Anopheles albitarsis Embryogenesis: Morphological Identification of Major Events

Monnerat, A.T.; Pelajo-Machado, Marcelo; Vale, Bruno Silva; Soares, Maurílio J.; Lima, José Bento Pereira; Lenzi, Henrique Leonel; Valle, Denise

doi:10.1590/s0074-02762002000400026

Cited by 27 publications

(22 citation statements)

References 21 publications

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“…Additionally, the recent description of An. albitarsis embryogenesis (Monnerat et al 2002) will be taken into account to investigate whether temperature influence is exerted preferentially over any given developmental stage or if, alternatively, embryogenesis is affected as a whole.…”

Section: Discussionmentioning

confidence: 99%

Temperature influence on embryonic development of Anopheles albitarsis and Anopheles aquasalis

Carvalho

Martins

Lima

et al. 2002

Mem. Inst. Oswaldo Cruz

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Section: Discussionmentioning

confidence: 99%

Temperature influence on embryonic development of Anopheles albitarsis and Anopheles aquasalis

Carvalho

Martins

Lima

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…4A); a signal was not found in the full-length eve transcript]. The presence of localisation signals in pair-rule genes of this lower dipteran is interesting, because similar blastoderm types appear to have evolved convergently in the cyclorrhaphan and the culicomorphan branch of Diptera to which Anopheles belongs (Ivanova-Kasas, 1949;Anderson, 1972;Monnerat et al, 2002). Of Empis-eve, Haematopota-h and Haematopota-evetranscripts from species that are more closely related to cyclorrhaphan flies than Anopheles, Clogmia and Coboldiaonly the last localises upon injection into Drosophila.…”

Section: Localisation Signals In Pair-rule Genes Are Evolutionarily Lmentioning

confidence: 99%

Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution

et al. 2004

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Establishment of segmental pattern in the Drosophila syncytial blastoderm embryo depends on pair-rule transcriptional regulators. mRNA transcripts of pair-rule genes localise to the apical cytoplasm of the blastoderm via a selective dynein-based transport system and signals within their 3′-untranslated regions. However, the functional and evolutionary significance of this process remains unknown. We have analysed subcellular localisation of mRNAs from multiple dipteran species both in situ and by injection into Drosophila embryos. We find that although localisation of wingless transcripts is conserved in Diptera, localisation of even-skipped and hairy pair-rule transcripts is evolutionarily labile and correlates with taxon-specific changes in positioning of nuclei. We show in Drosophila that localised pair-rule transcripts target their proteins in close proximity to the nuclei and increase the reliability of the segmentation process by augmenting gene activity. Our data suggest that mRNA localisation signals in pair-rule transcripts affect nuclear protein uptake and thereby adjust gene activity to a variety of dipteran blastoderm cytoarchitectures.

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“…Eggs are fertilized while traversing the genital chamber and begin embryonic development, which normally lasts 2-3 days and is similar to that of Drosophila melanogaster despite notable morphological (1) and molecular (2) differences. Four larval stages (instars) ensue, as compared with three in Drosophila, accompanied by continuous growth.…”

mentioning

confidence: 99%

Life cycle transcriptome of the malaria mosquito Anopheles gambiae and comparison with the fruitfly Drosophila melanogaster

Koutsos

Blass

Meister

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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The African mosquito Anopheles gambiae is the major vector of human malaria. We report a genome-wide survey of mosquito gene expression profiles clustered temporally into developmental programs and spatially into adult tissue-specific patterns. Global expression analysis shows that genes that belong to related functional categories or that encode the same or functionally linked protein domains are associated with characteristic developmental programs or tissue patterns. Comparative analysis of our data together with data published from Drosophila melanogaster reveal an overall strong and positive correlation of developmental expression between orthologous genes. The degree of correlation varies, depending on association of orthologs with certain developmental programs or functional groups. Interestingly, the similarity of gene expression is not correlated with the coding sequence similarity of orthologs, indicating that expression profiles and coding sequences evolve independently. In addition to providing a comprehensive view of temporal and spatial gene expression during the A. gambiae life cycle, this large-scale comparative transcriptomic analysis has detected important evolutionary features of insect transcriptomes.comparative transcriptomics ͉ insect development ͉ insect evolution ͉ microarrays A nopheles gambiae is the major vector of human malaria in subSaharan Africa, a secondary vector of filariasis, and the key vector of O'nyong-nyong viral fever outbreaks. Effective transmission of pathogens results from extreme anthropophilic behavior and repeated bloodfeeding of A. gambiae adult females. Indeed, female mosquitoes are found typically around human habitations and bloodfeed largely on humans rather than animals. Therefore, successful malaria control campaigns to date have coincided largely with local control of Anopheles populations.A substantial bloodmeal is required for A. gambiae egg development, the start of the next life cycle. Eggs are fertilized while traversing the genital chamber and begin embryonic development, which normally lasts 2-3 days and is similar to that of Drosophila melanogaster despite notable morphological (1) and molecular (2) differences. Four larval stages (instars) ensue, as compared with three in Drosophila, accompanied by continuous growth. In that period, larval organs are functional and adult organs incipient or slowly developing. During metamorphosis (from larva to pupa and adult) most larval organs are histolyzed, whereas others persist or grow.To date, molecular and cell biological research on A. gambiae has used D. melanogaster as the model system. These dipterans have diverged from a common ancestor Ϸ250 mya.

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Anopheles albitarsis Embryogenesis: Morphological Identification of Major Events

Abstract: Anopheles albitarsis embryogenesis was analyzed through confocal microscopy of clarified eggs. Using Drosophila melanogaster as reference system, the major morphogenetic events (blastoderm, gastrulation, germ band extension, germ

Cited by 27 publications

References 21 publications

Temperature influence on embryonic development of Anopheles albitarsis and Anopheles aquasalis

Temperature influence on embryonic development of Anopheles albitarsis and Anopheles aquasalis

Differential cytoplasmic mRNA localisation adjusts pair-rule transcription factor activity to cytoarchitecture in dipteran evolution

Life cycle transcriptome of the malaria mosquito Anopheles gambiae and comparison with the fruitfly Drosophila melanogaster

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