Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility

Bonecini-Almeida, Maria da Glória

doi:10.1590/s0074-02762000000400008

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…The reproducibility is high and it does not require active cell division of mycobacteria. 34,35 The technique, however, needs logistic support from a specialized laboratory that makes it difficult to implement in developing countries. 36 (5) Cultivation detection tests (like MB/Bact, Bactec MGIT 960 systems): in these techniques, biological samples are selectively cultured on solid media to detect and quantify the presence of M. tb.…”

Section: Epidemiologymentioning

confidence: 99%

Biosensor-based detection of tuberculosis

2016

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Section: Epidemiologymentioning

confidence: 99%

Biosensor-based detection of tuberculosis

2016

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“…Listeria monocytogenes and Salmonella typhimurium) to invade the J774A.1 murine macrophage cell line (18). This technique has also been used in studying the biology of M. tuberculosis to identify cell surface antigens (21), M. tuberculosis-secreted proteins, interaction with the immune system (22) and to determine susceptibility to drugs (23,24).…”

Section: Introductionmentioning

confidence: 99%

Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis

Chapeton-Montes

Plaza²,

Barrero³

et al. 2008

Front Biosci

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Current methods for evaluating mycobacterial invasion of target cells pose technical difficulties including a long turn-around time. Thus, new methodologies must be developed that allow rapid and reliable monitoring of host cell invasion. Here, the invasion of A549 cell line by SYBR safe-labeled Mycobacterium tuberculosis (M. tuberculosis) H37Rv was assessed by flow cytometry and was expressed as the percentage of cells infected by M. tuberculosis. Over a third of A549 cells were invaded by M. tuberculosis, two hours after infection. The specificity of the invasion was confirmed in assays using red blood cells as target cells and Escherichia coli as the non-invasive bacterial control. These findings show that invasion of pulmonary epithelial cells by M. tuberculosis can rapidly and quantitatively be assessed with a great sensitivity by flow cytometric detection of SYBR safe-labeled M. tuberculosis.

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Flow Cytometry

Müller¹,

Bley²

2009

Encyclopedia of Industrial Biotechnology

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Microorganisms are widely used in biotechnology and present a wide biodiversity including yeast, algae, protozoa and bacteria. Because of their high abundances, and their large variety of metabolic activities which makes them ubiquitous, microorganisms represent a key component in environmental and biotechnical studies and applications. To enhance our understanding of the heterogeneity and complexity of the microbial consortia, both in term of composition and functioning, their direct analysis must be done on a single cell level and requires a non‐invasive interrogation of the cells. The developments of analytical flow and static cytometry, together with advances in the field of fluorescent probes have enabled the measurement of a wide range of cell characteristics. The study of technical systems where microbial cells interact within an artificially engineered environment (e.g. fermentation, bio‐remediation, bio‐transformation) is important for the biotechnical industry. Accurate measurements relating to cell proliferation, viability and scatter properties of cells are essential if informed decisions about a process are to be made. In this way, products can be harvested at optimal concentrations and inducible systems can be activated at the appropriate time so that high product yields are achieved. In the review are highlighted newly developed techniques and recent advances in this field.

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Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility

Abstract: Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations

Cited by 3 publications

References 34 publications

Biosensor-based detection of tuberculosis

Biosensor-based detection of tuberculosis

Quantitative flow cytometric monitoring of invasion of epithelial cells by Mycobacterium tuberculosis

Flow Cytometry

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