Angiostrongylus costaricensis and experimental infection of Sarasinula marginata II: elimination routes

Mendonça, Cristiane L. G. F.; Carvalho, Omar S.; Mota, Eduardo; Pelajo-Machado, Marcelo; Caputo, Luzia Fátima Gonçalves; Lenzi, Henrique Leonel

doi:10.1590/s0074-02762003000700006

Cited by 7 publications

(9 citation statements)

References 9 publications

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“…In S. marginata (Mendonça et al 1999(Mendonça et al , 2003, larvae clearly predominated in fibromuscular tissue, the circulatory system, and kidneys, with occasional invasions of other organs, as was also verified here with Omalonyx sp. Thus, in both species, the migration route is related to the Fig.…”

Section: Discussionsupporting

confidence: 84%

“…The results obtained were compared with those described for S. marginata (Mendonça et al 1999(Mendonça et al , 2003.…”

Section: Histological Study Of Infected Omalonyxmentioning

confidence: 99%

“…Studies on different aspects of the nematode Angiostrongylus costaricensis Morera and Céspedes 1971 infection in mollusk have been carried out by several investigators (Morera and Ash 1971;Conejo and Morera 1988;Thiengo 1996;Rambo et al 1997;Bonetti and Graeff-Teixeira 1998;Mendonça et al 1999Mendonça et al , 2003Banevicius et al 2006). Mollusks from the Veronicellidae family are the main intermediate hosts in endemic areas in Costa Rica (Morera and Ash 1971), Ecuador (Morera et al 1983), Honduras (Kaminsky et al 1987), Nicaragua (Duarte et al 1992), and Brazil (Graeff-Teixeira et al 1989;Rambo et al 1997;Bonetti and Graeff-Teixeira 1998;Laitano et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Montresor

Vidigal

Mendonça³

et al. 2008

Parasitol Res

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Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with stage 1 larvae. Obtained stage 3 larvae were orally inoculated in mice, and after 30 days, adult worms and stage 1 larvae were recovered, demonstrating Omalonyx susceptibility and suitability to infection. To define the parasite migration routes, specimens of Omalonyx with 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 2 days, 5 days, 10 days, 12 days, 15 days, 20 days, 21 days, 25 days, 28 days, and 30 days of infection were fixed and serially sectioned. Histological sections were stained with hematoxylin-eosin. The results were compared to those described in S. marginata. Oral and cutaneous infections were noted. After the penetration, larvae were retained, mainly in the fibromuscular tissue, by hemocytes, or they spread to the whole organism through the circulation, following the anatomical structure of the vasculature. The perilarval hemocyte reaction in Omalonyx was more intense until stage 2 larva instar, decreasing in the presence of stage 3 larvae. Differences in some aspects of hemocyte reaction between S. marginata and Omalonyx exemplify interspecific peculiarities in snail response to the same parasite.

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Section: Discussionsupporting

confidence: 84%

“…The results obtained were compared with those described for S. marginata (Mendonça et al 1999(Mendonça et al , 2003.…”

Section: Histological Study Of Infected Omalonyxmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Montresor

Vidigal

Mendonça³

et al. 2008

Parasitol Res

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“…It has been suggested that muscular contractions in the gastropod headfoot facilitate mechanical disruption of encapsulating granulomas and release of Angiostrongylus costaricensis L3 (9). Emergence may also be enhanced by the proximity of L3 to mucus ducts (32). Variation in the proximity of P. tenuis L3 to mucus ducts may explain the sporadic rather than synchronous nature of larval emergence from snails in our experiments.…”

Section: Discussionmentioning

confidence: 68%

Cathepsin B Homologue at the Interface between a Parasitic Nematode and Its Intermediate Host

et al. 2006

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Parelaphostrongylus tenuis is a parasitic nematode that causes a debilitating neurologic disease in many North American cervids and domestic livestock species. We produced a PCR-based cDNA library from infective larvae (L3) in order to identify molecules that mediate parasitism. A dominant 1,250-bp amplicon encoded a homologue of cathepsin B cysteine proteases. The sequence incorporated a C29G substitution in the putative active site. Antibodies generated against a recombinant form detected the native protein (PtCPR-1) in Western blot assays of L3, but not adult worm, extracts. Immunohistochemical methods revealed that PtCPR-1 synthesis was restricted to larval stages within the snail intermediate host (Triodopsis sp.), beginning as early as 2 days postinfection (dpi) of snails. The protein was present in the intestine and luminal contents and was lost from larvae over time. Concurrent studies showed that larvae induced an immune response in snails beginning at 1 dpi. Layers of hemocytes encapsulated larvae immediately after infection, and granuloma-like structures formed around parasites in chronic infections. Loss of PtCPR-1 from L3 and its accumulation in host tissues coincided with degeneration of granuloma architecture 90 to 105 dpi. Fully developed L3 emerged from the snail at this time. Our data implicate PtCPR-1 in larval development and possibly in the emergence of P. tenuis from the intermediate host. Emerged L3 survived desiccation and cold stress, suggesting that they could remain infectious in the environment. Molecules promoting emergence would facilitate dispersal of L3 and increase the likelihood of transmission to definitive hosts.

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“…Throughout its life cycle, adult worms are located into rodent mesenteric arteries, requiring veronicellidae mollusks as intermediate hosts. The first description on its evolutionary cycle was performed by Morera (1973) and further revisions have added extensive data to the cycle both in vertebrates Lenzi 1995, 2005) and invertebrates (Mendonça et al 1999(Mendonça et al , 2003.…”

Section: Introductionmentioning

confidence: 99%

Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera)

Mendonça¹,

Carvalho²,

Mota

et al. 2008

Parasitol Res

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In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. Some larvae, recovered from sedimentation, were submitted to selective staining after paraffin embedded or inclusion in JB-4 to study inner structures. As control, four slugs were used, two killed at the beginning of infection and the others at the end of the experiment. At day 2 post infection, larvae were motionless and thick, presenting initial retention of granules. At day 4, L2 were detected, persisting until 43 days post infection. Larvae L2 displayed a large amount of granules rich in lipids and carbohydrates through its overall body, with more accumulation at the medial third corresponding to the esophagus-intestine transition site. Lipid granules, the main energetic source, were located at the basal and apical regions of intestinal cells. Both L1 and L3 presented bilateral alae, which is also common in other nematodes. Transition forms between L2 to L3 molts were also observed.

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Angiostrongylus costaricensis and experimental infection of Sarasinula marginata II: elimination routes

Cited by 7 publications

References 9 publications

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

Cathepsin B Homologue at the Interface between a Parasitic Nematode and Its Intermediate Host

Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera)

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