Experimental Neuroschistosomiasis: Inadequacy of the Murine Model

Silva, Luciana M.; Oliveira, Carla Neves de; Andrade, Zilton A.

doi:10.1590/s0074-02762002000400028

Cited by 12 publications

(7 citation statements)

References 11 publications

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“…Focal egg deposition and granulomatous encephalitis associated with convulsions were seen in an infected cercopithecus monkey (Cheever & Duvall 1974). Recently, Silva et al (2002) have called attention to the inadequacy of the murine model for studies concerning neuroschistosomiasis.…”

Section: Extrahepatic Pathologymentioning

confidence: 99%

Experimental models of Schistosoma mansoni infection

Cheever

Lenzi²,

Lenzi³

et al. 2002

Mem. Inst. Oswaldo Cruz

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121

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Section: Extrahepatic Pathologymentioning

confidence: 99%

Experimental models of Schistosoma mansoni infection

Cheever

Lenzi²,

Lenzi³

et al. 2002

Mem. Inst. Oswaldo Cruz

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121

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“…Besides, this infection alters the level of nerve growth factor (NGF) and causes paw hyperalgesia. However, Silva LM et al [9] have reported different results and revealed the inadequacy of using mouse as an experimental model of NS. In fact, in as early as 1970, Jane JA et al [8] designed their experiments in different conditions, based on the aetiological possibilities of the direct or indirect pathways for NS, but the condition in the experiment was unlikely to occur during the nature course of infection.…”

Section: Discussionmentioning

confidence: 99%

“…Although different from the nature course of infection, this method has several advantages. Firstly, the experimental course is greatly shortened, compared with percutaneous infection course (5-7 weeks) [8][9][10] , since it only takes 1-2 weeks to induce the NS granulomas in the acute stage of infection. Secondly, this method may help exclude many influencing factors.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of a cerebral schistosomiasis experimental model in rabbits

Wang

Chen

et al. 2011

Neurosci. Bull.

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Objective The present study aimed to establish a cerebral schistosomiasis model in rabbits, to provide a valuable tool for morphological analysis, clinical manifestation observation, as well as investigations into immunological reactions and pathogenesis of focal inflammatory reaction in neuroschistosomiasis (NS). Methods Sixty New Zealand rabbits were randomly assigned into operation, sham-operation and normal groups. Rabbits in the operation group received direct injection of dead schistosome eggs into the brain, while their counterparts in the sham-operation group received saline injection. Rabbits in the normal group received no treatment. Base on the clinical manifestations, rabbits were sacrificed on days 3, 5, 7, 10, 20, and 30 post injection, and brain samples were sectioned and stained with hematoxylin-eosin. Sections were observed under the microscope. Results The rabbits in the operation group exhibited various neurological symptoms, including anorexy, partial and general seizures, and paralysis. The morphological analysis showed several schistosome eggs in the nervous tissue on day 3 post operation, with very mild inflammation. On days 7-10 post operation, several schistosome eggs were localized in proximity to red blood cells with many neutrophilic granulocytes and eosinophilic granulocytes around them. The schistosome eggs developed into the productive granuloma stage on days 14-20 post operation. On day 30, the schistosome eggs were found to be in the healing-by-fibrosis stage, and the granuloma area was replaced by fibrillary glia through astrocytosis. The sham-operation group and the normal group showed negative results. Conclusion This method might be used to establish the cerebral schistosomiasis experimental model. Several factors need to be considered in establishing this model, such as the antigenic property of eggs, the time of scarification, and the clinical manifestations.

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“…The ex vivo samples had a catheter placed into the right heart and perfused by a fixative solution of 10% paraformaldehyde (PFA). The worm recovery was carried out as per the technique prescribed by Pellegrino and Siqueira [13][14][15][16][17][18].…”

Section: Mice and Infectionmentioning

confidence: 99%

Murine Model of Neuroschistosomiasis Mansoni: Clinical, Histological and Magnetic Resonance Imaging Studies

Fidelis¹,

Parreiras²,

Moll³

et al. 2018

Adv Tech Biol Med

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The schistosomiasis mansoni infection is responsible for 3.6% of the worldwide estimated causes of death and the central nervous system can be affected. In humans, the eggs of this helminth have been found in the leptomeninges, cerebral cortex, basal ganglia, choroid plexus, cerebellum and spinal cord. Neurological manifestations, histhology and magnetic resonance imaging of neuroschistosomiasis mansoni in humans serve as our chief reference points for the examination of the experimental infections in murine model. In this study, experimental infection of S. mansoni cercariae in mice aims to demonstrate the presence of granulomas in the brain and correlate to the clinical, histologic, and magnetic resonance findings. Twenty five Swiss-webster mice were infected subcutaneously, and followed for 160 days post-infection. Another group of twenty five mice were not infected and kept as controls. Images were obtained in the different planes by magnetic resonance. Histological samples were stained by Hematoxilin and Eosin (HE) to examine S. mansoni eggs, granulomas and inflammatory lesions. The results showed neurological manifestations as head and chest tilt (to the left or right side), hemiparesis, ataxia, body contortion, loss of balance and spinning, induced by granulomas in several regions of the central nervous system, and vascular changes associated with haemorrages. The MRI indicated multiple irregular nodules dispersed associated with oedema. These findings indicate that the murine model subcutaneously infected by S. mansoni cercarie may be used for studying mechanisms leading to human neuroschistosomiasis.

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Experimental Neuroschistosomiasis: Inadequacy of the Murine Model

Abstract: Neuroschistosomiasis is rarely observed in human pathology

Cited by 12 publications

References 11 publications

Experimental models of Schistosoma mansoni infection

Experimental models of Schistosoma mansoni infection

Establishment of a cerebral schistosomiasis experimental model in rabbits

Murine Model of Neuroschistosomiasis Mansoni: Clinical, Histological and Magnetic Resonance Imaging Studies

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