A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi

Verbisck, Newton V.; Santos, Marcia Rodrigues dos; Engman, David M.; Chiurillo, Miguel Ángel; Ramı́rez, José Luis; Araya, Jorge E.; Mortara, Renato Arruda; Silveira, José Franco da

doi:10.1590/s0074-02762003000100016

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…In a previous work (Verbisck et al, 2003), we have described a reiterated family of transcribed oligo (A)-terminated interspersed DNA, scattered through the genome of T. cruzi, including the subtelomeric regions. The members of this family (TcSx38, TcSx42, and TcSx12) display the same L1Tc flanking regions contained within rhs genes.…”

Section: T Cruzi Subtelomeres Are a Patchwork Of Blocks Of Rhs And Gmentioning

confidence: 99%

Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres

Dong

Chiurillo

El-Sayed³

et al. 2005

Gene

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Section: T Cruzi Subtelomeres Are a Patchwork Of Blocks Of Rhs And Gmentioning

confidence: 99%

Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres

Dong

Chiurillo

El-Sayed³

et al. 2005

Gene

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“…The possibility that TcML8 could also be a pseudogene cannot be excluded. A family of oligoA-terminated, transcribed DNA elements in the T. cruzi genome has been reported (Verbisck et al 2003). In any case, with a 92% homology, TcML8 is undoubtly related to TcMUC8, so we extended Fig.…”

mentioning

confidence: 97%

A mucin like gene different from the previously reported members of the mucin like gene families is transcribed in Trypanosoma cruzi but not in Trypanosoma rangeli

Abate

Rincón

Díaz-Bello

et al. 2005

Mem. Inst. Oswaldo Cruz

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Trypanosoma cruzi expresses mucin like glycoproteins encoded by a complex multigene family. In this work, we report the transcription in T. cruzi but not in T. rangeli of a mucin type gene automatically annotated by the T. cruzi genome project. The gene showed no nucleotide similarities with the previously reported T. cruzi mucin like genes, although the computational analysis of the deduced protein showed that it has the characteristic features of mucins: a signal peptide sequence, O-glycosylation sites, and glycosylphosphatidylinositol (GPI) anchor sequence. The presence in this gene of N-terminal and C-terminal coding sequences common to other annotated mucin like genes suggests the existence of a new mucin like gene family. Key words: mucin like gene -Trypanosoma cruzi -Trypanosoma rangeliTrypanosoma cruzi, the etiologic agent of Chagas disease, expresses mucin like glycoproteins rich in Thr, Ser, and Pro residues that cover the parasite cell surface (reviewed in Acosta-Serrano et al. 2001). These glycoconjugates are highly O-glycosylated molecules anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) moiety. They represent the major acceptors of sialic acid of the cell-surface trans-sialidase and are involved in parasite infectivity and protection from the immune response of the host. The mucin like genes encode proteins with conserved signal peptides and GPI anchor addition sites, but with highly variable central regions containing the sites for GlcNAc addition. The hundred of genes up to now characterized have been arranged into two separate families on the basis of sequence homology (reviewed in Frasch 2000): the TcMUC family, that contains two subfamilies (TcMUC I and TcMUC II) according to the presence or absence of tandem arrays of a variable number of repeat units, and the TcSMUG family, divided into two groups (Small and Large) according to the size of their encoded mRNAs (Di Noia et al. 2000). It has been recently demonstrated that the TcMUC II group of genes codes for the majority of mucins of the trypomastigote stage and that the C-terminal peptide of TcMUC II mucins elicits strong antibody responses in patients with Chagas disease (Buscaglia et al. 2004).On the other hand, nothing has been reported on mucins in T. rangeli. This is an hemoflagelate species that is considered non-pathogenic for humans but represents a complication for the serological diagnosis of Chagas disease, because the cross reactivity with T. cruzi can produce false positives in the immunological tests used. Serological cross-reactivities can also confuse epidemiological studies of these infections (D'Alessandro & Saravia 1999, Grisard et al. 1999, Guhl & Vallejo 2003. It is still necessary to identify the distinctive characteristics that allow a differential diagnosis and/or epidemiological studies to avoid the unnecessary application of drug treatments that can cause severe side effects in patients with the misdiagnosed disease (Vasquez et al. 1997).As there is evidence for the close evolutive relations...

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A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi

Cited by 2 publications

References 22 publications

Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres

Telomere and subtelomere of Trypanosoma cruzi chromosomes are enriched in (pseudo)genes of retrotransposon hot spot and trans-sialidase-like gene families: the origins of T. cruzi telomeres

A mucin like gene different from the previously reported members of the mucin like gene families is transcribed in Trypanosoma cruzi but not in Trypanosoma rangeli

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