Comparison between splenic and lymph node aspirations as sampling methods for the parasitological detection of Leishmania chagasi infection in dogs

Barrouin‐Melo, Stella Maria; Larangeira, Daniela Farias; Trigo, Joelma; Aguiar, Paulo Henrique Palis; dosSantos, Washington Luis Conrado; Pontes-de-Carvalho, Lain Carlos

doi:10.1590/s0074-02762004000200014

Cited by 46 publications

(35 citation statements)

References 22 publications

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“…000599-08) of FCAV/UNESP, Jaboticabal, state of São Paulo, Brazil. In the present study, the splenic parasite load is according to Melo et al (2004) …”

Section: Ethical Standardsmentioning

confidence: 96%

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Moreira

Franciscato

Rossit

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aim of this study was to evaluate apoptosis and parasite load in the liver and spleen of dogs with visceral leishmaniosis (VL), using immunohistochemistry. Liver and spleen samples from 71 dogs with VL were used. The parasite load in the spleen and liver showed significant difference between organs in infected group (P=0.0219). The density of the parasite load in the spleen (median=2.4) was higher than liver (median=0.8). Immunodetection of apoptotic cells was predominant in lymphocytes and differ between the infected and control group in spleen (P=0.0307) and liver (P=0.0346). There was a significant correlation between apoptosis and parasite load (P = 0.0084; r=0.3104) only in the spleen of the infected group, where it was observed that, when increasing the number of apoptotic cells increases the parasitic load. It was concluded that the liver and spleen of infected dogs presented greater numbers of cells undergoing apoptosis (lymphocytes) than the control group, thus suggesting that this process may be contributing towards the survival of Leishmania in these organs, because lymphocyte in apoptosis did not have the ability to present and recognize the antigen, allowing the survival of the parasite.Keywords: Leishmania infantum, immune escape, apoptosis, dogs. ResumoO objetivo deste estudo foi avaliar a apoptose e a carga parasitária no fígado e baço de cães com leishmaniose visceral (LV), pela técnica de imuno-histoquímica. Foram utilizadas amostras de fígado e baço de 71 cães com LV. A carga parasitária no baço e fígado mostrou diferença significativa entre os órgãos no grupo infectado (P=0,0219). A densidade da carga de parasita no baço (média=2,4) foi maior do que no fígado (média=0,8). A imunodetecção de células em apoptose foi predominante nos linfócitos, com diferenças entre o grupo infectado e controle no baço (P=0,0307) e fígado (P=0,0346). Houve uma correlação positiva fraca entre apoptose e carga parasitária (P=0,0084; r=0,3104) apenas no baço do grupo infectado, onde observou-se que quando aumentava o número de células em apoptose aumentava a carga parasitária. Concluiu-se que o fígado e o baço de cães infectados apresentam um maior número de células que sofrem apoptose (linfócitos) do que o grupo controle, sugerindo que este processo possa contribuir para a sobrevivência de Leishmania nestes órgãos, pois os linfócitos em apoptose não tiveram a capacidade de apresentar e reconhecer o antígeno, permitindo a sobrevivência do parasita.Palavras-chave: Leishmania infantum, evasão da resposta imune, apoptose, cães.

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“…000599-08) of FCAV/UNESP, Jaboticabal, state of São Paulo, Brazil. In the present study, the splenic parasite load is according to Melo et al (2004) …”

Section: Ethical Standardsmentioning

confidence: 96%

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Moreira

Franciscato

Rossit

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…Clinical specimens for the diagnosis of CVL by parasitological examination can be obtained by aspiration of bone marrow, spleen, liver and lymph nodes and, in some cases, by a biopsy of intact skin 15,16 , lesion, or viscera 2,3 .…”

Section: Introductionmentioning

confidence: 99%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

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“…Devido ao intenso parasitismo no baço de animais sintomáticos por LVC, esse órgão foi escolhido por alguns autores (BARROUIN-MELO et al, 2004, 2006 como um excelente local para a obtenção de amostras biológicas e assim, ser explorado para a obtenção de material para o diagnóstico da LVC, por métodos parasitológicos, imunoistoquímicos e outros.…”

Section: Introductionunclassified

Exames parasitológicos, imunoistoquímicos e histopatológicos para detecÇão de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral

Tasca¹,

Buzetti²,

Tenório³

et al. 2009

RBPV

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ResumoO propósito do presente trabalho foi o estudo da Leishmaniose Visceral Canina -LVC por meio de métodos parasitológicos e imunoistoquímicos para a detecção de formas amastigotas de Leishmania (L.) chagasi em baço, além de descrever a histopatologia das lesões esplênicas em 34 cães, com diferentes manifestações clínicas da LVC, eutanasiados pelo Centro de Controle de Zoonoses de Ilha Solteira, SP. Esses animais foram examinados clinicamente antes da eutánásia e de acordo com os sinais clínicos da LVC, foram classificados em três grupos: assintomáticos (8 cães), oligossintomáticos (17 cães) e sintomáticos (9 cães). Após a realização desses exames, dos 34 cães, 22 (64,7%) estavam positivos e 12 (35,3%) negativos. Desses cães positivos, 1/22 (4,5%) era assintomático, 12/22 (54,5%) eram oligossintomáticos e 9/22 (40,1%) sintomáticos. Pela histopatologia, os cães, especialmente os sintomáticos apresentavam o baço com inflamação crônica e espessamento na região capsular e trabecular, além de extensa alteração morfológica na polpa vermelha e branca pela presença de grande quantidade de macrófagos repletos de amastigotas, pela reação granulomatosa inflamatória e pelas áreas hemorrágicas. Os exames histopatológicos e a detecção microscópica direta da L. (L.) chagasi revelaram que o baço é um órgão útil para auxiliar no diagnóstico da LVC. A coloração imunoistoquímica foi a que detectou o maior número de tecidos esplênicos positivos com amastigotas, além de elucidar os casos suspeitos pelos exames parasitológicos, principalmente, nos animais assintomáticos ou oligossintomáticos.Palavras-chave: Leishmania (L.) chagasi, Leishmaniose Visceral, cães, baço, imunoistoquímica. AbstractThe purpose of this work was a Canine Visceral Leishmaniasis -CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest num...

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Comparison between splenic and lymph node aspirations as sampling methods for the parasitological detection of Leishmania chagasi infection in dogs

Cited by 46 publications

References 22 publications

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Exames parasitológicos, imunoistoquímicos e histopatológicos para detecÇão de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral

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