Insect trypanosomatids: the need to know more

Podlipaev, S. A.

doi:10.1590/s0074-02762000000400013

Cited by 52 publications

(47 citation statements)

References 29 publications

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“…A major anticipated caveat on this path is a cultivationimposed selection for faster growing members of mixed infections that occurs in parallel with a counter-selection of more fastidious components (Podlipaev & Frolov, 1987;Podlipaev, 2000Podlipaev, , 2003. Cultures thus obtained would misrepresent original mixed infections.…”

Section: Discussionmentioning

confidence: 99%

“…It was anticipated that an additional problem might be associated with presumed 'mixed' infections (the cases where more than one trypanosomatid species is present simultaneously within the same individual host), especially when the parasites have different cultivation properties (Podlipaev, 2000(Podlipaev, , 2001). An uncultivable or poorly cultivable organism might be predominant in a natural infection, and its morphology would be used to describe a novel species.…”

Section: Introductionmentioning

confidence: 99%

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Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Yurchenko

Lukeš

Jirků

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Mixed trypanosomatid infections (a simultaneous presence of two or more parasites in the same host) have long been suspected to represent an obstacle for recovering cultures that would faithfully represent original species descriptions. However, without the means to directly compare the parasites in the host and in culture, this would remain just a possibility. Here we have used PCR-based genotyping of spliced leader RNA gene repeats to analyse several novel species of insect trypanosomatids isolated from heteropteran hosts and to compare them with the parasites that had been detected in the gut smears of the same hosts. We have found that, whereas the original infections were dominated by some blastocrithidia-like parasites, most of the respective axenic cultures contained novel species of Crithidia and Leptomonas. Therefore, we concluded that, in each case, this replacement was caused by differences in cultivation properties between the original predominant blastocrithidia and the less fastidious parasite that was later recovered in culture. The properties of the new organisms, including their morphology and ultrastructure, as well as their phylogenetic affinities within the family, were investigated and used to describe five novel species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Yurchenko

Lukeš

Jirků

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The similarity between insect and plant flagellates and the trypanosomatids of mammals have excited the interest of biologists for many years (Wallace 1966, McGhee and Cosgrove 1980, Vickerman 1994, Podlipaev 2000. Several reasons emphasizes the importance of the study of lower trypanosomatids such as their possible use as control agents of vectors or pathogens, their use as models in the study of nutrition, biochemistry, ultrastructure, molecular biology, chemotherapeutic agents, symbiosis and bioassays.…”

Section: Lower Trypanosomatidsmentioning

confidence: 99%

The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function

Santos

Branquinha

d’Avila-Levy

2006

An. Acad. Bras. Ciênc.

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Plant and insect trypanosomatids constitute the " lower trypanosomatids", which have been used routinely as laboratory models for biochemical and molecular studies because they are easily cultured under axenic conditions, and they contain homologues of virulence factors from the classic human trypanosomatid pathogens. Among the molecular factors that contribute to Leishmania spp. virulence and pathogenesis, the major surface protease, alternatively called MSP, PSP, leishmanolysin, EC 3.4.24.36 and gp63, is the most abundant surface protein of leishmania promastigotes. A myriad of functions have been described for the gp63 from Leishmania spp. when the metacyclic promastigote is inside the mammalian host. However, less is known about the functions performed by this molecule in the invertebrate vector. Intriguingly, gp63 is predominantly expressed in the insect stage of Leishmania, and in all insect and plant trypanosomatids examined so far. The gp63 homologues found in lower trypanosomatids seem to play essential roles in the nutrition as well as in the interaction with the insect epithelial cells. Since excellent reviews were produced in the last decade regarding the roles played by proteases in the vertebrate hosts, we focused in the recent developments in our understanding of the biochemistry and cell biology of gp63-like proteins in lower trypanosomatids.

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“…Lanes -1: day 7; 2: day 10; 3: day 15; 4: day 21; 5: zone opposite the inoculation day 21; 6: tomato extract control; and 7: SODeTOM; marker proteins of pI, stained with Coomassie Blue. even impossible, because promastigote stages of Leptomonas, Herpetomonas, Crithidia, and Blastocrithidia also occur in Phytomonas vectors (Wallace et al 1992, Camargo & Wallace 1994, Podlipaev 2000, and have sometimes been detected in plant tissues. Thus, it becomes necessary to seek new methods to detect the parasite.…”

Section: Discussionmentioning

confidence: 99%

Identification of excreted iron superoxide dismutase for the diagnosis of Phtytomonas

Marı́n

Rodríguez-González

Sánchez‐Moreno

2006

Mem. Inst. Oswaldo Cruz

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Insect trypanosomatids: the need to know more

Cited by 52 publications

References 29 publications

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function

Identification of excreted iron superoxide dismutase for the diagnosis of Phtytomonas

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