Multicenter Brazilian Study of Oral Candida Species Isolated from Aids Patients

Santana, Priscilla de Laet; Milan, Eveline Pı́polo; Martínez, Roberto; Queiroz‐Telles, Flávio; Ferreira, Marcelo Simão; Alcântara, Ana Paula; Carvalho, Miriam T; Colombo, Arnaldo Lopes

doi:10.1590/s0074-02762002000200019

Cited by 45 publications

(44 citation statements)

References 30 publications

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“…With regard to the agents, in 49 (92.45%) of the 53 positive cultures, Candida albicans was identifi ed, and there were two isolates each of C. tropicalis and C. glabrata. C. albicans was the most commonly isolated species, as observed in other studies where the frequency was above 90% (Laet Sant'Ana et al, 2002, QueirozTelles et al, 2002.…”

Section: Resultssupporting

confidence: 66%

Antifungal activity of the extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, against yeasts of the genus Candida isolated from the oral cavity of patients infected with the human immunodeficiency virus

Shinobu-Mesquita¹,

Bertoni²,

Guilhermetti³

et al. 2011

Rev. bras. farmacogn.

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Oropharyngeal candidiasis is the most common fungal infection among patients infected with the human immunodefi ciency virus (HIV), and is treated empirically with topical or systemic antifungals. The objective of the present study was to investigate the possible antifungal action of the hydroalcoholic extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, on yeasts in this population. Samples were collected from HIV-positive patients who attended the Laboratory for Teaching and Research in Clinical Analysis at the Universidade Estadual de Maringá for routine exams. The isolated yeasts were identifi ed at the genus and species levels through classical methodology. Next, tests of microdilution in broth were carried out to determine the profi le of susceptibility of these yeasts towards the hydroalcoholic extract of C. zedoaria, following methodology standardised by the CLSI (2002). A total of 53 yeasts were identifi ed, 49 of them C. albicans, two C. tropicalis and two C. glabrata. These yeasts were inhibited by low concentrations of the extract of C. zedoaria (between 1.95 and 15.63 µg/mL). In addition, 7.82 µg/mL inhibited 90% of the yeasts. Our results indicate a potent antifungal action for C. zedoaria and suggest more detailed studies with a view towards the practical application of this phytomedicine in topical pharmaceutical forms for the treatment of oral candidosis or candidiasis.

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Section: Resultssupporting

confidence: 66%

Antifungal activity of the extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, against yeasts of the genus Candida isolated from the oral cavity of patients infected with the human immunodeficiency virus

Shinobu-Mesquita¹,

Bertoni²,

Guilhermetti³

et al. 2011

Rev. bras. farmacogn.

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“…Although the advent of HAART therapy has produced a decrease in the incidence of opportunistic infections in HIV-positive individuals, 9 in our work we studied oral yeast carriage and found that the patients treated with HAART therapy presented higher frequency of Candida colonisation than the non-treated ones. This could be due to the low number of nontreated patients in comparison with treated ones.…”

Section: Discussionmentioning

confidence: 66%

“…This decrease leads to multiple opportunistic infections such as oral, oesophageal, tracheal and pulmonary candidiasis. 4,9,10 Many of HIV-infected patients have received multiple antifungal treatments for candidiasis or other opportunistic mycoses, and in the last years, cases of oral candidiasis unresponsive to azoles were observed. 11 Development of resistance to fluconazole in immunocompromised patients has become an issue of growing concern and is usually correlated to the degree of immunosuppression and the total dose of drug used in long-term antifungal treatments.…”

Section: Introductionmentioning

confidence: 99%

Oral yeast carriage in HIV‐infected and non‐infected populations in Rosario, Argentina

