Standardization of bovine macrophage monolayers and isolation and culture of trypanosomes

Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves

“…Based on the morphology and cell surface marker expression analyses (19,20), monocyte-derived macrophages in the culture system had an increase in size and granularity and highly expressed CD14 as previous reported (17,(21)(22)(23).…”

Phenotypic, functional, and quantitative characterization of canine peripheral blood monocyte-derived macrophages

“…These results clearly indicate that culture in Teflon flasks resulted in a strong selection for CD14 + cells, which is supposedly achieved by the removal of non-adherent PBMC such as lymphocytes, wich are CD14 -. Saldarriaga et al (2003) found similar results with bovine peripheral blood monocyte-derived macrophages monolayer after 11 days of culture, when 94% of cultured cells were CD14 + . Fig.…”

“…Importantly, mature macrophages from animal tissues are not readily available for culture and functional analysis. Therefore the use of monocytes-derived macrophages for functional studies is a very attractive alternative (Saldarriaga 2003), particularly because it requires a less invasive approach for obtaining the cells when compared to peritoneal or bone marrow-derived macrophages. Such a notion is important considering the animal welfare issues that are currently becoming more significant when designing experiments in which companion animals are studied.…”

“…Unlike monocyte-derived macrophages from other animal species such as porcine, caprine (Wardley et (Olivier et al 2001), and bovine (Saldarriaga et al 2003), canine monocyte-derived macrophages have not been examined in detail in spite of its previous use in research (Kurzman et al 1993, Panaro et al 1998, Sisto et al 2001.…”

Effector Functions of Camelid Heavy-Chain Antibodies in Immunity to West Nile Virus

“…Mononuclear cells were cultured according to the protocol described by Saldarriaga et al (24), with a few modifications. Pelleted cells were resuspended to a final concentration of 2 ϫ 10 6 cells/ml (containing approximately 4 ϫ 10 5 monocytes/ml) in Dulbecco's modified Eagle's medium (Sigma) supplemented with 10% heat-inactivated fetal calf serum (Atlanta Biologicals), 0.5 mg/ml gentamicin sulfate (Cellgro), 0.25 g/ml amphotericin B (Fungizone), 100 U/ml penicillin, 0.1 mg/ml streptomycin, 2 mM L-glutamine, and 50 M ␤-mercaptoethanol (Sigma).…”