Immunosuppressive drugs as a tool to explore immunopathology in experimental Chagas disease

Ks, Calabrese

doi:10.1590/s0074-02761999000700046

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…It was reported that CsA exerted its primary influence on CD4 ' T (helper) cells, with indirect effects on other leukocyte populations (Calabrese, 1999;Yang & Guo, 2006). In the present study, chickens treated with CsA had a significantly lower proportion of peripheral blood CD4 ' lymphocyte subsets on day 28 and day 42 and tended to decreased CD8 ' lymphocyte subsets on day 42.…”

Section: Discussionsupporting

confidence: 51%

Conjugated linoleic acids alleviated immunosuppression in broiler chickens exposed to cyclosporin A

Long

Wang

Guo

et al. 2010

Food and Agricultural Immunology

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An experiment with 3)2 factorial arrangement was carried out to determine the effects of dietary conjugated linoleic acids (CLA) on immune function in cyclosporin A (CsA)-immunosuppressed chickens. Two hundred and sixteen one-day-old chickens were randomly allocated into six treatments with CLA (80:20 of c9, t11-CLA:t10, c12-CLA) levels (0, 1.0% and 2.0%) and immunosuppression (treated with CsA or saline with olive oil). CsA treatment significantly (P B0.05) decreased peripheral blood lymphocyte proliferation in response to concanavalin A (Con A) mitogen, CD4 ' lymphocyte subsets and Interleukin-2 (IL-2) production. CLA diets significantly (PB0.05) increased the relative weight of the bursa, lymphocyte proliferation in response to Con A, IL-2 production and CD4 ' lymphocyte subsets. Interaction between CLA and CsA on lymphocyte proliferation in response to Con A and CD4 ' lymphocyte subsets was observed, which indicated that c9, t11-CLA may play a primary role in enhancing immune function under both normal physiological and immunosuppressive conditions.

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Section: Discussionsupporting

confidence: 51%

Conjugated linoleic acids alleviated immunosuppression in broiler chickens exposed to cyclosporin A

Long

Wang

Guo

et al. 2010

Food and Agricultural Immunology

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“…These organs are often the focus of Chagas disease research, since these are the well-characterized disease manifestations. While other studies have identified trypanosomes in liver, spleen and lung tissue (Melo and Brener, 1978) and, more recently, bone and cartilage (Morocoima et al, 2006), these distributions generally follow the use of immunosuppressive therapy (Calabrese et al, 1992;Calabrese, 1999;Taniwaki et al, 2005) to facilitate robust parasite proliferation and expansion. Regardless of the mode of infection or treatment regimen, the sacrifice of animals has typically been required to obtain information on dissemination of parasites and detection of parasites in specific tissues following infection.…”

Section: Introductionmentioning

confidence: 99%

Bioluminescent imaging of Trypanosoma cruzi infection

Hyland

Asfaw

Olson

et al. 2008

International Journal for Parasitology

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Chagas disease, caused by infection with the protozoan parasite Trypanosoma cruzi, is a major public health problem in Central and South America. The pathogenesis of Chagas disease is complex and the natural course of infection is not completely understood. The recent development of bioluminescence imaging technology has facilitated studies of a number of infectious and noninfectious diseases. We developed luminescent T. cruzi to facilitate similar studies of Chagas disease pathogenesis. Luminescent T. cruzi trypomastigotes and amastigotes were imaged in infections of rat myoblast cultures, which demonstrated a clear correlation of photon emission signal strength to the number of parasites used. This was also observed in mice infected with different numbers of luminescent parasites, where a stringent correlation of photon emission to parasite number was observed early at the site of inoculation, followed by dissemination of parasites to different sites over the course of a 25 day infection. Whole animal imaging from ventral, dorsal and lateral perspectives provided clear evidence of parasite dissemination. The tissue distribution of T. cruzi was further determined by imaging heart, spleen, skeletal muscle, lungs, kidneys, liver and intestines ex vivo. These results illustrate the natural dissemination of T. cruzi during infection and unveil a new tool for studying a number of aspects of Chagas disease, including rapid in vitro screening of potential therapeutic agents, roles of parasite and host factors in the outcome of infection, and analysis of differential tissue tropism in various parasite-host strain combinations.

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“…In this way, these cells become more susceptible and die faster. Although no intestinal assessment related to Oxidative Medicine and Cellular Longevity cytokine profile, inflammatory cells, and the presence of the parasite was addressed in the studies included in this work, other studies have analyzed the effect of cyclophosphamide on the heart of animals infected with T. cruzi [43]. In both mice [44][45][46] and dogs [47], the intervention increased the myocarditis process established by the infection.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Treatments for Digestive Forms of Chagas Disease in Experimental Models: A Systematic Review

Neto

Guerra

Rodrigues

et al. 2022

Oxidative Medicine and Cellular Longevity

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Chagas disease is an anthropozoonosis caused by the protozoan Trypanosoma cruzi and is characterized as a neglected disease. It is currently endemic in 21 countries on the Latin American continent, including Bolivia, Argentina, and Paraguay. Unfortunately, there are no optimally effective treatments that can reduce the damage caused in the digestive form of the disease, such as the neuronal destruction of the myenteric plexus of both the esophagus and the colon. Therefore, the objective of this systematic review was to report the possible pharmacological neuroprotective agents that were tested in murine models of the digestive form of Chagas disease. Inclusion criteria are in vivo experimental studies that used different murine models for digestive forms of Chagas disease related to pharmacological interventions with neuroprotective potential, without year and language restriction. On the other hand, the exclusion criteria were studies that did not approach murine models with the digestive form of the disease or did not use neuroprotective treatments, among others. The search in the PubMed, Web of Science, Embase, and LILACS databases was performed on September 4, 2021. In addition, a manual search was performed using the references of the included articles. The risk of bias assessment of the studies was performed based on the SYRCLE tool guidelines, and the data from the selected articles are presented in this review as a narrative description and in tables. Eight articles were included, 4 of which addressed treatment with acetylsalicylic acid, 3 with cyclophosphamide, and 1 with Lycopodium clavatum 13c. In view of the results of the studies, most of them show neuroprotective activity of the treatments, with the potential to reduce the number of damaged neurons, as well as positive changes in the structure of these cells. However, more studies are needed to understand the mechanisms triggered by each drug, as well as their safety and immunogenicity. Systematic review registration is as follows: PROSPERO database (CRD42022289746).

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Immunosuppressive drugs as a tool to explore immunopathology in experimental Chagas disease

Cited by 6 publications

References 21 publications

Conjugated linoleic acids alleviated immunosuppression in broiler chickens exposed to cyclosporin A

Conjugated linoleic acids alleviated immunosuppression in broiler chickens exposed to cyclosporin A

Bioluminescent imaging of Trypanosoma cruzi infection

Neuroprotective Treatments for Digestive Forms of Chagas Disease in Experimental Models: A Systematic Review

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