNER et al, 1991).…”

Section: Figura 1-sistema De Transporte Abcunclassified

Análise do papel dos transportadores ABC de oligopeptídeos, poliaminas, fosfato inorgânico, glutamato e glutamina na fisiologia e patogênese de bactérias do trato gastro-intestinal.

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Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli

Abstract: The role of intracellular free polyamine (putrescine and spermidine)

Cited by 11 publications

References 28 publications

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

Increased growth rate and amikacin resistance of Salmonella enteritidis after one‐month spaceflight on China’s Shenzhou‐11 spacecraft

Oligopeptide uptake and aminoglycoside resistance in Escherichia coli K12

Análise do papel dos transportadores ABC de oligopeptídeos, poliaminas, fosfato inorgânico, glutamato e glutamina na fisiologia e patogênese de bactérias do trato gastro-intestinal.

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