Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

Vivoni, Adriana Marcos; Moreira, Beatriz Meurer

doi:10.1590/s0074-02762005000700001

Cited by 9 publications

(8 citation statements)

References 49 publications

(28 reference statements)

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“…Segundo a Organização Mundial da Saúde (OMS), a mortalidade nas maternidades se correlaciona negativamente com o funcionamento do sistema de saúde, entretanto, no Brasil a notificação da mortalidade nesses hospitais ainda é precária, exigindo um maior empenho das autoridades 18 . Em estudo retrospectivo, realizado entre 1927 a 2001, numa Maternidade Escola de Juiz de Fora, MG, a infecção puerperal representou 15,4% das causas de morte materna nesse local 1 .…”

Section: Resultsunclassified

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Prevalência de Staphylococcus spp resistentes à meticilina isolados em uma maternidade escola da Cidade de Natal, Estado do Rio Grande do Norte

Júnior

Nunes

Nascimento

et al. 2009

Rev. Soc. Bras. Med. Trop.

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Com o objetivo de determinar a prevalência de Staphylococcus spp resistentes à meticilina isolados na Maternidade Escola Januário Cicco, Natal, RN, no período de 2002 a 2003, analisou-se 1.576 materiais clínicos de pacientes hospitalizados. As amostras foram coletadas, processadas e identificadas conforme procedimento padrão para cada espécime clínico. O perfil de susceptibilidade in vitro foi realizado pelo método de Kirby-Bauer. Isolou-se 188 cepas de Staphylococcus spp, das quais 105 foram identificadas como Staphylococcus aureus e 83 como Staphylococcus coagulase negativos. Staphylococcus aureus foi isolado com mais freqüência em secreções enquanto Staphylococcus coagulase negativos foram mais prevalentes em hemoculturas. A elevada (41,5%) prevalência dos Staphylococcus spp resistentes à meticilina demonstra a necessidade de medidas profiláticas imediatas com o objetivo de impedir a disseminação desse fenômeno.

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Section: Resultsunclassified

“…microrganismos, a resistência é resultado do gene cromossômico mecA, que produz uma nova proteína ligadora de penicilina (PBP) com baixa afinidade pelos beta-lactâmicos 18 .…”

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Prevalência de Staphylococcus spp resistentes à meticilina isolados em uma maternidade escola da Cidade de Natal, Estado do Rio Grande do Norte

Júnior

Nunes

Nascimento

et al. 2009

Rev. Soc. Bras. Med. Trop.

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“…Many molecular methods have been employed to type, differentiate, and group isolates of S. aureus (Shopsin & Kreiswirth, 2001;Trindade et al, 2003;Robinson & Enright, 2004;Vivoni & Moreira, 2005). While currently pulsed-field gel electrophoresis (PFGE), usually with SmaIgenerated genomic DNA fragments, is considered the 'gold' standard method, it is time-consuming, requires specific and expensive equipment, and is relatively labour intensive (Arshad et al, 1993;Wagner & Lai, 1994).…”

Section: Introductionmentioning

confidence: 99%

Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis

Collery

Smyth

Twohig

et al. 2008

Journal of Medical Microbiology

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Forty-eight Staphylococcus aureus isolates collected from a young, healthy, Irish university student population from 1995 to 2004 were screened for 16 enterotoxin (SE) and enterotoxin-like (SEl) genes (sea–see, seg–sei, selj–selo, selq, selu), and for the toxic shock toxin syndrome toxin-1 gene, tst. All of the isolates harboured at least one SE or SEl gene and 66.7 % possessed a classical SE gene (sea, seb, sec), the commonest being the seb gene. Most of the isolates (85.4 %) had a complete egc locus (selo, selm, sei, seln, seg). The intergenic sei–seln region of the egc locus was typed by PCR-RFLP in 34 isolates, 15 possessing pseudogenes ψent1 and ψent2 and 19 having the selu gene. The seh and sell genes, the selk–selq gene combination, and the tst gene were each found in <15 % of isolates. The agr genotype distribution was agr type III, 37.5 %; agr type I, 35.4 %; agr type II, 25 %; and agr type IV, 2.1 %. There was no association between SE–SEl genotype and agr type. All tst gene-positive isolates were of agr type III and harboured a classical SE gene. Multiple locus, variable number tandem repeat analysis (MLVA) produced 47 different patterns. While the sdr locus was present in all isolates, half of them lacked one or two of the sdr gene amplimers. Twenty isolates harboured the bbp gene, its presence being associated with agr type III, but not with the SE–SEl gene profile. The agr types of isolates were associated with MLVA subclusters. Selective MLST analysis revealed seven novel sequence types and a new aroE allele. Five clonal clusters (CCs), including CCs comprising major pandemic clones CC30, CC5 and CC22 and minor lineages CC6 and CC9, and three singletons were identified.

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“…Staphylococcus is transient colonizer of skin and mucosal membranes of both humans and animals (Pantosti, 2012). Enterotoxin-producing S. aureus are recognized as the most frequent agents of the blood stream and skin infections (Vivoni and Moreira, 2005). Further, S. aureus is an important cause of pneumonia, postoperative wound infections as well as nosocomial infections (Lee, 2003).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic heterogeneity of Staphylococcus aureus strains isolated from food and clinical samples from northern Jordan using VNTR, toxin profiles and antibiograms

Jaradat¹,

Ababneh²,

Saraireh³

et al. 2016

MJM

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Aims: Staphylococcus aureus is a Gram positive pathogen distributed worldwide and represents a rising problem for both hospitals and community. The aims of the study were to examine the antibiograms, toxin profiles as well as the genetic diversity of a set of S. aureus isolates from clinical and food samples. Methodology and results: To get some insights on the genetic heterogeneity and test for the presence of certain virulence genes, all isolates were subjected to different PCR amplifications and antibiotic sensitivity analysis. The mecA gene was detected in both clinical and food isolates. Resistance to penicillin and amoxicillin was observed in both clinical and food isolates. About 88% of both food and clinical isolates harbored the toxin gene sea, while 70% and 29% of clinical and food isolates respectively, harbored sec. The seb gene was detected in 59% and 18% of clinical and food isolates, respectively. Dendrograms prepared from the VNTR, antibiograms and toxin profiles, revealed 89, 52 and 12 clusters, respectively. Thus, suggesting a very high heterogeneity among the isolates. Conclusion, significance and impact of study: Strains used in this study showed high heterogeneity when examined by VNTR or antibiograms, while appeared less heterogeneous when dendrogram was generated based on toxin profiles. This study highlights the fact that methicillin resistance in S. aureus might be generated within the health institutions or the community. Obtained results also might help health authorities understand the origin of methicillin resistant clones within the study area.

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Application of molecular techniques in the study of Staphylococcus aureus clonal evolution - A Review

Cited by 9 publications

References 49 publications

Prevalência de Staphylococcus spp resistentes à meticilina isolados em uma maternidade escola da Cidade de Natal, Estado do Rio Grande do Norte

Prevalência de Staphylococcus spp resistentes à meticilina isolados em uma maternidade escola da Cidade de Natal, Estado do Rio Grande do Norte

Molecular typing of nasal carriage isolates of Staphylococcus aureus from an Irish university student population based on toxin gene PCR, agr locus types and multiple locus, variable number tandem repeat analysis

Analysis of genetic heterogeneity of Staphylococcus aureus strains isolated from food and clinical samples from northern Jordan using VNTR, toxin profiles and antibiograms

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