Luque¹,

Biasoli²,

Tosello³

et al. 2008

Mycoses

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Summary The objectives of the present study were: (i) to assess the frequency of oral colonisation by Candida species in HIV‐positive patients and to compare it with a population of HIV‐negative individuals, (ii) to determine the prevalence of C. dubliniensis in both populations and (iii) to determine the susceptibility of C. dubliniensis and other Candida species isolated from HIV‐positive patients to the most commonly used antifungal agents. Oral samples were obtained from 101 HIV‐positive and 108 HIV‐negative subjects. For yeast identification, we used morphology in cornmeal agar, the API 20C Aux, growth at 45 °C, d‐xylose assimilation, morphology in sunflower seed agar and PCR. The frequency of isolation of Candida in HIV‐positive patients was: C. albicans, 60.7%; C. dubliniensis, 20.2%; C. glabrata, 5.6%; C. krusei, 5.6%; C. tropicalis, 4.5%; others, <5%. The frequency of isolation of Candida in HIV‐negative patients was: C. albicans, 73.9%; C. tropicalis, 15.5%; C. dubliniensis, 2.1%; C. glabrata, 2.1%; C. parapsilosis, 2.1%; others, <5%. The oral colonisation by yeast in the HIV‐positive patients was higher than that in the HIV‐negative subjects. The susceptibilities of 42 Candida isolates to three antifungal agents were determined. All isolates of C. dubliniensis were susceptible to fluconazole, although several individuals had been previously treated with this drug. Out of the 42 Candida isolates, 10 presented resistance to fluconazole and 10 to itraconazole. The presence of Candida species, resistant to commonly used antifungal agents, represents a potential risk in immunocompromised patients.

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“…As espécies de Candida são detectadas em sua maioria na mucosa oral em 40 a 60% da população em geral, principalmente em pacientes infectados pelo HIV. Destas, a C. albicans é a mais comum isolada representando cerca de 80 a 92% dos isolamentos [17][18][19][20][21] . Sant' Ana cols 17 recuperaram de 130 pacientes, 142 isolados de Candida sp da cavidade oral de pacientes com AIDS, sendo que a espécie Candida albicans foi a mais frequentemente isolada (91%).…”

Section: Discussionunclassified

Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso

Favalessa

Martins

Hahn

2010

Rev. Soc. Bras. Med. Trop.

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INTRODUÇÃO: A candidíase é uma das infecções fúngicas mais frequentes entre os pacientes infectados pelo vírus da imunodeficiência humana. O presente estudo objetivou a caracterização das leveduras do gênero Candida de distintas amostras clínicas, provenientes de pacientes HIV - positivos, assim como a determinação do perfil de suscetibilidade in vitro a cinco drogas antifúngicas. MÉTODOS: A caracterização dos isolados de Candida sp foi realizada através da metodologia clássica, testes bioquímicos (zimograma e auxanograma) e morfológicos (prova do tubo germinativo e microcultivo em lâmina). Também, foram realizadas a técnica genotípica (PCR) e identificação pelo método comercial API 20C AUX (BioMeriéux). Para a determinação do perfil de suscetibilidade in vitro, foram utilizadas cinco drogas antifúngicas (cetoconazol, fluconazol, itraconazol, voriconazol e anfotericina B), através do método comercialmente disponível - Etest. RESULTADOS: Foram identificados 105 isolados de leveduras do gênero Candida provenientes de 102 pacientes infectados pelo vírus HIV. Destes, foram caracterizadas 82 (78,1%) Candida albicans, 8 (7,6%) Candida parapsilosis, 8 (7,6%) Candida tropicalis, 4 (3,8%) Candida krusei, 2 (1,9%) Candida glabrata e 1 (1%) Candida guilliermondii. CONCLUSÕES: Considerando o perfil geral de sensibilidade, 60% dos isolados foram suscetíveis a todos os antifúngicos testados, porém as espécies C. tropicalis e C. krusei demonstraram uma tendência a valores mais elevados de CIMs para os azóis do que os encontrados paraC. albicans, sugerindo resistência.

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Multicenter Brazilian Study of Oral Candida Species Isolated from Aids Patients

Abstract: Oropharyngeal candidiasis continues to be considered the most common opportunistic disease in

Cited by 45 publications

References 30 publications

Antifungal activity of the extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, against yeasts of the genus Candida isolated from the oral cavity of patients infected with the human immunodeficiency virus

Antifungal activity of the extract of Curcuma zedoaria (Christm.) Roscoe, Zingiberaceae, against yeasts of the genus Candida isolated from the oral cavity of patients infected with the human immunodeficiency virus

Oral yeast carriage in HIV‐infected and non‐infected populations in Rosario, Argentina

Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso

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