Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification

Schistosome/mollusk: genetic compatibility

“…The understanding of the genetic basis for schistosome resistance/or susceptibility in these snail species is especially important, because schistosome eradication programs may benefit from the knowledge of the genetic polymorphism of these snails since association between hosts and parasite tend to generate and maintain genetic polymorphism (May, 1985), as has been predicted in models of schistosome-snail interactions (Morand et al, 1996). Several molecular biology techniques for studies of genetic variability in schistosomiasis vectors have been used Spatz et al, 1999;Knight et al, 1991Knight et al, , 2000Jannotti-Passos and Souza, 2000;Vidigal et al, 2000Vidigal et al, , 2001Caldeira et al, 2000Caldeira et al, , 2001Souza and Passos, 2001;Tuan and Bortolato, 2001). …”

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)

“…Species abbreviations are as defined in Table. DISCUSSION In previous studies, using different molecular techniques, we reported a high degree of intra-specific variability in several species of Biomphalaria from different Brazilian localities, as well as from Uruguay and Argentina (Vidigal e al. 1994, 1996, 1998, Pires et al 1997, Caldeira et al 2001, Campos et al 2002. This variability is also observed when morphological characters such as vaginal pouch, vaginal wrinkling, penis sheath, prepuce, and prostatic diverticula are carefully analyzed (Paraense 1975(Paraense , 1981(Paraense , 1984(Paraense , 1888.…”

Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata

“…Yang et al (2000) used SSR-PCR to differentiate three Metagonimus species parasitizing fish and observed that more polymorphic bands were present when anchored SSR-PCR was used compared to AP-PCR (Yu et al 1997a) and PCR-RFLP (Yu et al 1997b). Caldeira et al (2001), used anchored SSR-PCR to study the genetic variability of the B. straminea complex. The genetic similarity coefficients obtained by these authors were similar to those obtained by Campos et al (2002) for …”

“…The cited investigators named the technique SSR-PCR and obtained successful results with several species of eukaryotes, mainly with the (CA) 8 RY primer, anchored at the 3'end, under high stringency conditions. SSR-PCR has already been applied to studies on intraspecific variability of Trypanosoma cruzi, Leishmania braziliensis and Schistosoma mansoni (Oliveira et al 1997) and to intra-and interspecific studies of snails of the genus Biomphalaria (Caldeira et al 2001, Campos et al 2002. However, a detailed characterization of the amplification targets has never been performed.…”