The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of Lymnaea columella, L. viatrix, and L. diaphana (Mollusca: Lymnaeidae)

Abstract

The specific identification of Lymnaeid snails is based on a comparison of morphological characters

Lymnaea neotropica and Lymnaea viatrix, potential intermediate hosts for Fascioloides magna

“…Lysis using proteinase K was performed overnight. Once extracted, the internal transcribed spacer (ITS)-1 segment of their nuclear ribosomal DNA was amplified using the following primers: ETTS2 (5 0 -TAAC-AAGGTTTCCGTAGGTGAA-3 0 ) and ITS-1r (5 0 -CGAGC-GAGTGATCCACCGC-3 0 ), for forward and reverse senses, respectively (Carvalho et al, 2004).…”

Anatomical studies of sibling species within neotropical lymnaeids, snail intermediate hosts of fascioliasis

“…ventricosa. More recently, the use of several molecular markers (PCR-RLFP analysis of the ITS1 and ITS2 rDNA and 16S rDNAmt using 12 restriction enzymes) in a study of several populations morphologically identified as L. viatrix from Brazil, Uruguay, and Argentina showed a high level of genetic polymorphism between populations which was interpreted as an intra-specific variation (Carvalho et al 2004).…”

“…ventricosa. More recently, the use of several molecular markers (PCR-RLFP analysis of the ITS1 and ITS2 rDNA and 16S rDNAmt using 12 restriction enzymes) in a study of several populations morphologically identified as L. viatrix from Brazil, Uruguay, and Argentina showed a high level of genetic polymorphism between populations which was interpreted as an intra-specific variation (Carvalho et al 2004).A morphological comparison of several populations of L. cubensis from the Caribbean area and several populations of the introduced L. truncatula from Bolivia was carried out by Samadi et al (2000) using several conchological and anatomical characters of the reproductive system. The conchological study showed the presence of a large amount of variability within and between populations that did not allow distinction between taxa.…”

Synonymy between Lymnaea bogotensis Pilsbry, 1935 and Lymnaea cousini Jousseaume, 1887 (Gastropoda: Lymnaeidae)

“…Paraense (1976aParaense ( , 1982aParaense ( , b, 1983Paraense ( , 1984Paraense ( , 1995Paraense ( , 2004) made the most important taxonomic studies of South American Lymnaea, describing the morphological characters of the reproductive system, kidney, and shell of each species in detail. This methodology was applied by Oviedo et al (1995) and Samadi et al (2000) in their studies on lymneids of the Bolivian plateau, the results of which were confirmed by isoenzyme and molecular techniques (Carvalho et al 2004). Pointier et al (2004) also used this methodology in studies of Venezuelan species.…”