Detection of the acute phase of abdominal angiostrongyliasis with a parasite-specific IgG enzyme linked immunosorbent assay

Geiger, Stefan Michael; Laitano, Antonio Carlo; Sievers-Tostes, Charlotte; Agostini, Ana Paula; Schulz‐Key, H.; Graeff-Teixeira, Carlos

doi:10.1590/s0074-02762001000400012

Cited by 33 publications

(30 citation statements)

References 9 publications

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“…Compared to a previous report on ELISA using A. costaricensis antigen (Geiger et al 2001), the ELISA using A. cantonensis crude antigen preparation in this study showed increased sensitivity (76% in the previous study vs. 88.4% in this study) and decreased specificity (91.1% in the previous study vs. 78.7% in this study). Purified or cloned antigens have not been established as reliable diagnostic tools.…”

Section: Discussioncontrasting

confidence: 85%

“…costaricensis crude antigens have been used for the immunodiagnosis of angiostrongyliasis since 1997 and later in the diagnosis of acute angiostrongyliasis (Graeff-Teixeira et al 1997, Geiger et al 2001. Previously used antibody-based detection systems included precipitation reactions (Sauerbrey 1977) and latex agglutination using crude antigenic preparations; the latter has been used for many years in Costa Rica (Kramer et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Previously used antibody-based detection systems included precipitation reactions (Sauerbrey 1977) and latex agglutination using crude antigenic preparations; the latter has been used for many years in Costa Rica (Kramer et al 1998). In addition, low molecular weight peptides (Abraham et al 2004), egg antigens (Mesén-Ramírez et al 2008) and crude excretion-secretion antigens, as well as host antibody response at both the class (IgE, IgA) and isotype (IgG1, IgG2, IgG3, IgG4) (Geiger et al 2001) levels, have been studied but not extensively evaluated in clinical or epidemiological settings. Due to insufficient sensitivities, specificities and reproducibilities of these assays in diagnosing A. costaricensis infections and the unavailability of protocols utilizing purified or recombinant antigens for diagnostic purposes, improvements to currently available crude antigen-based ELISA assays are necessary.…”

Section: Discussionmentioning

confidence: 99%

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Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Ben

Rodrigues

Agostini

et al. 2010

Mem. Inst. Oswaldo Cruz

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Angiostrongylus costaricensis has a broad geographic distribution spanning from North to South America and the infections of vertebrates with this nematode can result in abdominal complications. Human infections are diagnosed by histological or serological methods because the isolation of larvae from feces is not feasible, as most parasites become trapped in intestinal tissues due to intense eosinophilic inflammation. Because A. costaricensis is difficult to maintain in the laboratory, an immunodiagnostic IgG enzyme-linked immunosorbent assay (ELISA) using antigens from the congeneric Angiostrongylus cantonensis species was evaluated against a panel of serum samples from patients who were histologically diagnosed with A. costaricensis infections. Sera from uninfected individuals and individuals infected with other parasites were used as controls. The sensitivity and specificity of the assay were estimated at 88.4% and 78.7%, respectively. Because the use of purified or cloned antigens has not been established as a reliable diagnostic tool, the use of heterologous antigens may provide a viable alternative for the development of an ELISA-based immunodetection system for the diagnosis of abdominal angiostrongyliasis

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Section: Discussioncontrasting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Ben

Rodrigues

Agostini

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…The infection cannot be diagnosed in humans using parasitological methods, because man is an abnormal host, and no parasite forms are eliminated in the feces. Attempts at diagnosing the disease by serology have been made with some success 5,7 . DNA probes using PCR-RFLP that differentiate species of the genus Angiostrongylus are been developed 4 , and a PCR in sera for diagnosis has been attempted 22 .…”

Section: Discussionmentioning

confidence: 99%

Human infection by Angiostrongylus costaricensis in Venezuela: first report of a confirmed case

Incani

Caleiras²,

Rodríguez‐Martín³

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYA proven case of human infection caused by Angiostrongylus costaricensis is reported for the first time in Venezuela. The patient was a 57-year-old female surgically operated because of signs of peritonitis with a palpable mass at the lower right quadrant of the abdomen. WBC count reported 16,600 cells/mm 3 , with 46% eosinophils. The tumoral aspect of ileocolic area and peritoneal lymph nodes prompted the resection of a large area of the terminal ileum, cecum, part of the ascending colon and a small part of the jejunum, where a small lesion was found. The pathology showed thickened areas of the intestinal wall with areas of hemorrhage and a perforation of the cecum. Histology showed intense eosinophil infiltration of the whole intestinal wall, granulomas with giant cells and eosinophils. Some of the granuloma surrounded round or oval eggs with content characterized by a large empty area, cells or embryo in the center, and sometimes nematode larvae. A cross section of an adult nematode worm was observed inside a branch of mesenteric artery. The intestinal affected area, the characteristics of the lesions, the presence of eggs in the submucosa with nematode larvae inside, and the observation of a nematode inside a mesenteric artery, makes sufficient criteria for the diagnosis of an infection by Angiostrongylus costaricensis.

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“…ELISA-IgG (76% sensitivity; 91% specificity) was performed as described elsewhere 4 . Briefly, the plates were sensitized with crude antigen of female worms at a concentration of 7µg/ml and serum samples were diluted 1:500 in 0.05% Tween 20, 5% skimmed milk PBS.…”

Section: Methodsmentioning

confidence: 99%

Longitudinal clinical and serological survey of abdominal angiostrongyliasis in Guaporé, southern Brazil, from 1995 to 1999

Graeff-Teixeira

Goulart

Brum

et al. 2005

Rev. Soc. Bras. Med. Trop.

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Abdominal angiostrongyliasis is a zoonotic infection caused by Angiostrongylus costaricensis, a nematode with an intra-vascular location in the mesentery. Our objective was to address several aspects of the natural history of this parasitosis, in a longitudinal clinical and seroepidemiological study. A total of 179 individuals living in a rural area with active transmission in southern Brazil were followed for five years (1995-1999) resulting in yearly prevalence of 28.2%, 4.2%, 10%, 20.2% and 2.8% and incidences of 0%, 5.9%, 8% and 1.5%, respectively. Both men and woman were affected with higher frequencies at age 30-49 years. In 32 individuals serum samples were collected at all time points and IgG antibody reactivity detected by ELISA was variable and usually persisting not longer than one year. Some individual antibody patterns were suggestive of re-infection. There was no association with occurrence of abdominal pain or of other enteroparasites and there was no individual with a confirmed (histopathologic) diagnosis. Mollusks were found with infective third-stage larvae in some houses with an overall prevalence of 16% and a low parasitic burden. In conclusion, abdominal angiostrongyliasis in southern Brazil may be a frequent infection with low morbidity and a gradually decreasing serological reactivity.

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Detection of the acute phase of abdominal angiostrongyliasis with a parasite-specific IgG enzyme linked immunosorbent assay

Cited by 33 publications

References 9 publications

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Use of heterologous antigens for the immunodiagnosis of abdominal angiostrongyliasis by an enzyme-linked immunosorbent assay

Human infection by Angiostrongylus costaricensis in Venezuela: first report of a confirmed case

Longitudinal clinical and serological survey of abdominal angiostrongyliasis in Guaporé, southern Brazil, from 1995 to 1999

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