Candida dubliniensis identification in Brazilian yeast stock collection

Abstract

We investigated the presence of Candida dubliniensis among isolates previously identified as Candida albicans and maintained in a yeast stock collection from 1994 to 2000. All isolates were serotyped and further evaluated for antifungal susceptibility profile. After doing a screening test for C. dubliniensis isolates based on the capability of colonies to grow at 42°C, its final identification was obtained by randomly amplified polymorphic DNA (RAPD) analysis using three different primers. A total of 46 out of 548 screened isolates did not exhibit growth at 42°C and were further genotyped by RAPD. Eleven isolates were identified as C. dubliniensis with RAPD analysis. Regarding serotypes, 81.5% of C. albicans and all C. dubliniensis isolates belonged to serotype A. Of note, 9 out of 11 C. dubliniensis isolates were obtained from patients with acquired immunodeficiency syndrome (Aids) and all of them were susceptible to azoles and amphotericin B. We found 17 (3%) C. albicans isolates that were dose-dependent susceptibility or resistant to azoles. In conclusion, we found a low rate of C. dubliniensis isolates among stock cultures of yeasts previously identified as C. albicans. Most of these isolates were recovered from oral samples of Aids patients and exhibited high susceptibility to amphotericin B and azoles. C. albicans serotype A susceptible to all antifungal drugs is the major phenotype found in our stock culture

Oral Candida flora from Brazilian human immunodeficiency virus-infected patients in the highly active antiretroviral therapy era

“…C. albicans serotype-A was the most prevalent phenotype of C. albicans isolates in both groups studied. This result is in accordance with previous reports (Sant 'Ana et al 2002, Mariano et al 2003. Analysis of ubiquinone systems and nucleotide sequences of D1/D2 regions of LSUrDNA -When the authors attempted to identify the yeasts isolated from the oral cavities of HIV-infected patients by using conventional methods, some isolates were difficult to identify.…”

“…Interestingly, no C. dubliniensis isolates were found in our study. C. dubliniensis isolates have been rarely reported in South America, which includes Brazil and Argentina (Rodero et al 1998, Sano et al 2000, Mariano et al 2003) with low incidence of 1.5-2% compared with 14% incidence in worldwide reports (Sullivan et al 1995, Pinjon et al 2003. Rare Candida spp.…”

Candida dubliniensis in a Brazilian family with an HIV 1- infected child: identification, antifungal susceptibility, drug accumulation and sterol composition

“…C. dubliniensis isolates exhibited MIC values of 0.125 µg/mL for voriconazole. C. dubliniensis isolates from Brazil in a previous study (10) reported high susceptibility for azoles and similar results were described in the literature (13,18,19). Nondubliniensis isolates from the other members of the family were susceptible to all drugs tested (data not shown).…”

Asymptomatic oral carriage of Candida species in HIV-infected patients in the highly active antiretroviral therapy era

“…The prevalence of C. dubliniensis isolates in determined populations remains uncommon in HIV-infected patients 19,23,25 . The absence of this species above related, of C. glabrata and only one C. krusei isolate in our group may be explained by the subjects' exposure lack to antifungal agents during the previous three months.…”