Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test

Matos, Denise C. S.; Marcovistz, Rugimar; Cabello, Pedro Hernán; Georgini, Ricardo Amaral; Sakauchi, Dirce; Silva, Luciana Leite da

doi:10.1590/s0074-02762002000600030

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…The antibody response against Pf MSP-3 recombinant protein and Influenza vaccine were determinate by conventional Enzyme-Linked immunosorbent Assay—ELISA 9 , 10 , and the antibody response against dT vaccine was determined by Toxin Binding Inhibition—ToBI 26 .…”

Section: Methodsmentioning

confidence: 99%

Immune system challenge improves recognition memory and reverses malaria-induced cognitive impairment in mice

Sousa

Ribeiro-Gomes

Almeida

et al. 2021

Sci Rep

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The immune system plays a role in the maintenance of healthy neurocognitive function. Different patterns of immune response triggered by distinct stimuli may affect nervous functions through regulatory or deregulatory signals, depending on the properties of the exogenous immunogens. Here, we investigate the effect of immune stimulation on cognitive-behavioural parameters in healthy mice and its impact on cognitive sequelae resulting from non-severe experimental malaria. We show that immune modulation induced by a specific combination of immune stimuli that induce a type 2 immune response can enhance long-term recognition memory in healthy adult mice subjected to novel object recognition task (NORT) and reverse a lack of recognition ability in NORT and anxiety-like behaviour in a light/dark task that result from a single episode of mild Plasmodium berghei ANKA malaria. Our findings suggest a potential use of immunogens for boosting and recovering recognition memory that may be impaired by chronic and infectious diseases and by the effects of ageing.

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Section: Methodsmentioning

confidence: 99%

Immune system challenge improves recognition memory and reverses malaria-induced cognitive impairment in mice

Sousa

Ribeiro-Gomes

Almeida

et al. 2021

Sci Rep

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“…A new immunoenzymatic test, ViBI, was developed based on our previous work regarding the detection of neutralizing antibodies against tetanus and diphtheria toxins [ 43 ]. Plates were coated with 100 µL of 1 μg/mL 2D12 monoclonal antibody (Bio-Rad, Hercules, CA, USA) in carbonate-bicarbonate buffer pH 9.6 and incubated overnight at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN-γ and CLEC5A

Azamor

Silva

Melgaço

et al. 2021

Viruses

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The yellow fever vaccine (YF17DD) is highly effective with a single injection conferring protection for at least 10 years. The YF17DD induces polyvalent responses, with a TH1/TH2 CD4+ profile, robust T CD8+ responses, and synthesis of interferon-gamma (IFN-γ), culminating in high titers of neutralizing antibodies. Furthermore, C-type lectin domain containing 5A (CLEC5A) has been implicated in innate outcomes in other flaviviral infections. Here, we conducted a follow-up study in volunteers immunized with YF17DD, investigating the humoral response, cellular phenotypes, gene expression, and single nucleotide polymorphisms (SNPs) of IFNG and CLEC5A, to clarify the role of these factors in early response after vaccination. Activation of CLEC5A+ monocytes occurred five days after vaccination (DAV). Following, seven DAV data showed activation of CD4+ and CD8+T cells together with early positive correlations between type II IFN and genes of innate antiviral response (STAT1, STAT2, IRF7, IRF9, OAS1, and RNASEL) as well as antibody levels. Furthermore, individuals with genotypes rs2430561 AT/AA, rs2069718 AG/AA (IFNG), and rs13237944 AC/AA (CLEC5A), exhibited higher expression of IFNG and CLEC5A, respectively. Together, we demonstrated that early IFN-γ and CLEC5A responses, associated with rs2430561, rs2069718, and rs13237944 genotypes, may be key mechanisms in the long-lasting immunity elicited by YF17DD.

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“…In recent years serological models have been developed which can be used as a replacement for lethal challenge procedures in potency testing of Diphtheria and Tetanus Toxoid vaccines (3, 4). Russell and Buch (1959) have introduced the concept of three Rs: replacement, reduction and refinement as a starting-point for ethical behavior concerning experimental animals.…”

mentioning

confidence: 99%

“…Nevertheless, it still remains the gold standard to evaluate the potency of the pertussis component from diphtheria-tetanus toxoid and whole cell pertussis vaccine lots (DTPw). In recent years, serological procedures have been developed to permit the replacement for lethal challenge tests, like the MPT, in the potency control of diphtheria and tetanus components of the DTP vaccine (1, 3, 4). In 1994, van der Ark and collaborators developed an enzyme-linked immunosorbent assay, the Pertussis Serology Potency Test (PSPT), as an unconventional method for pertussis potency control.…”

mentioning

confidence: 99%

Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine

Matos¹,

Marcovistz²,

Silva³

et al. 2012

Braz. J. Microbiol.

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The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production.

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Immunogenicity test of tetanus component in adsorbed vaccines by toxin binding inhibition test

Abstract: Samples from 20 lots of diphtheria-tetanus (adult use dT)

Cited by 10 publications

References 16 publications

Immune system challenge improves recognition memory and reverses malaria-induced cognitive impairment in mice

Immune system challenge improves recognition memory and reverses malaria-induced cognitive impairment in mice

Activation of an Effective Immune Response after Yellow Fever Vaccination Is Associated with the Genetic Background and Early Response of IFN-γ and CLEC5A

Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine

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