Antileishmanial IgG and IgE antibodies recognize predominantly carbohydrate epitopes of glycosylated antigens in visceral leishmaniasis

Atta, Ajax Mercês; Colossi, R; Sousa-Atta, M.L.B.; Jerônimo, Selma M. B.; Nascimento, Maria do Desterro Soares Brandão; Orge, Glória; Carvalho, Edgar M.

doi:10.1590/s0074-02762004000500012

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…It is due to the fall of the level of circulating immune complexes after treatment as analysed by antigen specific ELISA (Srivastava 1989). Similarly, the decreases of titer level of the antibodies in cured patient than in the active VL patients have been reported by Kumar et al (2002) and Atta et al (2004), and negativity ranged to 50% and 89% after treatment up to 1 year and 2-8 year respectively according to Hailu (1990). The drug pressure might also have suppressed most of the antibodies against the Leishmanial antigens (Khan et al 1998).…”

Section: Kda Protein Fractionmentioning

confidence: 77%

Identification of An Antigenic and Potential Diagnostic Marker of Leishmania donovani Infection by Immunoblot Assay

Manandhar

2013

Nepal Journal of Science and Technology

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Leishmania donovani parasites from a sodium antimony gluconate (SAG) refractory patients of endemic region were cultured for extraction of crude soluble antigen and performed Western blot with sera from 138 subjects of different groups for diagnosis of Kala azar. The pretreated patients, however, frequently showed 18 bands of different molecular weight, the 85, 74, 63, 31, and 28 kDa were the major proteins against which more than 75% patients developed antibodies. The 74 kDa protein fraction band, which was consistently present in all the pretreated patients(n=35), completely waned out or found in state of virtually absent gloomy band in the six-month follow up patients (n=24). Furthermore, the band was absent in 91% of healthy controls from endemic population(n=33) and was absolutely absent in non-endemic(n=13) and other diseased(n=19) subjects. Although the band was present in 86% of Day 31 subjects(n=14), the average integrated density value (5110±634) of the paired samples was significantly less (p value, <0.0001) than that of Day 0 (11385±1230). The nature of the antigen showed its diagnostic and prognostic importance along with marker for kinetic recovery. Sensitivity and specificity of the protein were absolute in patients and non-endemic population, however, sensitivity was 94% in endemic population. Moreover, it did not show cross-reactivity to different disease groups. Hence, it could be a good diagnostic tool for Kala-azar disease.] Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 63-72 DOI: http://dx.doi.org/10.3126/njst.v13i2.7716

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Section: Kda Protein Fractionmentioning

confidence: 77%

Identification of An Antigenic and Potential Diagnostic Marker of Leishmania donovani Infection by Immunoblot Assay

Manandhar

2013

Nepal Journal of Science and Technology

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“…Silveira et al 27 found this antibody in 64.4% of 955 patients with cutaneous leishmaniasis. Studying patients with visceral leishmaniasis, Atta et al 32 also found anti-Lesihmania antibodies in 100% of the cases; however, only nine patients were included in his study. Pappas et al 33 found positive serology in 98% of 42 patients with visceral leishmaniasis.…”

Section: Discussionmentioning

confidence: 91%

“…In the literature, the predominance of anti-Leishmania antibodies in visceral leishmaniasis can be explained by the evidence of Th2 response in this disease, with polyclonal activation of B cells and production of specific anti-Leishmania and other antibodies with different specificites. 32,34 Among patients with visceral leishmaniasis, the ANA was undetermined in 8.9% of the cases and positive in 4.4% of the cases (titers above 1/160). Other studies comparing the presence of ANA in patients with visceral or cutaneous leishmaniasis were not found in the literature.…”

Section: Discussionmentioning

confidence: 99%

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

Horimoto¹,

Costa²

2009

Rev. Bras. Reumatol.

