2000
DOI: 10.1590/s0074-02762000000300016
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Immunological evaluation of human immunedeficiency virus infected individuals by flow cytometry

Abstract: Human immunodeficiency virus (HIV) infection heavily compromises the immune system. The decrease of the T cell CD4+ subset along the evolution to acquired immunodeficiency syndrome has been considered as a hallmark of HIV infection. In this paper we review some aspects of the immunopathology of HIV infection and discuss the importance of the flow cytometry for the evaluation of the T lymphocyte subsets in the follow-up of HIV infected children and adults, and for the monitoring of the immune reconstitution upo… Show more

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Cited by 7 publications
(3 citation statements)
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“…Quantification of CD4 + T lymphocytes was performed using Flow Cytometry. 11 Briefly, peripheral blood, collected with anticoagulant, was mixed with red blood cell lysis buffer and incubated with anti- CD4 (BD MultitestTM CD3/CD8/CD45/ CD4 342417). Subsequently, the samples were acquired by the FACSCalibur TM BD cytometer (Beckton, Dickinson and Company, Franklin Lakes, NJ, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Quantification of CD4 + T lymphocytes was performed using Flow Cytometry. 11 Briefly, peripheral blood, collected with anticoagulant, was mixed with red blood cell lysis buffer and incubated with anti- CD4 (BD MultitestTM CD3/CD8/CD45/ CD4 342417). Subsequently, the samples were acquired by the FACSCalibur TM BD cytometer (Beckton, Dickinson and Company, Franklin Lakes, NJ, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Estudos têm demonstrado que a SIDA atinge o sistema imunológico do portador, deixando-o predisposto a infecções oportunistas, neoplasias e problemas neurológicos (COTRAN; KUMAR; ROBBINS, 1996;MORGADO, 2000).…”
Section: Introductionunclassified
“…A presença de pneumonia intersticial linfocítica (LIP) e/ou infecções oportunistas associadas ao HIV no momento do ecocardiograma também foram documentadas.A diagnosticada quando: colesterol total ≥ 170 mg/dL, e/ou colesterol de lipoproteína de baixa densidade ≥ 130 mg/dL, e/ou colesterol de lipoproteína de alta densidade < 45 mg/dL, e/ou triglicerídeos ≥ 130 mg/dL50 .Para a análise quantitativa da carga viral do HIV (cópias/ml), foram utilizadas as seguintes técnicas: Amplificação Sequencial de Ácidos Nucléicos (NASBA)51 , Reação de Polimerização em Cadeia (COBAS AMPLICOR HIV-1 MONITOR versão 1.5 e 2.0 Roche Diagnostics)51 , Técnica de bDNA (branched DNA -SIEMENS)51 e mais recentemente PCR em Tempo Real51 . A contagem de células CD4 foi obtida a partir da citometria de fluxo52 .4.4. Análise EstatísticaPara comparar os grupos 1 e 2, os dados categóricos foram relatados como porcentagens e os dados contínuos, como média (DP) ou mediana (intervalo).…”
unclassified