Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata

Caldeira, Roberta Lima; Carvalho, Omar S.; Lage, Regina Coeli Gonçalves; Cardoso, Paula Cristina Marques; Oliveira, Guilherme

doi:10.1590/s0074-02762002000900006

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2007

2022

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Cited by 5 publications

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“…As simple sequence repeats exist in any genome, this method allows fingerprints to be generated for any organism. However, the primers may anneal to sequences other than microsatellites of the desired repeats as in RAPD [ 99 ]. Moreover, the banding patterns can be affected by magnesium ion concentration, thermocycler and annealing temperature in use [ 102 ].…”

Section: Introductionmentioning

confidence: 99%

DNA methods for identification of Chinese medicinal materials

et al. 2007

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As adulterated and substituted Chinese medicinal materials are common in the market, therapeutic effectiveness of such materials cannot be guaranteed. Identification at species-, strain- and locality-levels, therefore, is required for quality assurance/control of Chinese medicine. This review provides an informative introduction to DNA methods for authentication of Chinese medicinal materials. Technical features and examples of the methods based on sequencing, hybridization and polymerase chain reaction (PCR) are described and their suitability for different identification objectives is discussed.

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Section: Introductionmentioning

confidence: 99%