Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Illnait, Ma.Teresa; Vilaseca, Juan Carlos; Fernández, Carlos M. Fernández; Martínez, García

doi:10.1590/s0074-02762001000200018

Cited by 3 publications

(2 citation statements)

References 21 publications

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“…As a result, the diagnosis of this infection requires more specific and sensitive techniques for faster diagnosis. Serology can provide the fastest diagnosis for this fungi infection [8].…”

Section: Discussionmentioning

confidence: 99%

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Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Safaei

Rezvan

Fateh

et al. 2020

JoMMID

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Introduction: Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone. Methods: The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70 (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA. Results: The antibodies in the rabbit's serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection. Conclusion: The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

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“…As a result, the diagnosis of this infection requires more specific and sensitive techniques for faster diagnosis. Serology can provide the fastest diagnosis for this fungi infection [8].…”

Section: Discussionmentioning

confidence: 99%

“…The prognosis of cryptococcosis depends on early diagnosis, primarily, when the load of the yeast is low. Early diagnosis can assist in the appropriate treatment of the patients and reducing the mortality of the infection [8,9].…”

Section: Introductionmentioning

confidence: 99%

Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Safaei

Rezvan

Fateh

et al. 2020

JoMMID

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2001

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Detection of Cryptococcus by conventional, serological and molecular methods

Saha

Xess

Biswas

et al. 2009

Journal of Medical Microbiology

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The rising incidence of cryptococcosis in India is posing a serious threat. Due to lack of sensitive methods for diagnosis, high morbidity and mortality are associated with the disease. Early diagnosis is essential to prevent serious complications. Therefore, we attempted to find highly sensitive and specific detection methods. A comparative evaluation of the detection of cryptococcosis was done by conventional (direct microscopy and culture) and rapid diagnostic [latex agglutination test (LAT), enzyme immunoassay (EIA) and PCR] methods. The study was done on 359 samples from 52 positive patients and 30 negative controls in an Indian set-up. Evaluation was done for cerebrospinal fluid (CSF), serum and urine separately. The diagnostic value of the tests was assessed in pre-treatment samples, and follow-up tests were also done on samples obtained after initiation of treatment. PCR had the highest sensitivity, followed by EIA and LAT, both before and after treatment. The positive detection by LAT, EIA and PCR was the longest in CSF (.90 days), followed by serum (~65 days) then urine (~45 days) after initiation of treatment. Our results indicated that the sensitivity and specificity of PCR and EIA were comparable in urine, CSF and serum for diagnosis of cryptococcosis.

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Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Cited by 3 publications

References 21 publications

Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Current Awareness

Detection of Cryptococcus by conventional, serological and molecular methods

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