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Introduction: Leishmaniasis is a chronic infectious disease whose spectrum can vary from isolate cutaneous involvement with oligosymptomatic manifestations to systemic involvement with clinically important manifestations. The development of the infection of each type of leishmaniasis (visceral or cutaneous) depends on a complex and intriguing interaction between virulence factors of the pathogen and the immune response of the host. Analysis of sera of with Leishmania infection demonstrates the presence of autoantibodies against cellular and humoral components, besides circulating immune complexes and anti-IgG antibodies (rheumatoid factor). Patients with visceral leishmaniasis can present symptoms that mimic Systemic Lupus Erythematosus (SLE), hindering early diagnosis and treatment. Objectives: To identify the profile of autoantibodies and complement levels of patients with visceral or cutaneous leishmaniasis and to correlate their clinical presentation to those of patients with SLE. Methods: The presence of autoantibodies and complement levels of 90 patients, 45 with visceral leishmaniasis and 45 with cutaneous leishmaniasis, was determined. Results: The presence of statistically significant autoantibodies in patients with visceral leishmaniasis included: antinuclear antibody (ANA), positive (4.4%) or in low titers (8.9%), and IgG anticardiolipin antibody, positive (17.8%) or undetermined (8.9%). A reduction in C3 levels was also seen in 17.8% of the patients and anti-Leishmania antibodies > 1/80 in all patients with visceral leishmaniasis. Conclusions: Visceral leishmaniasis can have a positive correlation with the presence of autoantibodies, possibly by triggering a predominantly humoral, systemic, type Th2 response, representing an obligatory differential diagnosis with SLE, especially in endemic areas.

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“…Their antigenic reactivity against SNA was mainly represented by weakly stained immunoblots of 14, 29, 60, 77 or 177 kDa. However, these reactions may be caused by crossed immune reactions between IgE antibodies against carbohydrate epitopes and neosporal glycoproteins, as already shown for glycosylated leishmanial antigens (Atta et al., 2004), demonstrating that anti‐neosporal IgE antibodies cannot be used as serum markers of dog N. caninum infection. On the other hand, they showed that neosporal antigens preferentially elicit IgG immune response, in both acute and chronic N. caninum infection.…”

Section: Discussionmentioning

confidence: 96%

Anti‐neosporal IgG and IgE antibodies in Canine Neosporosis

Jesus

Almeida

Atta

2007

Zoonoses and Public Health

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Neospora caninum infection provokes neurological disorders, recurrent abortion and death in dogs and cattle. Dogs are both intermediate and definitive host of N. caninum. Thus, the development of sensitive and specific immunoassays to diagnose canine neosporosis is essential to control this disease. This work investigated serum anti-neosporal IgG and IgE antibodies in 140 dogs represented by 30 healthy animals (group I), 11 dogs showing acute N. caninum infection (group II), 50 urban dogs with serological evidence of canine neosporosis in indirect fluorescent antibody test (IFAT) (group III) and 49 urban dogs without clinical and laboratory evidences of neosporosis (group IV). Enzyme-linked immunosorbent assay (ELISA) and western immunoblotting, both using a soluble N. caninum tachyzoite antigen (SNA), investigated these two isotypes of antibodies, while a Urea-ELISA measured the avidity of the IgG antibodies. Anti-Toxoplasma gondii IgG antibodies were also investigated in the animals. Anti-neosporal IgG was found in all animals from groups II and III, whereas 32.7% (16/49) of dogs from group IV were reactive. IgG antibodies of low avidity were demonstrated in dogs from group II (median 35.3%), while animals from groups III and IV had IgG antibodies of high avidity (medians of 61.5% and 61.7% respectively). IgE antibodies were found in four (13.3%) and five (16.6%) dogs from groups III and IV respectively. Dogs presenting acute infection (group II) or chronic infection (group III) had IgG antibodies to several neosporal antigens, mainly of 29-30 and 35 kDa, while 13 of 16 dogs from group IV recognized antigens from 14 to 170 kDa. Antibodies to T. gondii were detected in 36 of 50 (72%) sera from group III and 25 of 49 (51%) sera from group IV. We concluded that IgG-ELISA and Urea-ELISA with SNA may substitute for IFAT in both laboratory routine and epidemiological studies of canine neosporosis.

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Antileishmanial IgG and IgE antibodies recognize predominantly carbohydrate epitopes of glycosylated antigens in visceral leishmaniasis

Cited by 10 publications

References 22 publications

Identification of An Antigenic and Potential Diagnostic Marker of Leishmania donovani Infection by Immunoblot Assay

Identification of An Antigenic and Potential Diagnostic Marker of Leishmania donovani Infection by Immunoblot Assay

Frequência de autoanticorpos e dosagem de complemento sérico em pacientes com diagnóstico de leishmaniose cutânea ou visceral

Anti‐neosporal IgG and IgE antibodies in Canine Neosporosis

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