Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae)

Brenière, Simone Frédérique; Taveira, Bruno; Bosseno, Marie-France; Ordoñez, Rosalinda; Lozano-Kasten, Felipe; Magallón-Gastelúm, Ezequiel; Ouaissi, Ali; Ramsey, Janine M.

doi:10.1590/s0074-02762003000800010

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…6,11,14,18 According to the adaptation capacity of Triatomine species to live in human houses, they can be collected inside human dwellings and from peridomestic and sylvatic areas. 9,13 Findings in this study confirmed previous reports that show that T. pallidipennis is an endemic species from the southern region of the State of Mexico.…”

Section: Discussionmentioning

confidence: 99%

“…7 In Mexico, 28 species of hematophagous triatomines have been reported, from which 23 are endemic in some specific regions and the remaining 4 are distributed throughout most of the Chagas-endemic regions of the country. [8][9][10][11] Considering the vectorial transmission capacity and widespread distribution in Mexico, the most important species are Triatoma barberi , Triatoma dimidiata , 12 Triatoma phyllosoma, Triatoma longipennis, Triatoma mazzottii , 12,13 Triatoma pallidipennis, Triatoma picturata , 12,14 Triatoma mexicana, 15 and Triatoma gerstaeckeri . 11 They can be found in altitudes ranging from sea level to 2,500 m in different ecosystems, such as deserts, forests, and mangals.…”

Section: Introductionmentioning

confidence: 99%

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Risk Factors Associated with Triatomines and Its Infection with Trypanosoma cruzi in Rural Communities from the Southern Region of the State of Mexico, Mexico

Medina-Torres¹,

Vázquez-Chagoyán²,

Rodríguez-Vivas

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Abstract. Trypanosoma cruzi prevalence in triatomines and risk factors associated to the presence of the insect were studied in 990 rural houses in the southern region of the State of Mexico, Mexico. In each house, triatomines were collected, and information related to house construction material was obtained. T. cruzi infection was diagnosed in all triatomines. A primary screening was performed using 2 × 2 contingency tables of exposure variables. All variables with P ≤ 0.20 were analyzed by logistic regression. Triatomines ( N = 125) were collected from 822 houses and analyzed for T. cruzi infection. Triatoma pallidipennis (97.4%) and Triatoma dimidiata (2.6%) were identified in 52.1% of the localities and in 6.1% of the houses. Infection was found in 28.0% of triatomines, from which 28.9% were nymphs. Factors associated with triatomine infestation were flooring construction material (dirt floor: odds ratio [OR], 10.05; 95% confidence interval [CI], 5.31-18.04; P = 0.0001), house rooms (at least three rooms: OR, 2.04; 95% CI, 1.07-3.86; P = 0.028), and ceiling construction material (cardboard lamina tile: OR, 6.84; 95% CI, 1.49-31.31; P = 0.013). This study shows T. cruzi circulation in triatomines in the area of study, and because triatomines are adapted for living and reproducing in the domestic environment, there is a potential risk of Chagas disease transmission to humans. Also, we can conclude that the construction materials and house inhabitants are risk factors of triatomines infestation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Risk Factors Associated with Triatomines and Its Infection with Trypanosoma cruzi in Rural Communities from the Southern Region of the State of Mexico, Mexico

Medina-Torres¹,

Vázquez-Chagoyán²,

Rodríguez-Vivas

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…14,15 All of these species are sylvatic, and except for T. dimidiata, they are only endemic to Mexico. 16,17 The most remarkable factor of these investigations with Mexican species is the high rate of infection by T. cruzi , also observed for most of the main South American vectors, such as T. infestans and Triatoma brasiliensis (Neiva, 1911). 11,16 Although several Mexican species have been found in human habitats, it remains difficult to measure the true role of each species in the transmission of disease as there exist few studies on the ecology, geographical distribution, behavior in the human habitat, behavior in the natural medium, and relationship with the prevalence of human infection in corresponding areas.…”

