Use of molecular methods in identification of Candida Species and evaluation of fluconazole resistance

Isolation and genotyping of vaginal non‐albicans Candida spp. in women from two different ethnic groups in Lanzhou, China

“…Besides identifying fungi species, PCR-RFLP has revealed subtle variations in genotype, and some variations have been associated with strain virulence or susceptibility to antifungal agents [9,10]. Clemons et al [9] found differences in phenotype and virulence between clinically and nonclinically isolated Candida spp.…”

Species-specific detection of Candida tropicalis using evolutionary conserved intein DNA sequences

“…Several molecular methods (restriction fragment length polymorphism (RFLP), arbitrarily primed‐polymerase chain reaction (AP‐PCR), random amplified polymorphic DNA (RAPD), rolling‐circle amplification, real‐time PCR and hybridization probes) have been described for rapid detection of C. tropicalis using the 18s rRNA sequences (Cirak et al . ; Klingspor and Jalal ; Zhou et al . ).…”

Comparison Between Phenotypic Characterization and Semi-Nested PCR for the Identification of Candida Species in Clinical Isolates

“…Among the azoles, fluconazole has revealed tolerance; and antifungal drug resistance is becoming a major problem, especially in immunocompromised patients (15,16). Resistance has been shown to Candida krusei and Candida glabrata (17). Therefore, a rapid and accurate diagnosis of the species may assist in finding an appropriate therapy in the cases of candidiasis and thus reduce the frequency of isolates resistant to antifungal agents such as azoles, which have therapeutic activity against Candida species.…”