An improved method for concentrating rotavirus from water samples

Kittigul, Leera; Khamoun, Porntip; Sujirarat, Dusit; Utrarachkij, Fuangfa; Chitpirom, Kitja; Chaichantanakit, Nattasai; Vathanophas, Kanda

doi:10.1590/s0074-02762001000600013

Cited by 37 publications

(22 citation statements)

References 19 publications

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“…There are many cases of water-borne epidemics due to rotaviruses (Kittigul et al 2001). These viruses have been isolated in sewage (Dubois et al 1997), river water (Abad et al 1998;Gilgen et al 1997), groundwater (Abbaszadegan et al 1999) and drinking water (Gratacap-Cavallier et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Rotavirus detection in environmental water samples by tangential flow ultrafiltration and RT-nested PCR

Grassi

Bagordo

Idolo

et al. 2009

Environ Monit Assess

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Environmental monitoring was conducted in Otranto (Italy), from January 2006 to April 2007, to monitor the circulation of rotaviruses in various water matrices (raw and treated sewage, surface waters and seawater) and to identify any correlation with the traditional bacteriological indices (faecal coliforms). The viruses were detected using tangential flow ultrafiltration and reverse transcriptase-nested polymerase chain reaction, whilst detection of feaecal coliform was performed according to standard methods. The results showed widespread viral contamination, particularly in spring, of the matrices tested, with the exception of seawater, which at all times tested negative for the presence of rotaviruses. The typing of the rotavirus strains identified the circulation in the studied area of only two genotypes: G1 (22%) and G2 (78%). The bacterial recoveries confirmed the presence of faecal pollution indicators in all examined samples, sometimes with high values. A very weak correlation was found between the presence of faecal coliforms and the circulation of rotaviruses in the environment. The presence of rotaviruses in the environmental water samples may constitute a potential health risk for the local population.

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Section: Introductionmentioning

confidence: 99%

Rotavirus detection in environmental water samples by tangential flow ultrafiltration and RT-nested PCR

Grassi

Bagordo

Idolo

et al. 2009

Environ Monit Assess

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“…All water samples were concentrated using a modified previously described adsorption-elution technique [25]. Briefly, 1 L of water samples were adjusted to pH 3.5 with 1 N HCl, and aluminum chloride was added for a final concentration of 0.0015 N to flocculate the virus.…”

Section: Methodsmentioning

confidence: 99%

Genetic Diversity of Rotavirus Strains Circulating in Environmental Water and Bivalve Shellfish in Thailand

Kittigul

Panjangampatthana

Rupprom

et al. 2014

IJERPH

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Rotavirus is a common cause of acute diarrhea in young children worldwide. This study investigated the prevalence and molecular characterization of rotavirus in environmental water and oyster samples in Thailand. A total of 114 water samples and 110 oyster samples were collected and tested for group A rotavirus using RT-nested PCR. Rotavirus genotype was identified by phylogenetic analysis of the VP7 genetic sequences. Group A rotavirus was detected in 21 water samples (18.4%) and six oyster samples (5.4%). Twenty five rotavirus strains were successfully sequenced and classified into four genotypes; G1, G2, G3, and G9. Rotavirus G1 (three strains), G2 (three strains), and G9 (two strains) demonstrated the genetic sequences similar to human strains (90%–99% nucleotide identity), whereas G3 (17 strains) was closely related to animal strains (84%–98% nucleotide identity). G1 strains belonged to lineages I (sub-lineage c) and II. G2 strains belonged to lineage II. G9 strains belonged to lineages III (sub-lineage b) and IV. G3 strains belonged to lineages I, III (sub-lineage c), and IV with a predominance of lineage I. The present study provides important information on the rotavirus strains circulating in the environment.

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“…Several approaches have been used to increase the concentration of viruses from clinical samples to enhance the sensitivity of detection. [10][11][12] For example, ultracentrifugation and polyethylene glycol (PEG)-mediated precipitation have been used to concentrate a number of different viruses, including adenovirus. Although ultracentrifugation is a well-established procedure for concentrating viruses, it is time consuming and can be incompatible with PCR (ie, increases the number of false-positives).…”

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confidence: 99%

Capturing and concentrating adenovirus using magnetic anionic nanobeads

Sakudo¹,

Baba²,

Ikuta³

2016

IJN

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We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we examined their potential interaction with a nonenveloped virus (adenovirus). The beads were incubated with either adenovirus-infected cell culture medium or nasal aspirates from adenovirus-infected individuals and then separated from the supernatant by applying a magnetic field. After thoroughly washing the beads, adsorption of adenovirus was confirmed by a variety of techniques, including immunochromatography, polymerase chain reaction, Western blotting, and cell culture infection assays. These detection methods positively identified the hexon and penton capsid proteins of adenovirus along with the viral genome on the magnetic beads. Furthermore, various types of adenovirus including Types 5, 6, 11, 19, and 41 were captured using the magnetic bead procedure. Our bead capture method was also found to increase the sensitivity of viral detection. Adenovirus below the detectable limit for immunochromatography was efficiently concentrated using the magnetic bead procedure, allowing the virus to be successfully detected using this methodology. Moreover, these findings clearly demonstrate that a viral envelope is not required for binding to the anionic magnetic beads. Taken together, our results show that this capture procedure increases the sensitivity of detection of adenovirus and would, therefore, be a valuable tool for analyzing both clinical and experimental samples.

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An improved method for concentrating rotavirus from water samples

Cited by 37 publications

References 19 publications

Rotavirus detection in environmental water samples by tangential flow ultrafiltration and RT-nested PCR

Rotavirus detection in environmental water samples by tangential flow ultrafiltration and RT-nested PCR

Genetic Diversity of Rotavirus Strains Circulating in Environmental Water and Bivalve Shellfish in Thailand

Capturing and concentrating adenovirus using magnetic anionic nanobeads

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