Section: Introductionmentioning

confidence: 97%

“…Furthermore, regarding the Meccus species complex, which includes Meccus longipennis, the species involved in the current study, controversy exists regarding their taxonomic status as species or subspecies because of their close genetic relationships. 17,18 The basic assumption of this work is that a better understanding of Chagas disease in Mexico should consider the zooanthroponotic systems (reservoir-vector-human), which occur in shared spaces. As analyses of entomological features on the community scale are undoubtedly primary to evaluate transmission risk, two rural communities in western Mexico, Los Guerrero (Jalisco) and Cacalután (Nayarit), were studied.…”

Section: Introductionmentioning

confidence: 99%

Community Participation and Domiciliary Occurrence of Infected Meccus longipennis in Two Mexican Villages in Jalisco State

Brenière¹,

Bosseno²,

Gastélum³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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The entomological features of Chagas disease in two western Mexican villages were analyzed through triatomines collection by the inhabitants and active research in the peridomicile. The inhabitant collections have the following comparable characteristics: 1) Meccus longipennis was the dominant species (> 91%), 2) around 43% of the insects were collected indoors, 3) about 70% of triatomines were adults, 4) cumulated rates of infestation of the dwellings reached 40–50%, 5) the triatomine infection rate by Trypanosoma cruzi was > 50%, and 6) the indoor triatomines frequently feed on humans (range 38.5–56.2%). However, the collection was twice as abundant in the first village and the peridomicile infestation, evaluated by the active collection, reached up to 60% and only 4.9% in the other village. Furthermore, females predominated in the first village, whereas males in the other. The current results allow discussing the course of action to prevent Chagas disease in this region.

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“…RAPD requires very small quantities of DNA, and no cloning, sequencing or hybridization are necessary, for this reason, it has a distinct advantage over other molecular techniques generally used for genomic characterization. RAPD technique provided a molecular basis to reassess taxonomic relationships within Triatominae subfamily 5,11 and epidemiologic studies 5 . Furthermore, it has been used in populational genetic characterization of Rhodnius prolixus and Rhodnius columbiensis 12 , Psammolestes tertius 26 , Triatoma dimidiata 7,22 and Triatoma braziliensis 2,3 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae

Fraga

Rodríguez

Fuentes

et al. 2005

Rev. Inst. Med. trop. S. Paulo

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SUMMARYRandom amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of primer, magnesium chloride, template DNA and Taq DNA polymerase with the objective of determining their optimum concentration for the standardization of RAPD technique for genetic studies of Cuban Triatominae. Reproducible amplification patterns were obtained using 5 pmoL of primer, 2.5 mM of MgCl 2 , 25 ng of template DNA and 2 U of Taq DNA polymerase in 25 µL of the reaction. A panel of five random primers was used to evaluate the genetic variability of T. flavida. Three of these (OPA-1, OPA-2 and OPA-4) generated reproducible and distinguishable fingerprinting patterns of Triatominae. Numerical analysis of 52 RAPD amplified bands generated for all five primers was carried out with unweighted pair group method analysis (UPGMA). Jaccard's Similarity Coefficient data were used to construct a dendrogram. Two groups could be distinguished by RAPD data and these groups coincided with geographic origin, i.e. the populations captured in areas from east and west of Guanahacabibes, Pinar del Río. T. flavida present low interpopulation variability that could result in greater susceptibility to pesticides in control programs. The RAPD protocol and the selected primers are useful for molecular characterization of Cuban Triatominae.

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Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae)

Cited by 10 publications

References 11 publications

Risk Factors Associated with Triatomines and Its Infection with Trypanosoma cruzi in Rural Communities from the Southern Region of the State of Mexico, Mexico

Risk Factors Associated with Triatomines and Its Infection with Trypanosoma cruzi in Rural Communities from the Southern Region of the State of Mexico, Mexico

Community Participation and Domiciliary Occurrence of Infected Meccus longipennis in Two Mexican Villages in Jalisco State

Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae

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