Performance of OptiMAL® in the diagnosis of Plasmodium vivax and Plasmodium falciparum infections in a malaria referral center in Colombia

Ferro, Beatriz E.; González, Iveth J.; Carvajal, F; Palma, Gloria; Saravia, Nancy Gore

doi:10.1590/s0074-02762002000500025

Cited by 28 publications

(18 citation statements)

References 12 publications

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“…In addition, the lower sensitivity found for P. falciparum antigens detected by the antibodies used on the OptiMAL strips, in this study, agrees with previous reports (17,18,20,24) with 6-57% differences in sensitivity but with specificities similar to those found in our study. Moody (4), in a large study with 636 patients with malaria symptoms from Sub-Saharan Africa, showed sensitivities for the OptiMAL test of 96, 95.3, 57 and 47% for P. vivax, P. falciparum, P. ovale, and P. malariae, respectively, and specificities of 100% for P. falciparum and 94% for the other species, when compared with the microscopic diagnosis.…”

Section: Discussionsupporting

confidence: 93%

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Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

Rodulfo

Donato

Mora

et al. 2007

Braz J Med Biol Res

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Whole blood samples (N = 295) were obtained from different locations in Amazonas and Sucre States, in Venezuela. Malaria was diagnosed by microscopy, OptiMAL™ and polymerase chain reaction (PCR), with Plasmodium vivax, P. falciparum, and P. malariae being detected when possible. We identified 93 infections, 66 of which were caused by P. vivax, 26 by P. falciparum, and 1 was a mixed infection. No infection caused by P. malariae was detected. The sensitivity and specificity of each diagnostic method were high: 95.7 and 97.9% for microscopy, 87.0 and 97.9% for OptiMAL, and 98.0 and 100% for PCR, respectively. Most samples (72.2%) showed more than 5000 parasites/µL blood. The sensitivity of the diagnosis by microscopy and OptiMAL decreased with lower parasitemia. All samples showing disagreement among the methods were reevaluated, but the first result was used for the calculations. Parasites were detected in the 6 false-negative samples by microscopy after the second examination. The mixed infection was only detected by PCR, while the other methods diagnosed it as P. falciparum (microscopy) or P. vivax (OptiMAL) infection. Most of the false results obtained with the OptiMAL strip were related to the P. falciparum-specific band, including 3 species misdiagnoses, which could be related to the test itself or to genetic variation of the Venezuelan strains. The use of the microscopic method for malaria detection is recommended for its low cost but is very difficult to implement in large scale, population-based studies; thus, we report here more efficient methods suitable for this purpose.

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Section: Discussionsupporting

confidence: 93%

“…Other studies from Thailand, USA, Honduras and Colombia (22)(23)(24)(25) have shown sensitivities and specificities close to 100%, while one study from Canada (26) showed a sensitivity of 29.1 but a specificity of 95.6%.…”

Section: Discussionmentioning

confidence: 96%

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

Rodulfo

Donato

Mora

et al. 2007

Braz J Med Biol Res

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“…To improve the speed and precision of malaria diagnosis in areas where standard laboratory diagnosis is not available, researchers have developed malaria rapid diagnostic tests based on antigen-capture immunochromatographic technologies (12,16). When they are in good condition, some products can achieve sensitivity for the detection of P. falciparum infection similar to that of expert microscopists (3,10). However, the sensitivity can vary between products (14), and a species-specific product is available only for P. falciparum.…”

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confidence: 99%

Detection of Four Plasmodium Species by Genus- and Species-Specific Loop-Mediated Isothermal Amplification for Clinical Diagnosis

Watanabe²,

et al. 2007

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Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria parasites: Plasmodium falciparum, P. vivax, P. malariae, and P. ovale. We evaluated the sensitivity and specificity of LAMP in comparison with the results of microscopic examination and nested PCR. LAMP showed a detection limit (analytical sensitivity) of 10 copies of the target 18S rRNA genes for P. malariae and P. ovale and 100 copies for the genus Plasmodium, P. falciparum, and P. vivax. LAMP detected malaria parasites in 67 of 68 microscopically positive blood samples (sensitivity, 98.5%) and 3 of 53 microscopically negative samples (specificity, 94.3%), in good agreement with the results of nested PCR. The LAMP reactions yielded results within about 26 min, on average, for detection of the genus Plasmodium, 32 min for P. falciparum, 31 min for P. vivax, 35 min for P. malariae, and 36 min for P. ovale. Accordingly, in comparison to the results obtained by microscopy, LAMP had a similar sensitivity and a greater specificity and LAMP yielded results similar to those of nested PCR in a shorter turnaround time. Because it can be performed with a simple technology, i.e., with heat-treated blood as the template, reaction in a water bath, and inspection of the results by the naked eye because of the use of a fluorescent dye, LAMP may provide a simple and reliable test for routine screening for malaria parasites in both clinical laboratories and malaria clinics in areas where malaria is endemic.

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“…Es así que en Colombia se han evaluado en los últimos años pruebas que detectan estos antígenos, como Now ICT Malaria Pf/ Pv ®, Diamed OptiMAL-IT ® Malaria y Paracheck-Pf ®, con el fin de conocer su capacidad diagnóstica, generalmente con buenos resultados (25)(26)(27)(28) Sin embargo, es importante seguir profundizando en la investigación de otros antígenos blanco con el fin de lograr niveles más altos en la sensibilidad y especificidad de las pruebas de diagnóstico rápido, y para evitar diagnósticos con resultados falsos negativos como ocurre en el caso de las cepas de P. falciparum que no expresan la HRP II, por deleción del gen que codifica para este antígeno, situación que se ha presentado principalmente en aislamientos de parásitos de la región amazónica (14,29,30).…”

Section: Discussionunclassified

Evaluación de campo de la precisión diagnóstica de la prueba de diagnóstico rápido SD Bioline Malaria Antigen Pf/Pv® en Colombia

Mendoza¹,

Cucunubá²,

Aponte³

et al. 2013

biomedica

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Performance of OptiMAL® in the diagnosis of Plasmodium vivax and Plasmodium falciparum infections in a malaria referral center in Colombia

Cited by 28 publications

References 12 publications

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

Comparison of the diagnosis of malaria by microscopy, immunochromatography and PCR in endemic areas of Venezuela

Detection of Four Plasmodium Species by Genus- and Species-Specific Loop-Mediated Isothermal Amplification for Clinical Diagnosis

Evaluación de campo de la precisión diagnóstica de la prueba de diagnóstico rápido SD Bioline Malaria Antigen Pf/Pv® en Colombia

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